Calcium Transient Dynamics of Neural Ensembles in the Primary Motor Cortex of Naturally Behaving Monkeys

Takahiro Kondo, Risa Saito, Masaki Otaka, Kimika Yoshino-Saito, Akihiro Yamanaka, Tetsuo Yamamori, Akiya Watakabe, Hiroaki Mizukami, Mark J. Schnitzer, Kenji Tanaka, Junichi Ushiba, Hideyuki Okano

Research output: Contribution to journalArticle

Abstract

To understand brain circuits of cognitive behaviors under natural conditions, we developed techniques for imaging neuronal activities from large neuronal populations in the deep layer cortex of the naturally behaving common marmoset. Animals retrieved food pellets or climbed ladders as a miniature fluorescence microscope monitored hundreds of calcium indicator-expressing cortical neurons in the right primary motor cortex. This technique, which can be adapted to other brain regions, can deepen our understanding of brain circuits by facilitating longitudinal population analyses of neuronal representation associated with cognitive naturalistic behaviors and their pathophysiological processes. Kondo et al. demonstrate calcium imaging from neuronal populations using a miniature fluorescence microscope in naturally behaving non-human primates (NHPs), common marmosets. This technique marks an advance beyond methods that use fixed head positioning, which limits the study of complex, self-determined behaviors such as social interactions or fear and anxiety.

Original languageEnglish
Pages (from-to)2191-2195.e4
JournalCell Reports
Volume24
Issue number8
DOIs
Publication statusPublished - 2018 Aug 21

Fingerprint

Motor Cortex
Haplorhini
Callithrix
Brain
Calcium
Microscopes
Fluorescence
Population
Imaging techniques
Networks (circuits)
Ladders
Interpersonal Relations
Primates
Neurons
Fear
Animals
Anxiety
Head
Food

Keywords

  • calcium imaging
  • common marmoset
  • miniaturized microscope
  • preferred direction
  • primary motor cortex

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Calcium Transient Dynamics of Neural Ensembles in the Primary Motor Cortex of Naturally Behaving Monkeys. / Kondo, Takahiro; Saito, Risa; Otaka, Masaki; Yoshino-Saito, Kimika; Yamanaka, Akihiro; Yamamori, Tetsuo; Watakabe, Akiya; Mizukami, Hiroaki; Schnitzer, Mark J.; Tanaka, Kenji; Ushiba, Junichi; Okano, Hideyuki.

In: Cell Reports, Vol. 24, No. 8, 21.08.2018, p. 2191-2195.e4.

Research output: Contribution to journalArticle

Kondo, T, Saito, R, Otaka, M, Yoshino-Saito, K, Yamanaka, A, Yamamori, T, Watakabe, A, Mizukami, H, Schnitzer, MJ, Tanaka, K, Ushiba, J & Okano, H 2018, 'Calcium Transient Dynamics of Neural Ensembles in the Primary Motor Cortex of Naturally Behaving Monkeys', Cell Reports, vol. 24, no. 8, pp. 2191-2195.e4. https://doi.org/10.1016/j.celrep.2018.07.057
Kondo T, Saito R, Otaka M, Yoshino-Saito K, Yamanaka A, Yamamori T et al. Calcium Transient Dynamics of Neural Ensembles in the Primary Motor Cortex of Naturally Behaving Monkeys. Cell Reports. 2018 Aug 21;24(8):2191-2195.e4. https://doi.org/10.1016/j.celrep.2018.07.057
Kondo, Takahiro ; Saito, Risa ; Otaka, Masaki ; Yoshino-Saito, Kimika ; Yamanaka, Akihiro ; Yamamori, Tetsuo ; Watakabe, Akiya ; Mizukami, Hiroaki ; Schnitzer, Mark J. ; Tanaka, Kenji ; Ushiba, Junichi ; Okano, Hideyuki. / Calcium Transient Dynamics of Neural Ensembles in the Primary Motor Cortex of Naturally Behaving Monkeys. In: Cell Reports. 2018 ; Vol. 24, No. 8. pp. 2191-2195.e4.
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