Capillary Electromigration Techniques in Metabolomics

Akiyoshi Hirayama, Tomoyoshi Soga

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter provides an overview of the application of capillary electromigration techniques, particularly capillary electrophoresis-mass spectrometry (CE MS), in metabolomics. Compared with other metabolomics platforms, CE MS offers a number of advantages, including high sensitivity and ability to separate most charged metabolites. Therefore, it is well suited for the analysis of intermediate metabolites in energy metabolism, such as glycolysis, pentose phosphate pathway and tricarboxylic acid (TCA) cycle, which are mostly negatively charged. First the technical aspects of CE MS are covered, including CE separation modes, MS types, interface and microfluidics systems and data analysis strategies. Subsequently, applications of CE MS in targeted and non targeted metabolomics are described in detail.

Original languageEnglish
Title of host publicationChromatographic Methods in Metabolomics
EditorsTuulia Hyotylainen, Susanne Wiedmer
PublisherRoyal Society of Chemistry
Pages114-137
Number of pages24
Edition18
ISBN (Print)9781849736077
DOIs
Publication statusPublished - 2013 Jan 1

Publication series

NameRSC Chromatography Monographs
Number18
Volume2013-January
ISSN (Print)1757-7055
ISSN (Electronic)1757-7063

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Chemical Engineering (miscellaneous)

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  • Cite this

    Hirayama, A., & Soga, T. (2013). Capillary Electromigration Techniques in Metabolomics. In T. Hyotylainen, & S. Wiedmer (Eds.), Chromatographic Methods in Metabolomics (18 ed., pp. 114-137). (RSC Chromatography Monographs; Vol. 2013-January, No. 18). Royal Society of Chemistry. https://doi.org/10.1039/9781849737272-00114