C-type natriuretic peptide as a possible local modulator of aldosterone secretion in bovine adrenal zona glomerulosa

Mayumi Kawai, Mitsuhide Naruse, Takanobu Yoshimoto, Kiyoko Naruse, Kiseko Shionoya, Masami Tanaka, Yoshikazu Morishita, Yuzuru Matsuda, Reiko Demura, Hiroshi Demura

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Although atrial and brain natriuretic peptides are well known to be involved in the regulation of cardiovascular and endocrine functions as circulating hormones, the roles of the C-type natriuretic peptide (CNP) remain unknown. We examined the effects of CNP on the secretion of aldosterone and cyclic nucleotides from bovine adrenal zona glomerulosa cells in culture. CNP produced a dose-dependent increase in the basal secretion of cGMP, with an EC50 of 3.8 x 10-10 M. CNP significantly inhibited the ACTH-induced increase in aldosterone and cAMP in a dose-related manner, with an IC50 of 3.6 x 10-10 M. Although ACTH itself did not increase cGMP secretion, the addition of CNP elicited a significant increase in cGMP secretion. The effects of CNP on the basal secretion of cGMP and the ACTH- induced secretion of aldosterone were significantly reversed by a nonpeptide natriuretic peptide receptor antagonist, HS-142-1. CNP immunoreactivity was localized in the zona glomerulosa by immunohistochemical staining. In addition, expression of CNP messenger RNA and natriuretic peptide B receptor messenger RNA was demonstrated by RT-PCR in the zona glomerulosa tissue and cells in culture. These findings suggest that CNP is a local factor regulating ACTH-induced aldosterone secretion through a guanylyl cyclase- cGMP pathway.

Original languageEnglish
Pages (from-to)42-46
Number of pages5
JournalEndocrinology
Volume137
Issue number1
DOIs
Publication statusPublished - 1996 Jan 1
Externally publishedYes

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C-Type Natriuretic Peptide
Zona Glomerulosa
Aldosterone
Adrenocorticotropic Hormone
Cell Culture Techniques
Messenger RNA
Natriuretic Peptides
Peptide Receptors
Guanylate Cyclase
Brain Natriuretic Peptide
Cyclic Nucleotides
Atrial Natriuretic Factor
Inhibitory Concentration 50
Hormones
Staining and Labeling

ASJC Scopus subject areas

  • Endocrinology

Cite this

C-type natriuretic peptide as a possible local modulator of aldosterone secretion in bovine adrenal zona glomerulosa. / Kawai, Mayumi; Naruse, Mitsuhide; Yoshimoto, Takanobu; Naruse, Kiyoko; Shionoya, Kiseko; Tanaka, Masami; Morishita, Yoshikazu; Matsuda, Yuzuru; Demura, Reiko; Demura, Hiroshi.

In: Endocrinology, Vol. 137, No. 1, 01.01.1996, p. 42-46.

Research output: Contribution to journalArticle

Kawai, M, Naruse, M, Yoshimoto, T, Naruse, K, Shionoya, K, Tanaka, M, Morishita, Y, Matsuda, Y, Demura, R & Demura, H 1996, 'C-type natriuretic peptide as a possible local modulator of aldosterone secretion in bovine adrenal zona glomerulosa', Endocrinology, vol. 137, no. 1, pp. 42-46. https://doi.org/10.1210/endo.137.1.8536640
Kawai, Mayumi ; Naruse, Mitsuhide ; Yoshimoto, Takanobu ; Naruse, Kiyoko ; Shionoya, Kiseko ; Tanaka, Masami ; Morishita, Yoshikazu ; Matsuda, Yuzuru ; Demura, Reiko ; Demura, Hiroshi. / C-type natriuretic peptide as a possible local modulator of aldosterone secretion in bovine adrenal zona glomerulosa. In: Endocrinology. 1996 ; Vol. 137, No. 1. pp. 42-46.
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