Caenorhabditis elegans anti-apoptotic gene ced-9 prevents ced-3-induced cell death in Drosophila cells

Shin Hisahara, Hirotaka Kanuka, Shin Ichi Shoji, Shingo Yoshikawa, Hideyuki Okano, Masayuki Miura

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

ced-9, a member of the bcl-2 gene family in Caenorhabditis elegans plays a central roles in preventing cell death in worms. Overexpression of human bcl-2 can partially prevent cell death in C. elegans. However, it remains to be elucidated whether ced-9 can regulate cell death when expressed in other organisms. We demonstrated that the CED-9 protein is co-localized with BCL-2 in COS cells and Drosophila Schneider's L2 (SL2) cells, suggesting that the site of CED-9 action is located to specific cytoplasmic compartments. Overexpression of ced-9 only poorly protected cells from the death induced by ced-3 in HeLa cells, but ced-9 significantly reduced the cell death induced by ced-3 in Drosophila SL2 cells. Furthermore, apoptosis of SL2 cells that was induced by a Drosophila cell-death gene, reaper, was shown to be partially prevented by ced-9, bcl-2 and bcl-xL. These results suggest that the signaling pathway that is required for the anti-apoptotic function of bcl-2 family members, including ced-9, is conserved in Drosophila cells. In addition, SL2 cells provide a unique systems for dissecting the main machinery of cell death.

Original languageEnglish
Pages (from-to)667-673
Number of pages7
JournalJournal of Cell Science
Volume111
Issue number6
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Caenorhabditis elegans
Drosophila
Cell Death
Genes
bcl-2 Genes
COS Cells
HeLa Cells
Apoptosis
Proteins

Keywords

  • Apoptosis
  • Caenorhabditis elegans
  • Caspase
  • ced-3
  • ced-9
  • Drosophila

ASJC Scopus subject areas

  • Cell Biology

Cite this

Hisahara, S., Kanuka, H., Shoji, S. I., Yoshikawa, S., Okano, H., & Miura, M. (1998). Caenorhabditis elegans anti-apoptotic gene ced-9 prevents ced-3-induced cell death in Drosophila cells. Journal of Cell Science, 111(6), 667-673.

Caenorhabditis elegans anti-apoptotic gene ced-9 prevents ced-3-induced cell death in Drosophila cells. / Hisahara, Shin; Kanuka, Hirotaka; Shoji, Shin Ichi; Yoshikawa, Shingo; Okano, Hideyuki; Miura, Masayuki.

In: Journal of Cell Science, Vol. 111, No. 6, 1998, p. 667-673.

Research output: Contribution to journalArticle

Hisahara, S, Kanuka, H, Shoji, SI, Yoshikawa, S, Okano, H & Miura, M 1998, 'Caenorhabditis elegans anti-apoptotic gene ced-9 prevents ced-3-induced cell death in Drosophila cells', Journal of Cell Science, vol. 111, no. 6, pp. 667-673.
Hisahara, Shin ; Kanuka, Hirotaka ; Shoji, Shin Ichi ; Yoshikawa, Shingo ; Okano, Hideyuki ; Miura, Masayuki. / Caenorhabditis elegans anti-apoptotic gene ced-9 prevents ced-3-induced cell death in Drosophila cells. In: Journal of Cell Science. 1998 ; Vol. 111, No. 6. pp. 667-673.
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