Calcium regulates caveolin-1 expression at the transcriptional level

Xiao Yan Yang; Cheng Cheng Huang; Qi Ming Kan; Yan Li; Dan Liu; Xue Cheng Zhang; Toshinori Sato; Sadako Yamagata; Tatsuya Yamagata

doi:10.1016/j.bbrc.2012.08.079

Calcium regulates caveolin-1 expression at the transcriptional level

Xiao Yan Yang, Cheng Cheng Huang, Qi Ming Kan, Yan Li, Dan Liu, Xue Cheng Zhang, Toshinori Sato, Sadako Yamagata, Tatsuya Yamagata

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Caveolin-1, an indispensable component of caveolae serving as a transformation suppressor protein, is highly expressed in poorly metastatic mouse osteosarcoma FBJ-S1 cells while highly metastatic FBJ-LL cells express low levels of caveolin-1. Calcium concentration is higher in FBJ-S1 cells than in FBJ-LL cells; therefore, we investigated the possibility that calcium signaling positively regulates caveolin-1 in mouse FBJ-S1 cells. When cells were treated with the calcium channel blocker nifedipine, cyclosporin A (a calcineurin inhibitor), or INCA-6 (a nuclear factor of activated T-cells [NFAT] inhibitor), caveolin-1 expression at the mRNA and protein levels decreased. RNA silencing of voltage-dependent L-type calcium channel subunit alpha-1C resulted in suppression of caveolin-1 expression. This novel caveolin-1 regulation pathway was also identified in mouse NIH 3T3 cells and Lewis lung carcinoma cells. These results indicate that caveolin-1 is positively regulated at the transcriptional level through a novel calcium signaling pathway mediated by L-type calcium channel/Ca²⁺/calcineurin/NFAT.

Original language	English
Pages (from-to)	334-341
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	426
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2012.08.079
Publication status	Published - 2012 Sept 28

Keywords

Cacna1c
Calcium signaling
Caveolin-1
Cyclosporin A
FBJ osteosarcoma
L-type calcium ion channel

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2012.08.079

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title = "Calcium regulates caveolin-1 expression at the transcriptional level",

abstract = "Caveolin-1, an indispensable component of caveolae serving as a transformation suppressor protein, is highly expressed in poorly metastatic mouse osteosarcoma FBJ-S1 cells while highly metastatic FBJ-LL cells express low levels of caveolin-1. Calcium concentration is higher in FBJ-S1 cells than in FBJ-LL cells; therefore, we investigated the possibility that calcium signaling positively regulates caveolin-1 in mouse FBJ-S1 cells. When cells were treated with the calcium channel blocker nifedipine, cyclosporin A (a calcineurin inhibitor), or INCA-6 (a nuclear factor of activated T-cells [NFAT] inhibitor), caveolin-1 expression at the mRNA and protein levels decreased. RNA silencing of voltage-dependent L-type calcium channel subunit alpha-1C resulted in suppression of caveolin-1 expression. This novel caveolin-1 regulation pathway was also identified in mouse NIH 3T3 cells and Lewis lung carcinoma cells. These results indicate that caveolin-1 is positively regulated at the transcriptional level through a novel calcium signaling pathway mediated by L-type calcium channel/Ca2+/calcineurin/NFAT.",

keywords = "Cacna1c, Calcium signaling, Caveolin-1, Cyclosporin A, FBJ osteosarcoma, L-type calcium ion channel",

author = "Yang, {Xiao Yan} and Huang, {Cheng Cheng} and Kan, {Qi Ming} and Yan Li and Dan Liu and Zhang, {Xue Cheng} and Toshinori Sato and Sadako Yamagata and Tatsuya Yamagata",

note = "Funding Information: This work was supported in part by funding from the Sadako Yamagata Memorial Foundation (to T.Y.). ",

year = "2012",

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AU - Yang, Xiao Yan

AU - Huang, Cheng Cheng

AU - Kan, Qi Ming

AU - Li, Yan

AU - Liu, Dan

AU - Zhang, Xue Cheng

AU - Sato, Toshinori

AU - Yamagata, Sadako

AU - Yamagata, Tatsuya

N1 - Funding Information: This work was supported in part by funding from the Sadako Yamagata Memorial Foundation (to T.Y.).

PY - 2012/9/28

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N2 - Caveolin-1, an indispensable component of caveolae serving as a transformation suppressor protein, is highly expressed in poorly metastatic mouse osteosarcoma FBJ-S1 cells while highly metastatic FBJ-LL cells express low levels of caveolin-1. Calcium concentration is higher in FBJ-S1 cells than in FBJ-LL cells; therefore, we investigated the possibility that calcium signaling positively regulates caveolin-1 in mouse FBJ-S1 cells. When cells were treated with the calcium channel blocker nifedipine, cyclosporin A (a calcineurin inhibitor), or INCA-6 (a nuclear factor of activated T-cells [NFAT] inhibitor), caveolin-1 expression at the mRNA and protein levels decreased. RNA silencing of voltage-dependent L-type calcium channel subunit alpha-1C resulted in suppression of caveolin-1 expression. This novel caveolin-1 regulation pathway was also identified in mouse NIH 3T3 cells and Lewis lung carcinoma cells. These results indicate that caveolin-1 is positively regulated at the transcriptional level through a novel calcium signaling pathway mediated by L-type calcium channel/Ca2+/calcineurin/NFAT.

AB - Caveolin-1, an indispensable component of caveolae serving as a transformation suppressor protein, is highly expressed in poorly metastatic mouse osteosarcoma FBJ-S1 cells while highly metastatic FBJ-LL cells express low levels of caveolin-1. Calcium concentration is higher in FBJ-S1 cells than in FBJ-LL cells; therefore, we investigated the possibility that calcium signaling positively regulates caveolin-1 in mouse FBJ-S1 cells. When cells were treated with the calcium channel blocker nifedipine, cyclosporin A (a calcineurin inhibitor), or INCA-6 (a nuclear factor of activated T-cells [NFAT] inhibitor), caveolin-1 expression at the mRNA and protein levels decreased. RNA silencing of voltage-dependent L-type calcium channel subunit alpha-1C resulted in suppression of caveolin-1 expression. This novel caveolin-1 regulation pathway was also identified in mouse NIH 3T3 cells and Lewis lung carcinoma cells. These results indicate that caveolin-1 is positively regulated at the transcriptional level through a novel calcium signaling pathway mediated by L-type calcium channel/Ca2+/calcineurin/NFAT.

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Calcium regulates caveolin-1 expression at the transcriptional level

Abstract

Keywords

ASJC Scopus subject areas

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