C-type natriuretic peptide in human coronary atherosclerotic lesions

Takahiko Naruko, Makiko Ueda, Allard C. Van der Wal, Chris M. Van der Loos, Hiroshi Itoh, Kazuwa Nakao, Anton E. Becker

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Background: C-type natriuretic peptide (CNP) belongs to the natriuretic peptide family and is considered to have regulatory effects on vascular tone and smooth muscle growth. Since these features play a role in atherogenesis, the presence of CNP at such sites was studied. Methods and Results: Thirty- three coronary artery segments were harvested at autopsy: 10 normal, with diffuse intimal thickening, and 23 atherosclerotic lesions. Samples were snap-frozen and processed for immunohistochemical staining. For the identification of CNP, a mouse monoclonal antibody (KY-CNP-1) was used. 1A4, EBM-11 (CD68), and von Willebrand factor antibodies were used to stain smooth muscle cells, macrophages, and endothelial cells, respectively. CNP is present in several cell types. Normal arterial segments show CNP-positive endothelial cells. Hypercellular atherosclerotic lesions show distinct CNP positivity of smooth muscle cells and macrophages but a decrease in positivity of endothelial cells. Advanced atherosclerotic lesions contain CNP-positive macrophages, but the smooth muscle cells within the fibrous cap and the surface endothelial cells are almost all CNP negative. Conclusions: These observations suggest that CNP has functional significance in atherogenesis.

Original languageEnglish
Pages (from-to)3103-3108
Number of pages6
JournalCirculation
Volume94
Issue number12
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

C-Type Natriuretic Peptide
Endothelial Cells
Smooth Muscle Myocytes
Macrophages
Atherosclerosis
Tunica Intima
Natriuretic Peptides
von Willebrand Factor
Vascular Smooth Muscle
Autopsy
Coronary Vessels
Coloring Agents
Monoclonal Antibodies

Keywords

  • atherosclerosis
  • muscle, smooth
  • natriuretic peptides

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Naruko, T., Ueda, M., Van der Wal, A. C., Van der Loos, C. M., Itoh, H., Nakao, K., & Becker, A. E. (1996). C-type natriuretic peptide in human coronary atherosclerotic lesions. Circulation, 94(12), 3103-3108.

C-type natriuretic peptide in human coronary atherosclerotic lesions. / Naruko, Takahiko; Ueda, Makiko; Van der Wal, Allard C.; Van der Loos, Chris M.; Itoh, Hiroshi; Nakao, Kazuwa; Becker, Anton E.

In: Circulation, Vol. 94, No. 12, 1996, p. 3103-3108.

Research output: Contribution to journalArticle

Naruko, T, Ueda, M, Van der Wal, AC, Van der Loos, CM, Itoh, H, Nakao, K & Becker, AE 1996, 'C-type natriuretic peptide in human coronary atherosclerotic lesions', Circulation, vol. 94, no. 12, pp. 3103-3108.
Naruko T, Ueda M, Van der Wal AC, Van der Loos CM, Itoh H, Nakao K et al. C-type natriuretic peptide in human coronary atherosclerotic lesions. Circulation. 1996;94(12):3103-3108.
Naruko, Takahiko ; Ueda, Makiko ; Van der Wal, Allard C. ; Van der Loos, Chris M. ; Itoh, Hiroshi ; Nakao, Kazuwa ; Becker, Anton E. / C-type natriuretic peptide in human coronary atherosclerotic lesions. In: Circulation. 1996 ; Vol. 94, No. 12. pp. 3103-3108.
@article{89e9aa22a63c43a5a5f7c266ceb3f3e5,
title = "C-type natriuretic peptide in human coronary atherosclerotic lesions",
abstract = "Background: C-type natriuretic peptide (CNP) belongs to the natriuretic peptide family and is considered to have regulatory effects on vascular tone and smooth muscle growth. Since these features play a role in atherogenesis, the presence of CNP at such sites was studied. Methods and Results: Thirty- three coronary artery segments were harvested at autopsy: 10 normal, with diffuse intimal thickening, and 23 atherosclerotic lesions. Samples were snap-frozen and processed for immunohistochemical staining. For the identification of CNP, a mouse monoclonal antibody (KY-CNP-1) was used. 1A4, EBM-11 (CD68), and von Willebrand factor antibodies were used to stain smooth muscle cells, macrophages, and endothelial cells, respectively. CNP is present in several cell types. Normal arterial segments show CNP-positive endothelial cells. Hypercellular atherosclerotic lesions show distinct CNP positivity of smooth muscle cells and macrophages but a decrease in positivity of endothelial cells. Advanced atherosclerotic lesions contain CNP-positive macrophages, but the smooth muscle cells within the fibrous cap and the surface endothelial cells are almost all CNP negative. Conclusions: These observations suggest that CNP has functional significance in atherogenesis.",
keywords = "atherosclerosis, muscle, smooth, natriuretic peptides",
author = "Takahiko Naruko and Makiko Ueda and {Van der Wal}, {Allard C.} and {Van der Loos}, {Chris M.} and Hiroshi Itoh and Kazuwa Nakao and Becker, {Anton E.}",
year = "1996",
language = "English",
volume = "94",
pages = "3103--3108",
journal = "Circulation",
issn = "0009-7322",
publisher = "Lippincott Williams and Wilkins",
number = "12",

}

TY - JOUR

T1 - C-type natriuretic peptide in human coronary atherosclerotic lesions

AU - Naruko, Takahiko

AU - Ueda, Makiko

AU - Van der Wal, Allard C.

AU - Van der Loos, Chris M.

AU - Itoh, Hiroshi

AU - Nakao, Kazuwa

AU - Becker, Anton E.

PY - 1996

Y1 - 1996

N2 - Background: C-type natriuretic peptide (CNP) belongs to the natriuretic peptide family and is considered to have regulatory effects on vascular tone and smooth muscle growth. Since these features play a role in atherogenesis, the presence of CNP at such sites was studied. Methods and Results: Thirty- three coronary artery segments were harvested at autopsy: 10 normal, with diffuse intimal thickening, and 23 atherosclerotic lesions. Samples were snap-frozen and processed for immunohistochemical staining. For the identification of CNP, a mouse monoclonal antibody (KY-CNP-1) was used. 1A4, EBM-11 (CD68), and von Willebrand factor antibodies were used to stain smooth muscle cells, macrophages, and endothelial cells, respectively. CNP is present in several cell types. Normal arterial segments show CNP-positive endothelial cells. Hypercellular atherosclerotic lesions show distinct CNP positivity of smooth muscle cells and macrophages but a decrease in positivity of endothelial cells. Advanced atherosclerotic lesions contain CNP-positive macrophages, but the smooth muscle cells within the fibrous cap and the surface endothelial cells are almost all CNP negative. Conclusions: These observations suggest that CNP has functional significance in atherogenesis.

AB - Background: C-type natriuretic peptide (CNP) belongs to the natriuretic peptide family and is considered to have regulatory effects on vascular tone and smooth muscle growth. Since these features play a role in atherogenesis, the presence of CNP at such sites was studied. Methods and Results: Thirty- three coronary artery segments were harvested at autopsy: 10 normal, with diffuse intimal thickening, and 23 atherosclerotic lesions. Samples were snap-frozen and processed for immunohistochemical staining. For the identification of CNP, a mouse monoclonal antibody (KY-CNP-1) was used. 1A4, EBM-11 (CD68), and von Willebrand factor antibodies were used to stain smooth muscle cells, macrophages, and endothelial cells, respectively. CNP is present in several cell types. Normal arterial segments show CNP-positive endothelial cells. Hypercellular atherosclerotic lesions show distinct CNP positivity of smooth muscle cells and macrophages but a decrease in positivity of endothelial cells. Advanced atherosclerotic lesions contain CNP-positive macrophages, but the smooth muscle cells within the fibrous cap and the surface endothelial cells are almost all CNP negative. Conclusions: These observations suggest that CNP has functional significance in atherogenesis.

KW - atherosclerosis

KW - muscle, smooth

KW - natriuretic peptides

UR - http://www.scopus.com/inward/record.url?scp=0030452795&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030452795&partnerID=8YFLogxK

M3 - Article

C2 - 8989116

AN - SCOPUS:0030452795

VL - 94

SP - 3103

EP - 3108

JO - Circulation

JF - Circulation

SN - 0009-7322

IS - 12

ER -