Capillary Electromigration Techniques in Metabolomics

Akiyoshi Hirayama, Tomoyoshi Soga

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter provides an overview of the application of capillary electromigration techniques, particularly capillary electrophoresis-mass spectrometry (CE MS), in metabolomics. Compared with other metabolomics platforms, CE MS offers a number of advantages, including high sensitivity and ability to separate most charged metabolites. Therefore, it is well suited for the analysis of intermediate metabolites in energy metabolism, such as glycolysis, pentose phosphate pathway and tricarboxylic acid (TCA) cycle, which are mostly negatively charged. First the technical aspects of CE MS are covered, including CE separation modes, MS types, interface and microfluidics systems and data analysis strategies. Subsequently, applications of CE MS in targeted and non targeted metabolomics are described in detail.

Original languageEnglish
Title of host publicationChromatographic Methods in Metabolomics
EditorsSusanne Wiedmer, Tuulia Hyotylainen
PublisherRoyal Society of Chemistry
Pages114-137
Number of pages24
Edition18
DOIs
Publication statusPublished - 2013 Jan 1

Publication series

NameRSC Chromatography Monographs
Number18
Volume2013-January
ISSN (Print)1757-7055
ISSN (Electronic)1757-7063

Fingerprint

Capillary electrophoresis
Metabolomics
Electromigration
Capillary Electrophoresis
Mass spectrometry
Mass Spectrometry
Metabolites
Pentoses
Microfluidic Analytical Techniques
Pentose Phosphate Pathway
Citric Acid Cycle
Glycolysis
Systems Analysis
Microfluidics
Energy Metabolism
Phosphates
Systems analysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Chemical Engineering (miscellaneous)

Cite this

Hirayama, A., & Soga, T. (2013). Capillary Electromigration Techniques in Metabolomics. In S. Wiedmer, & T. Hyotylainen (Eds.), Chromatographic Methods in Metabolomics (18 ed., pp. 114-137). (RSC Chromatography Monographs; Vol. 2013-January, No. 18). Royal Society of Chemistry. https://doi.org/10.1039/9781849737272-00114

Capillary Electromigration Techniques in Metabolomics. / Hirayama, Akiyoshi; Soga, Tomoyoshi.

Chromatographic Methods in Metabolomics. ed. / Susanne Wiedmer; Tuulia Hyotylainen. 18. ed. Royal Society of Chemistry, 2013. p. 114-137 (RSC Chromatography Monographs; Vol. 2013-January, No. 18).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hirayama, A & Soga, T 2013, Capillary Electromigration Techniques in Metabolomics. in S Wiedmer & T Hyotylainen (eds), Chromatographic Methods in Metabolomics. 18 edn, RSC Chromatography Monographs, no. 18, vol. 2013-January, Royal Society of Chemistry, pp. 114-137. https://doi.org/10.1039/9781849737272-00114
Hirayama A, Soga T. Capillary Electromigration Techniques in Metabolomics. In Wiedmer S, Hyotylainen T, editors, Chromatographic Methods in Metabolomics. 18 ed. Royal Society of Chemistry. 2013. p. 114-137. (RSC Chromatography Monographs; 18). https://doi.org/10.1039/9781849737272-00114
Hirayama, Akiyoshi ; Soga, Tomoyoshi. / Capillary Electromigration Techniques in Metabolomics. Chromatographic Methods in Metabolomics. editor / Susanne Wiedmer ; Tuulia Hyotylainen. 18. ed. Royal Society of Chemistry, 2013. pp. 114-137 (RSC Chromatography Monographs; 18).
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