Calcium regulates caveolin-1 expression at the transcriptional level

Xiao Yan Yang, Cheng Cheng Huang, Qi Ming Kan, Yan Li, Dan Liu, Xue Cheng Zhang, Toshinori Sato, Sadako Yamagata, Tatsuya Yamagata

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Caveolin-1, an indispensable component of caveolae serving as a transformation suppressor protein, is highly expressed in poorly metastatic mouse osteosarcoma FBJ-S1 cells while highly metastatic FBJ-LL cells express low levels of caveolin-1. Calcium concentration is higher in FBJ-S1 cells than in FBJ-LL cells; therefore, we investigated the possibility that calcium signaling positively regulates caveolin-1 in mouse FBJ-S1 cells. When cells were treated with the calcium channel blocker nifedipine, cyclosporin A (a calcineurin inhibitor), or INCA-6 (a nuclear factor of activated T-cells [NFAT] inhibitor), caveolin-1 expression at the mRNA and protein levels decreased. RNA silencing of voltage-dependent L-type calcium channel subunit alpha-1C resulted in suppression of caveolin-1 expression. This novel caveolin-1 regulation pathway was also identified in mouse NIH 3T3 cells and Lewis lung carcinoma cells. These results indicate that caveolin-1 is positively regulated at the transcriptional level through a novel calcium signaling pathway mediated by L-type calcium channel/Ca2+/calcineurin/NFAT.

Original languageEnglish
Pages (from-to)334-341
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume426
Issue number3
DOIs
Publication statusPublished - 2012 Sep 28

Keywords

  • Cacna1c
  • Calcium signaling
  • Caveolin-1
  • Cyclosporin A
  • FBJ osteosarcoma
  • L-type calcium ion channel

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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