C-type natriuretic peptide in human coronary atherosclerotic lesions

Takahiko Naruko, Makiko Ueda, Allard C. Van der Wal, Chris M. Van der Loos, Hiroshi Itoh, Kazuwa Nakao, Anton E. Becker

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Abstract

Background: C-type natriuretic peptide (CNP) belongs to the natriuretic peptide family and is considered to have regulatory effects on vascular tone and smooth muscle growth. Since these features play a role in atherogenesis, the presence of CNP at such sites was studied. Methods and Results: Thirty- three coronary artery segments were harvested at autopsy: 10 normal, with diffuse intimal thickening, and 23 atherosclerotic lesions. Samples were snap-frozen and processed for immunohistochemical staining. For the identification of CNP, a mouse monoclonal antibody (KY-CNP-1) was used. 1A4, EBM-11 (CD68), and von Willebrand factor antibodies were used to stain smooth muscle cells, macrophages, and endothelial cells, respectively. CNP is present in several cell types. Normal arterial segments show CNP-positive endothelial cells. Hypercellular atherosclerotic lesions show distinct CNP positivity of smooth muscle cells and macrophages but a decrease in positivity of endothelial cells. Advanced atherosclerotic lesions contain CNP-positive macrophages, but the smooth muscle cells within the fibrous cap and the surface endothelial cells are almost all CNP negative. Conclusions: These observations suggest that CNP has functional significance in atherogenesis.

Original languageEnglish
Pages (from-to)3103-3108
Number of pages6
JournalCirculation
Volume94
Issue number12
Publication statusPublished - 1996
Externally publishedYes

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Keywords

  • atherosclerosis
  • muscle, smooth
  • natriuretic peptides

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Naruko, T., Ueda, M., Van der Wal, A. C., Van der Loos, C. M., Itoh, H., Nakao, K., & Becker, A. E. (1996). C-type natriuretic peptide in human coronary atherosclerotic lesions. Circulation, 94(12), 3103-3108.