Calcium waves along the cleavage furrows in cleavage-stage Xenopus embryos and its inhibition by heparin

Akira Muto, Shoen Kume, Takafumi Inoue, Hideyuki Okano, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Calcium signaling is known to be associated with cytokinesis; however, the detailed spatio-temporal pattern of calcium dynamics has remained unclear. We have studied changes of intracellular free calcium in cleavage- stage Xenopus embryos using fluorescent calcium indictor dyes, mainly Calcium Green-1. Cleavage formation was followed by calcium transients that localized to cleavage furrows and propagated along the furrows as calcium waves. The calcium transients at the cleavage furrows were observed at each cleavage furrow at least until blastula stage. The velocity of the calcium waves at the first cleavage furrow was ~3 μ/s, which was much slower than that associated with fertilization/egg activation. These calcium waves traveled only along the cleavage furrows and not in the direction orthogonal to the furrows. These observations imply that there exists an intracellular calcium releasing activity specifically associated with cleavage furrows. The calcium waves occurred in the absence of extracellular calcium and were inhibited in embryos injected with heparin, an inositol 1,4,5-trisphosphate (InsP3) receptor antagonist. These results suggest that InsP3 receptor mediated calcium mobilization plays an essential role in calcium wave formation at the cleavage furrows.

Original languageEnglish
Pages (from-to)181-190
Number of pages10
JournalJournal of Cell Biology
Volume135
Issue number1
DOIs
Publication statusPublished - 1996 Oct
Externally publishedYes

Fingerprint

Calcium Signaling
Xenopus
Heparin
Embryonic Structures
Calcium
Blastula
Calcium-Sensing Receptors
Inositol 1,4,5-Trisphosphate Receptors
Cytokinesis
Fertilization
Ovum
Coloring Agents

ASJC Scopus subject areas

  • Cell Biology

Cite this

Calcium waves along the cleavage furrows in cleavage-stage Xenopus embryos and its inhibition by heparin. / Muto, Akira; Kume, Shoen; Inoue, Takafumi; Okano, Hideyuki; Mikoshiba, Katsuhiko.

In: Journal of Cell Biology, Vol. 135, No. 1, 10.1996, p. 181-190.

Research output: Contribution to journalArticle

Muto, Akira ; Kume, Shoen ; Inoue, Takafumi ; Okano, Hideyuki ; Mikoshiba, Katsuhiko. / Calcium waves along the cleavage furrows in cleavage-stage Xenopus embryos and its inhibition by heparin. In: Journal of Cell Biology. 1996 ; Vol. 135, No. 1. pp. 181-190.
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