Candidate markers for stem and early progenitor cells, Musashi-1 and Hes1, are expressed in crypt base columnar cells of mouse small intestine

Takahisa Kayahara, Mitsutaka Sawada, Shigeo Takaishi, Hirokazu Fukui, Hiroshi Seno, Hiroaki Fukuzawa, Katsumasa Suzuki, Hiroshi Hiai, Ryoichiro Kageyama, Hideyuki Okano, Tsutomu Chiba

Research output: Contribution to journalArticle

251 Citations (Scopus)

Abstract

Musashi-1, a neural RNA-binding protein, is important for maintaining neural stem cells. Both Musashi-1 and Hes1, a transcriptional factor regulated by Musashi-1, are expressed in the small intestine. Here we show that Musashi-1 is present in a few epithelial cells just above the Paneth cells in the small intestinal crypt, the putative position of stem cells, whereas Hes1 is expressed in lower crypt cells just above the Paneth cells, including Musashi-1-positive cells. Musashi-1 and Hes1 were not expressed in Paneth cells. Notably, Musashi-1 and Hes1 were coexpressed in the crypt base columnar cells located between the Paneth cells. These findings suggest that not only the cells just above Paneth cells but also the crypt base columnar cells between the Paneth cells have stem cell characteristics.

Original languageEnglish
Pages (from-to)131-135
Number of pages5
JournalFEBS Letters
Volume535
Issue number1-3
DOIs
Publication statusPublished - 2003 Jan 30

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Paneth Cells
Stem cells
Small Intestine
Stem Cells
Cells
RNA-Binding Proteins
Neural Stem Cells
Epithelial Cells

Keywords

  • Crypt base columnar cell
  • Hes1
  • Musashi-1
  • Small intestine
  • Stem cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Candidate markers for stem and early progenitor cells, Musashi-1 and Hes1, are expressed in crypt base columnar cells of mouse small intestine. / Kayahara, Takahisa; Sawada, Mitsutaka; Takaishi, Shigeo; Fukui, Hirokazu; Seno, Hiroshi; Fukuzawa, Hiroaki; Suzuki, Katsumasa; Hiai, Hiroshi; Kageyama, Ryoichiro; Okano, Hideyuki; Chiba, Tsutomu.

In: FEBS Letters, Vol. 535, No. 1-3, 30.01.2003, p. 131-135.

Research output: Contribution to journalArticle

Kayahara, T, Sawada, M, Takaishi, S, Fukui, H, Seno, H, Fukuzawa, H, Suzuki, K, Hiai, H, Kageyama, R, Okano, H & Chiba, T 2003, 'Candidate markers for stem and early progenitor cells, Musashi-1 and Hes1, are expressed in crypt base columnar cells of mouse small intestine', FEBS Letters, vol. 535, no. 1-3, pp. 131-135. https://doi.org/10.1016/S0014-5793(02)03896-6
Kayahara, Takahisa ; Sawada, Mitsutaka ; Takaishi, Shigeo ; Fukui, Hirokazu ; Seno, Hiroshi ; Fukuzawa, Hiroaki ; Suzuki, Katsumasa ; Hiai, Hiroshi ; Kageyama, Ryoichiro ; Okano, Hideyuki ; Chiba, Tsutomu. / Candidate markers for stem and early progenitor cells, Musashi-1 and Hes1, are expressed in crypt base columnar cells of mouse small intestine. In: FEBS Letters. 2003 ; Vol. 535, No. 1-3. pp. 131-135.
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