C-type natriuretic peptide as a new regulator of food intake and energy expenditure

Megumi Inuzuka, Naohisa Tamura, Nobuko Goto, Goro Katsuura, Naofumi Oyamada, Daisuke Taura, Takuhiro Sonoyama, Yasutomo Fukunaga, Kousaku Ohinata, Masakatsu Sone, Kazuwa Nakao

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Abstract

The physiological implication of C-type natriuretic peptide (CNP) including energy metabolism has not been elucidated, because of markedly short stature in CNP-null mice. In the present study we analyzed food intake and energy expenditure of CNP-null mice with chondrocyte-targeted CNP expression (CNP-Tg/Nppc-/- mice), in which marked skeletal dysplasia was rescued, to investigate the significance of CNP under minimal influences of skeletal phenotypes. In CNP-Tg/Nppc-/- mice, body weight and body fat ratio were reduced by 24% and 32%, respectively, at 20 wk of age, and decreases of blood glucose levels during insulin tolerance tests were 2-fold exaggerated at 17 wk of age, as compared with CNP-Tg/Nppc+/+ mice. Urinary noradrenalin excretion of CNP-Tg/Nppc-/- mice was greater than that of CNP-Tg/Nppc+/+ mice by 28%. In CNP-Tg/Nppc-/- mice, rectal temperature at 1600 h was higher by 1.1 C, and uncoupling protein-1 mRNA expression in the brown adipose tissue was 2-fold increased, which was canceled by propranolol administration, as compared with CNP-Tg/Nppc+/+ mice. Oxygen consumption was significantly increased in CNP-Tg/Nppc-/- mice compared with that in CNP-Tg/Nppc+/+ mice. Food intake of CNP-Tg/Nppc-/- mice upon ad libitum feeding and refeeding after 48 h starvation were reduced by 21% and 61%, respectively, as compared with CNP-Tg/Nppc+/+ mice. This study unveiled a new aspect of CNP as a molecule regulating food intake and energy expenditure. Further analyses on precise mechanisms of CNP actions would lead to the better understanding of the significance of the CNP/guanylyl cyclase-B system in food intake and energy expenditure.

Original languageEnglish
Pages (from-to)3633-3642
Number of pages10
JournalEndocrinology
Volume151
Issue number8
DOIs
Publication statusPublished - 2010 Aug 1
Externally publishedYes

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C-Type Natriuretic Peptide
Energy Metabolism
Eating

ASJC Scopus subject areas

  • Endocrinology

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Inuzuka, M., Tamura, N., Goto, N., Katsuura, G., Oyamada, N., Taura, D., ... Nakao, K. (2010). C-type natriuretic peptide as a new regulator of food intake and energy expenditure. Endocrinology, 151(8), 3633-3642. https://doi.org/10.1210/en.2010-0141

C-type natriuretic peptide as a new regulator of food intake and energy expenditure. / Inuzuka, Megumi; Tamura, Naohisa; Goto, Nobuko; Katsuura, Goro; Oyamada, Naofumi; Taura, Daisuke; Sonoyama, Takuhiro; Fukunaga, Yasutomo; Ohinata, Kousaku; Sone, Masakatsu; Nakao, Kazuwa.

In: Endocrinology, Vol. 151, No. 8, 01.08.2010, p. 3633-3642.

Research output: Contribution to journalArticle

Inuzuka, M, Tamura, N, Goto, N, Katsuura, G, Oyamada, N, Taura, D, Sonoyama, T, Fukunaga, Y, Ohinata, K, Sone, M & Nakao, K 2010, 'C-type natriuretic peptide as a new regulator of food intake and energy expenditure', Endocrinology, vol. 151, no. 8, pp. 3633-3642. https://doi.org/10.1210/en.2010-0141
Inuzuka, Megumi ; Tamura, Naohisa ; Goto, Nobuko ; Katsuura, Goro ; Oyamada, Naofumi ; Taura, Daisuke ; Sonoyama, Takuhiro ; Fukunaga, Yasutomo ; Ohinata, Kousaku ; Sone, Masakatsu ; Nakao, Kazuwa. / C-type natriuretic peptide as a new regulator of food intake and energy expenditure. In: Endocrinology. 2010 ; Vol. 151, No. 8. pp. 3633-3642.
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