Calcium regulates caveolin-1 expression at the transcriptional level

Xiao Yan Yang, Cheng Cheng Huang, Qi Ming Kan, Yan Li, Dan Liu, Xue Cheng Zhang, Toshinori Sato, Sadako Yamagata, Tatsuya Yamagata

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Caveolin-1, an indispensable component of caveolae serving as a transformation suppressor protein, is highly expressed in poorly metastatic mouse osteosarcoma FBJ-S1 cells while highly metastatic FBJ-LL cells express low levels of caveolin-1. Calcium concentration is higher in FBJ-S1 cells than in FBJ-LL cells; therefore, we investigated the possibility that calcium signaling positively regulates caveolin-1 in mouse FBJ-S1 cells. When cells were treated with the calcium channel blocker nifedipine, cyclosporin A (a calcineurin inhibitor), or INCA-6 (a nuclear factor of activated T-cells [NFAT] inhibitor), caveolin-1 expression at the mRNA and protein levels decreased. RNA silencing of voltage-dependent L-type calcium channel subunit alpha-1C resulted in suppression of caveolin-1 expression. This novel caveolin-1 regulation pathway was also identified in mouse NIH 3T3 cells and Lewis lung carcinoma cells. These results indicate that caveolin-1 is positively regulated at the transcriptional level through a novel calcium signaling pathway mediated by L-type calcium channel/Ca2+/calcineurin/NFAT.

Original languageEnglish
Pages (from-to)334-341
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume426
Issue number3
DOIs
Publication statusPublished - 2012 Sep 28

Fingerprint

Caveolin 1
Calcium
NFATC Transcription Factors
L-Type Calcium Channels
Calcium Signaling
Lewis Lung Carcinoma
Caveolae
NIH 3T3 Cells
Calcineurin
Calcium Channel Blockers
Osteosarcoma
Nifedipine
RNA Interference
Cyclosporine
Proteins
Cells
RNA
Messenger RNA

Keywords

  • Cacna1c
  • Calcium signaling
  • Caveolin-1
  • Cyclosporin A
  • FBJ osteosarcoma
  • L-type calcium ion channel

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Yang, X. Y., Huang, C. C., Kan, Q. M., Li, Y., Liu, D., Zhang, X. C., ... Yamagata, T. (2012). Calcium regulates caveolin-1 expression at the transcriptional level. Biochemical and Biophysical Research Communications, 426(3), 334-341. https://doi.org/10.1016/j.bbrc.2012.08.079

Calcium regulates caveolin-1 expression at the transcriptional level. / Yang, Xiao Yan; Huang, Cheng Cheng; Kan, Qi Ming; Li, Yan; Liu, Dan; Zhang, Xue Cheng; Sato, Toshinori; Yamagata, Sadako; Yamagata, Tatsuya.

In: Biochemical and Biophysical Research Communications, Vol. 426, No. 3, 28.09.2012, p. 334-341.

Research output: Contribution to journalArticle

Yang, XY, Huang, CC, Kan, QM, Li, Y, Liu, D, Zhang, XC, Sato, T, Yamagata, S & Yamagata, T 2012, 'Calcium regulates caveolin-1 expression at the transcriptional level', Biochemical and Biophysical Research Communications, vol. 426, no. 3, pp. 334-341. https://doi.org/10.1016/j.bbrc.2012.08.079
Yang, Xiao Yan ; Huang, Cheng Cheng ; Kan, Qi Ming ; Li, Yan ; Liu, Dan ; Zhang, Xue Cheng ; Sato, Toshinori ; Yamagata, Sadako ; Yamagata, Tatsuya. / Calcium regulates caveolin-1 expression at the transcriptional level. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 426, No. 3. pp. 334-341.
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