Calcium associated resistance to H2O2 in Chinese hamster V79 cells

Motohisa Kaneko, Fukiko Inoue, Taiko Oda

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To investigate whether the difference in cellular sensitivity of Chinese hamster V79 and their H2O2-resistant variant cells (Hpr-4) to H2O2 relates to the difference in intracellular Ca2+ concentration in these cells, we measured Ca2+ concentration by calcium ion analysis after loading these cells with Fura-2/AM. Intracellular Ca2+ concentration increased in both Chinese hamster V79 and Hpr-4 cells as extracellular Ca2+ concentration increased. However, the increase in intracellular Ca2+ concentration in response to extracellular H2O2 was more pronounced in Hpr- 4 than V79 cells. H2O2 cytotoxicity of Hpr-4 but not V79 cells was also decreased in response to the increase in extracellular Ca2+ concentration. In parallel with the decrease in cytotoxicity in response to increasing extracellular Ca2+ concentration, the frequency of mitochondrial DNA single strand breaks (SSB) in Hpr-4 cells also decreased without producing observable nuclear DNA SSB. Use of permeabilized V79 and Hpr-4 cells exposed to H2O2 showed that mitochondrial DNA SSB decreased when extramitochondrial Ca2+ concentration increased. These findings indicate that elevated intracellular Ca2+ concentration may protect against H2O2-induced mitochondrial damage and cytotoxicity in these cells. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)137-147
Number of pages11
JournalToxicology Letters
Volume115
Issue number2
DOIs
Publication statusPublished - 2000 May 19

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Cytotoxicity
Cricetulus
Calcium
Mitochondrial DNA
Fura-2
Ions
DNA
Single-Stranded DNA Breaks

Keywords

  • Ca
  • Chinese hamster V79
  • HO cytotoxicity
  • Mitochondrial DNA SSB

ASJC Scopus subject areas

  • Toxicology

Cite this

Calcium associated resistance to H2O2 in Chinese hamster V79 cells. / Kaneko, Motohisa; Inoue, Fukiko; Oda, Taiko.

In: Toxicology Letters, Vol. 115, No. 2, 19.05.2000, p. 137-147.

Research output: Contribution to journalArticle

Kaneko, Motohisa ; Inoue, Fukiko ; Oda, Taiko. / Calcium associated resistance to H2O2 in Chinese hamster V79 cells. In: Toxicology Letters. 2000 ; Vol. 115, No. 2. pp. 137-147.
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