Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?

Yukiji Takeda, Taisuke Mori, Hideaki Imabayashi, Tohru Kiyono, Satoshi Gojo, Shunichirou Miyoshi, Naoko Hida, Makoto Ita, Kaoru Segawa, Satoshi Ogawa, Michiie Sakamoto, Shinobu Nakamura, Akihiro Umezawa

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Abstract

Background. Cell transplantation has recently been challenged to improve cardiac function of severe heart failure. Human mesenchymal stem cells (hMSCs) are multipotent cells that can be isolated from adult marrow stroma, but because of their limited life span, it is difficult to study them further. To overcome this problem, we attempted to prolong the life span of hMSCs and investigate whether the hMSCs modified with cell-cycle-associated genes can differentiate into cardiomyocytes in vitro. Methods. We attempted to prolong the life span of hMSCs by infecting retrovirus encoding bmi-1, human papillomavirus E6 and E7, and/or human telomerase reverse transcriptase genes. To determine whether the hMSCs with an extended life span could differentiate into cardiomyocytes, 5-azacytidine-treated hMSCs were co-cultured with fetal cardiomyocytes in vitro. Result. The established hMSCs proliferated over 150 population doublings. On day 3 of co-cultivation, the hMSCs became elongated, like myotubes, began spontaneously beating, and acquired automaticity. Their rhythm clearly differed from that of the surrounding fetal mouse cardiomyocytes. The number of beating cardiomyocytes increased until 3 weeks. hMSCs clearly exhibited differentiated cardiomyocyte phenotypes in vitro as revealed by immunocytochemistry, RT-PCR, and action potential recording. Conclusions. The life span of hMSCs was prolonged without interfering with cardiomyogenic differentiation. hMSCs with an extended life span can be used to produce a good experimental model of cardiac cell transplantation and may serve as a highly useful cell source for cardiomyocytic transplantation.

Original languageEnglish
Pages (from-to)833-845
Number of pages13
JournalJournal of Gene Medicine
Volume6
Issue number8
DOIs
Publication statusPublished - 2004 Aug 1

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Keywords

  • Bmi-1
  • Cardiomyocytes
  • Immortalization
  • Marrow stroma
  • Papillomavirus
  • Senescence

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Drug Discovery
  • Genetics(clinical)

Cite this

Takeda, Y., Mori, T., Imabayashi, H., Kiyono, T., Gojo, S., Miyoshi, S., Hida, N., Ita, M., Segawa, K., Ogawa, S., Sakamoto, M., Nakamura, S., & Umezawa, A. (2004). Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation? Journal of Gene Medicine, 6(8), 833-845. https://doi.org/10.1002/jgm.583