c-met expression level in primary colon cancer

A predictor of tumor invasion and lymph node metastases

Hiroya Takeuchi, Anton Bilchik, Sukamal Saha, Roderick Turner, David Wiese, Maki Tanaka, Christine Kuo, He Jing Wang, Dave S B Hoon

Research output: Contribution to journalArticle

188 Citations (Scopus)

Abstract

Purpose: Both c-MET and vascular endothelial growth factor (VEGF)-C expression are important factors in primary carcinoma progression. We hypothesized that overexpression of c-MET and/or VEGF-C mRNA in primary colorectal cancer (CRC) can predict tumor invasion and regional metastasis. Experimental Design: The level of c-MET and VEGF-C mRNA expression was assessed using a quantitative RT- RealTime PCR assay on early stage primary CRC tumors (n = 36). Results: The c-MET mRNA copy number ranged from 1.18 × 102 to 1.11 × 106 copies (median 5.17 × 104) per 250 ng of RNA from CRC specimens. c-MET mRNA copies in CRC specimens was significantly higher than that from normal colon mucosal epithelium (P = 0.0001). c-MET mRNA copies significantly correlated with the depth of invasion: T1 versus T2, P = 0.007; T1 versus T3/T4, P = 0.0001; T1 versus T2 versus T3/T4, P = 0.0005; and T1/T2 versus T3/T4, P = 0.011. c-MET copy number in primary CRC of N1/N2 staged patients was significantly higher than No cases (P < 0.03). Expression levels of c-MET mRNA were verified with immunohistochemistry analysis of c-MET protein expression in CRC specimens and normal mucosal epithelium. The VEGF-C mRNA copies of primary CRC assessed ranged from 0 to 1.65 × 105 copies (median 580). AIthough VEGF-C mRNA copies in CRC primary tumors were significantly higher than normal colon mucosal epithelium (P = 0.0008), it did not correlate with any major clinicopathological parameters of CRC. Conclusions: This study indicates c-MET mRNA over-expression in primary CRC may be an important prognostic marker for early stage invasion and regional disease metastasis.

Original languageEnglish
Pages (from-to)1480-1488
Number of pages9
JournalClinical Cancer Research
Volume9
Issue number4
Publication statusPublished - 2003 Apr 1
Externally publishedYes

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Colonic Neoplasms
Colorectal Neoplasms
Lymph Nodes
Neoplasm Metastasis
Vascular Endothelial Growth Factor C
Messenger RNA
Neoplasms
Epithelium
Colon
Research Design
Immunohistochemistry
RNA
Carcinoma
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Takeuchi, H., Bilchik, A., Saha, S., Turner, R., Wiese, D., Tanaka, M., ... Hoon, D. S. B. (2003). c-met expression level in primary colon cancer: A predictor of tumor invasion and lymph node metastases. Clinical Cancer Research, 9(4), 1480-1488.

c-met expression level in primary colon cancer : A predictor of tumor invasion and lymph node metastases. / Takeuchi, Hiroya; Bilchik, Anton; Saha, Sukamal; Turner, Roderick; Wiese, David; Tanaka, Maki; Kuo, Christine; Wang, He Jing; Hoon, Dave S B.

In: Clinical Cancer Research, Vol. 9, No. 4, 01.04.2003, p. 1480-1488.

Research output: Contribution to journalArticle

Takeuchi, H, Bilchik, A, Saha, S, Turner, R, Wiese, D, Tanaka, M, Kuo, C, Wang, HJ & Hoon, DSB 2003, 'c-met expression level in primary colon cancer: A predictor of tumor invasion and lymph node metastases', Clinical Cancer Research, vol. 9, no. 4, pp. 1480-1488.
Takeuchi H, Bilchik A, Saha S, Turner R, Wiese D, Tanaka M et al. c-met expression level in primary colon cancer: A predictor of tumor invasion and lymph node metastases. Clinical Cancer Research. 2003 Apr 1;9(4):1480-1488.
Takeuchi, Hiroya ; Bilchik, Anton ; Saha, Sukamal ; Turner, Roderick ; Wiese, David ; Tanaka, Maki ; Kuo, Christine ; Wang, He Jing ; Hoon, Dave S B. / c-met expression level in primary colon cancer : A predictor of tumor invasion and lymph node metastases. In: Clinical Cancer Research. 2003 ; Vol. 9, No. 4. pp. 1480-1488.
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abstract = "Purpose: Both c-MET and vascular endothelial growth factor (VEGF)-C expression are important factors in primary carcinoma progression. We hypothesized that overexpression of c-MET and/or VEGF-C mRNA in primary colorectal cancer (CRC) can predict tumor invasion and regional metastasis. Experimental Design: The level of c-MET and VEGF-C mRNA expression was assessed using a quantitative RT- RealTime PCR assay on early stage primary CRC tumors (n = 36). Results: The c-MET mRNA copy number ranged from 1.18 × 102 to 1.11 × 106 copies (median 5.17 × 104) per 250 ng of RNA from CRC specimens. c-MET mRNA copies in CRC specimens was significantly higher than that from normal colon mucosal epithelium (P = 0.0001). c-MET mRNA copies significantly correlated with the depth of invasion: T1 versus T2, P = 0.007; T1 versus T3/T4, P = 0.0001; T1 versus T2 versus T3/T4, P = 0.0005; and T1/T2 versus T3/T4, P = 0.011. c-MET copy number in primary CRC of N1/N2 staged patients was significantly higher than No cases (P < 0.03). Expression levels of c-MET mRNA were verified with immunohistochemistry analysis of c-MET protein expression in CRC specimens and normal mucosal epithelium. The VEGF-C mRNA copies of primary CRC assessed ranged from 0 to 1.65 × 105 copies (median 580). AIthough VEGF-C mRNA copies in CRC primary tumors were significantly higher than normal colon mucosal epithelium (P = 0.0008), it did not correlate with any major clinicopathological parameters of CRC. Conclusions: This study indicates c-MET mRNA over-expression in primary CRC may be an important prognostic marker for early stage invasion and regional disease metastasis.",
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AU - Turner, Roderick

AU - Wiese, David

AU - Tanaka, Maki

AU - Kuo, Christine

AU - Wang, He Jing

AU - Hoon, Dave S B

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N2 - Purpose: Both c-MET and vascular endothelial growth factor (VEGF)-C expression are important factors in primary carcinoma progression. We hypothesized that overexpression of c-MET and/or VEGF-C mRNA in primary colorectal cancer (CRC) can predict tumor invasion and regional metastasis. Experimental Design: The level of c-MET and VEGF-C mRNA expression was assessed using a quantitative RT- RealTime PCR assay on early stage primary CRC tumors (n = 36). Results: The c-MET mRNA copy number ranged from 1.18 × 102 to 1.11 × 106 copies (median 5.17 × 104) per 250 ng of RNA from CRC specimens. c-MET mRNA copies in CRC specimens was significantly higher than that from normal colon mucosal epithelium (P = 0.0001). c-MET mRNA copies significantly correlated with the depth of invasion: T1 versus T2, P = 0.007; T1 versus T3/T4, P = 0.0001; T1 versus T2 versus T3/T4, P = 0.0005; and T1/T2 versus T3/T4, P = 0.011. c-MET copy number in primary CRC of N1/N2 staged patients was significantly higher than No cases (P < 0.03). Expression levels of c-MET mRNA were verified with immunohistochemistry analysis of c-MET protein expression in CRC specimens and normal mucosal epithelium. The VEGF-C mRNA copies of primary CRC assessed ranged from 0 to 1.65 × 105 copies (median 580). AIthough VEGF-C mRNA copies in CRC primary tumors were significantly higher than normal colon mucosal epithelium (P = 0.0008), it did not correlate with any major clinicopathological parameters of CRC. Conclusions: This study indicates c-MET mRNA over-expression in primary CRC may be an important prognostic marker for early stage invasion and regional disease metastasis.

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