C-type natriuretic peptide as a new regulator of food intake and energy expenditure

Megumi Inuzuka, Naohisa Tamura, Nobuko Yamada, Goro Katsuura, Naofumi Oyamada, Daisuke Taura, Takuhiro Sonoyama, Yasutomo Fukunaga, Kousaku Ohinata, Masakatsu Sone, Kazuwa Nakao

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The physiological implication of C-type natriuretic peptide (CNP) including energy metabolism has not been elucidated, because of markedly short stature in CNP-null mice. In the present study we analyzed food intake and energy expenditure of CNP-null mice with chondrocyte-targeted CNP expression (CNP-Tg/Nppc-/- mice), in which marked skeletal dysplasia was rescued, to investigate the significance of CNP under minimal influences of skeletal phenotypes. In CNP-Tg/Nppc-/- mice, body weight and body fat ratio were reduced by 24% and 32%, respectively, at 20 wk of age, and decreases of blood glucose levels during insulin tolerance tests were 2-fold exaggerated at 17 wk of age, as compared with CNP-Tg/Nppc+/+ mice. Urinary noradrenalin excretion of CNP-Tg/Nppc-/- mice was greater than that of CNP-Tg/Nppc+/+ mice by 28%. In CNP-Tg/Nppc-/- mice, rectal temperature at 1600 h was higher by 1.1 C, and uncoupling protein-1 mRNA expression in the brown adipose tissue was 2-fold increased, which was canceled by propranolol administration, as compared with CNP-Tg/Nppc+/+ mice. Oxygen consumption was significantly increased in CNP-Tg/Nppc-/- mice compared with that in CNP-Tg/Nppc+/+ mice. Food intake of CNP-Tg/Nppc-/- mice upon ad libitum feeding and refeeding after 48 h starvation were reduced by 21% and 61%, respectively, as compared with CNP-Tg/Nppc+/+ mice. This study unveiled a new aspect of CNP as a molecule regulating food intake and energy expenditure. Further analyses on precise mechanisms of CNP actions would lead to the better understanding of the significance of the CNP/guanylyl cyclase-B system in food intake and energy expenditure.

Original languageEnglish
Pages (from-to)3633-3642
Number of pages10
JournalEndocrinology
Volume151
Issue number8
DOIs
Publication statusPublished - 2010 Aug
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology

Fingerprint Dive into the research topics of 'C-type natriuretic peptide as a new regulator of food intake and energy expenditure'. Together they form a unique fingerprint.

  • Cite this

    Inuzuka, M., Tamura, N., Yamada, N., Katsuura, G., Oyamada, N., Taura, D., Sonoyama, T., Fukunaga, Y., Ohinata, K., Sone, M., & Nakao, K. (2010). C-type natriuretic peptide as a new regulator of food intake and energy expenditure. Endocrinology, 151(8), 3633-3642. https://doi.org/10.1210/en.2010-0141