Abstract
Background: C-type natriuretic peptide (CNP) belongs to the natriuretic peptide family and is considered to have regulatory effects on vascular tone and smooth muscle growth. Since these features play a role in atherogenesis, the presence of CNP at such sites was studied. Methods and Results: Thirty- three coronary artery segments were harvested at autopsy: 10 normal, with diffuse intimal thickening, and 23 atherosclerotic lesions. Samples were snap-frozen and processed for immunohistochemical staining. For the identification of CNP, a mouse monoclonal antibody (KY-CNP-1) was used. 1A4, EBM-11 (CD68), and von Willebrand factor antibodies were used to stain smooth muscle cells, macrophages, and endothelial cells, respectively. CNP is present in several cell types. Normal arterial segments show CNP-positive endothelial cells. Hypercellular atherosclerotic lesions show distinct CNP positivity of smooth muscle cells and macrophages but a decrease in positivity of endothelial cells. Advanced atherosclerotic lesions contain CNP-positive macrophages, but the smooth muscle cells within the fibrous cap and the surface endothelial cells are almost all CNP negative. Conclusions: These observations suggest that CNP has functional significance in atherogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 3103-3108 |
| Number of pages | 6 |
| Journal | Circulation |
| Volume | 94 |
| Issue number | 12 |
| Publication status | Published - 1996 |
| Externally published | Yes |
Fingerprint
Keywords
- atherosclerosis
- muscle, smooth
- natriuretic peptides
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine
Cite this
C-type natriuretic peptide in human coronary atherosclerotic lesions. / Naruko, Takahiko; Ueda, Makiko; Van der Wal, Allard C.; Van der Loos, Chris M.; Itoh, Hiroshi; Nakao, Kazuwa; Becker, Anton E.
In: Circulation, Vol. 94, No. 12, 1996, p. 3103-3108.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - C-type natriuretic peptide in human coronary atherosclerotic lesions
AU - Naruko, Takahiko
AU - Ueda, Makiko
AU - Van der Wal, Allard C.
AU - Van der Loos, Chris M.
AU - Itoh, Hiroshi
AU - Nakao, Kazuwa
AU - Becker, Anton E.
PY - 1996
Y1 - 1996
N2 - Background: C-type natriuretic peptide (CNP) belongs to the natriuretic peptide family and is considered to have regulatory effects on vascular tone and smooth muscle growth. Since these features play a role in atherogenesis, the presence of CNP at such sites was studied. Methods and Results: Thirty- three coronary artery segments were harvested at autopsy: 10 normal, with diffuse intimal thickening, and 23 atherosclerotic lesions. Samples were snap-frozen and processed for immunohistochemical staining. For the identification of CNP, a mouse monoclonal antibody (KY-CNP-1) was used. 1A4, EBM-11 (CD68), and von Willebrand factor antibodies were used to stain smooth muscle cells, macrophages, and endothelial cells, respectively. CNP is present in several cell types. Normal arterial segments show CNP-positive endothelial cells. Hypercellular atherosclerotic lesions show distinct CNP positivity of smooth muscle cells and macrophages but a decrease in positivity of endothelial cells. Advanced atherosclerotic lesions contain CNP-positive macrophages, but the smooth muscle cells within the fibrous cap and the surface endothelial cells are almost all CNP negative. Conclusions: These observations suggest that CNP has functional significance in atherogenesis.
AB - Background: C-type natriuretic peptide (CNP) belongs to the natriuretic peptide family and is considered to have regulatory effects on vascular tone and smooth muscle growth. Since these features play a role in atherogenesis, the presence of CNP at such sites was studied. Methods and Results: Thirty- three coronary artery segments were harvested at autopsy: 10 normal, with diffuse intimal thickening, and 23 atherosclerotic lesions. Samples were snap-frozen and processed for immunohistochemical staining. For the identification of CNP, a mouse monoclonal antibody (KY-CNP-1) was used. 1A4, EBM-11 (CD68), and von Willebrand factor antibodies were used to stain smooth muscle cells, macrophages, and endothelial cells, respectively. CNP is present in several cell types. Normal arterial segments show CNP-positive endothelial cells. Hypercellular atherosclerotic lesions show distinct CNP positivity of smooth muscle cells and macrophages but a decrease in positivity of endothelial cells. Advanced atherosclerotic lesions contain CNP-positive macrophages, but the smooth muscle cells within the fibrous cap and the surface endothelial cells are almost all CNP negative. Conclusions: These observations suggest that CNP has functional significance in atherogenesis.
KW - atherosclerosis
KW - muscle, smooth
KW - natriuretic peptides
UR - http://www.scopus.com/inward/record.url?scp=0030452795&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030452795&partnerID=8YFLogxK
M3 - Article
C2 - 8989116
AN - SCOPUS:0030452795
VL - 94
SP - 3103
EP - 3108
JO - Circulation
JF - Circulation
SN - 0009-7322
IS - 12
ER -