Caffeine sensitizes nondividing human fibroblasts to X rays by inducing a high frequency of misrepair

Tetsuya Kawata; Hisao Ito; Masayoshi Saito; Takashi Uno; Ryuichi Okayasu; Cuihua Liu; Momoe Kan'o; Kerry George; Francis A. Cucinotta

doi:10.1667/RR3382.1

Caffeine sensitizes nondividing human fibroblasts to X rays by inducing a high frequency of misrepair

Tetsuya Kawata, Hisao Ito, Masayoshi Saito, Takashi Uno, Ryuichi Okayasu, Cuihua Liu, Momoe Kan'o, Kerry George, Francis A. Cucinotta

Department of Radiology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Caffeine sensitizes cells to ionizing radiation, and this effect is believed to be associated with the disruption of DNA damage-responsive cell cycle checkpoints, which is controlled by ATM. Recent studies suggest that misrejoining of DSBs is one of the underlying mechanisms of AT cell hyper-radiosensitivity. In this study, we investigated the effects of caffeine and radiation on nongrowing G₀ normal human fibroblast cells by determining cell survival and scoring aberrations in calyculin A-induced G ₂ chromosomes. Results from the cell survival study indicate that after X-ray exposure G₀ cells were sensitized by 24 h treatment with caffeine. Analysis of chromosome aberrations using FISH (fluorescence in situ hybridization) revealed a high frequency of aberrant cells and color junctions in the caffeine-treated cells. Since most DNA repair in nongrowing G₀ cells is believed to result from nonhomologous end joining (NHEJ), caffeine may influence the fidelity of the NHEJ pathway in irradiated G₀ cells.

Original language	English
Pages (from-to)	509-513
Number of pages	5
Journal	Radiation Research
Volume	164
Issue number	4 II
DOIs	https://doi.org/10.1667/RR3382.1
Publication status	Published - 2005 Oct

ASJC Scopus subject areas

Biophysics
Radiation
Radiology Nuclear Medicine and imaging

Access to Document

10.1667/RR3382.1

Cite this

@article{42a257c208fe429ab17309dad2878c2e,

title = "Caffeine sensitizes nondividing human fibroblasts to X rays by inducing a high frequency of misrepair",

abstract = "Caffeine sensitizes cells to ionizing radiation, and this effect is believed to be associated with the disruption of DNA damage-responsive cell cycle checkpoints, which is controlled by ATM. Recent studies suggest that misrejoining of DSBs is one of the underlying mechanisms of AT cell hyper-radiosensitivity. In this study, we investigated the effects of caffeine and radiation on nongrowing G0 normal human fibroblast cells by determining cell survival and scoring aberrations in calyculin A-induced G 2 chromosomes. Results from the cell survival study indicate that after X-ray exposure G0 cells were sensitized by 24 h treatment with caffeine. Analysis of chromosome aberrations using FISH (fluorescence in situ hybridization) revealed a high frequency of aberrant cells and color junctions in the caffeine-treated cells. Since most DNA repair in nongrowing G0 cells is believed to result from nonhomologous end joining (NHEJ), caffeine may influence the fidelity of the NHEJ pathway in irradiated G0 cells.",

author = "Tetsuya Kawata and Hisao Ito and Masayoshi Saito and Takashi Uno and Ryuichi Okayasu and Cuihua Liu and Momoe Kan'o and Kerry George and Cucinotta, {Francis A.}",

year = "2005",

month = oct,

doi = "10.1667/RR3382.1",

language = "English",

volume = "164",

pages = "509--513",

journal = "Radiation Research",

issn = "0033-7587",

publisher = "Radiation Research Society",

number = "4 II",

}

TY - JOUR

T1 - Caffeine sensitizes nondividing human fibroblasts to X rays by inducing a high frequency of misrepair

AU - Kawata, Tetsuya

AU - Ito, Hisao

AU - Saito, Masayoshi

AU - Uno, Takashi

AU - Okayasu, Ryuichi

AU - Liu, Cuihua

AU - Kan'o, Momoe

AU - George, Kerry

AU - Cucinotta, Francis A.

PY - 2005/10

Y1 - 2005/10

N2 - Caffeine sensitizes cells to ionizing radiation, and this effect is believed to be associated with the disruption of DNA damage-responsive cell cycle checkpoints, which is controlled by ATM. Recent studies suggest that misrejoining of DSBs is one of the underlying mechanisms of AT cell hyper-radiosensitivity. In this study, we investigated the effects of caffeine and radiation on nongrowing G0 normal human fibroblast cells by determining cell survival and scoring aberrations in calyculin A-induced G 2 chromosomes. Results from the cell survival study indicate that after X-ray exposure G0 cells were sensitized by 24 h treatment with caffeine. Analysis of chromosome aberrations using FISH (fluorescence in situ hybridization) revealed a high frequency of aberrant cells and color junctions in the caffeine-treated cells. Since most DNA repair in nongrowing G0 cells is believed to result from nonhomologous end joining (NHEJ), caffeine may influence the fidelity of the NHEJ pathway in irradiated G0 cells.

AB - Caffeine sensitizes cells to ionizing radiation, and this effect is believed to be associated with the disruption of DNA damage-responsive cell cycle checkpoints, which is controlled by ATM. Recent studies suggest that misrejoining of DSBs is one of the underlying mechanisms of AT cell hyper-radiosensitivity. In this study, we investigated the effects of caffeine and radiation on nongrowing G0 normal human fibroblast cells by determining cell survival and scoring aberrations in calyculin A-induced G 2 chromosomes. Results from the cell survival study indicate that after X-ray exposure G0 cells were sensitized by 24 h treatment with caffeine. Analysis of chromosome aberrations using FISH (fluorescence in situ hybridization) revealed a high frequency of aberrant cells and color junctions in the caffeine-treated cells. Since most DNA repair in nongrowing G0 cells is believed to result from nonhomologous end joining (NHEJ), caffeine may influence the fidelity of the NHEJ pathway in irradiated G0 cells.

UR - http://www.scopus.com/inward/record.url?scp=25444532255&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25444532255&partnerID=8YFLogxK

U2 - 10.1667/RR3382.1

DO - 10.1667/RR3382.1

M3 - Article

C2 - 16187758

AN - SCOPUS:25444532255

SN - 0033-7587

VL - 164

SP - 509

EP - 513

JO - Radiation Research

JF - Radiation Research

IS - 4 II

ER -

Caffeine sensitizes nondividing human fibroblasts to X rays by inducing a high frequency of misrepair

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this