Capillary electrophoresis-mass spectrometry

Masataka Wakayama; Akiyoshi Hirayama; Tomoyoshi Soga

doi:10.1007/978-1-4939-2377-9_9

Capillary electrophoresis-mass spectrometry

Masataka Wakayama, Akiyoshi Hirayama, Tomoyoshi Soga

Graduate School of Media and Governance

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.

Original language	English
Pages (from-to)	113-122
Number of pages	10
Journal	Methods in Molecular Biology
Volume	1277
DOIs	https://doi.org/10.1007/978-1-4939-2377-9_9
Publication status	Published - 2015

Keywords

Amino acids
Capillary electrophoresis
Carboxylic acids
Mass spectrometry
Metabolome
Metabolomics
Sugar phosphates

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-2377-9_9

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title = "Capillary electrophoresis-mass spectrometry",

abstract = "Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.",

keywords = "Amino acids, Capillary electrophoresis, Carboxylic acids, Mass spectrometry, Metabolome, Metabolomics, Sugar phosphates",

author = "Masataka Wakayama and Akiyoshi Hirayama and Tomoyoshi Soga",

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doi = "10.1007/978-1-4939-2377-9_9",

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AU - Wakayama, Masataka

AU - Hirayama, Akiyoshi

AU - Soga, Tomoyoshi

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PY - 2015

Y1 - 2015

N2 - Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.

AB - Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.

KW - Amino acids

KW - Capillary electrophoresis

KW - Carboxylic acids

KW - Mass spectrometry

KW - Metabolome

KW - Metabolomics

KW - Sugar phosphates

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

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Capillary electrophoresis-mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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