Capillary electrophoresis-mass spectrometry-based metabolome analysis of serum and saliva from neurodegenerative dementia patients

Mayuko Tsuruoka, Junko Hara, Akiyoshi Hirayama, Masahiro Sugimoto, Tomoyoshi Soga, William R. Shankle, Masaru Tomita

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Despite increasing global prevalence, the precise pathogenesis and terms for objective diagnosis of neurodegenerative dementias remain controversial, and comprehensive understanding of the disease remains lacking. Here, we conducted metabolomic analysis of serum and saliva obtained from patients with neurodegenerative dementias (n = 10), including Alzheimer's disease, frontotemporal lobe dementia, and Lewy body disease, as well as from age-matched healthy controls (n = 9). Using CE-TOF-MS, six metabolites in serum (β-alanine, creatinine, hydroxyproline, glutamine, iso-citrate, and cytidine) and two in saliva (arginine and tyrosine) were significantly different between dementias and controls. Using multivariate analysis, serum was confirmed as a more efficient biological fluid for diagnosis compared to saliva; additionally, 45 metabolites in total were identified as candidate markers that could discriminate at least one pair of diagnostic groups from the healthy control group. These metabolites possibly provide an objective method for diagnosing dementia-type by multiphase screening. Moreover, diagnostic-type-dependent differences were observed in several tricarboxylic acid cycle compounds detected in serum, indicating that some pathways in glucose metabolism may be altered in dementia patients. This pilot study revealed novel alterations in metabolomic profiles between various neurodegenerative dementias, which would contribute to etiological investigations.

Original languageEnglish
Pages (from-to)2865-2872
Number of pages8
JournalElectrophoresis
Volume34
Issue number19
DOIs
Publication statusPublished - 2013 Oct

Fingerprint

Capillary electrophoresis
Metabolome
Capillary Electrophoresis
Metabolites
Saliva
Mass spectrometry
Dementia
Mass Spectrometry
Serum
Cytidine
Metabolomics
Hydroxyproline
Glutamine
Metabolism
Citric Acid
Alanine
Tyrosine
Arginine
Creatinine
Screening

Keywords

  • Alzheimer's disease
  • Capillary electrophoresis-mass spectrometry
  • Frontotemporal lobe degeneration
  • Lewy body disease
  • Metabolome

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Capillary electrophoresis-mass spectrometry-based metabolome analysis of serum and saliva from neurodegenerative dementia patients. / Tsuruoka, Mayuko; Hara, Junko; Hirayama, Akiyoshi; Sugimoto, Masahiro; Soga, Tomoyoshi; Shankle, William R.; Tomita, Masaru.

In: Electrophoresis, Vol. 34, No. 19, 10.2013, p. 2865-2872.

Research output: Contribution to journalArticle

Tsuruoka, Mayuko ; Hara, Junko ; Hirayama, Akiyoshi ; Sugimoto, Masahiro ; Soga, Tomoyoshi ; Shankle, William R. ; Tomita, Masaru. / Capillary electrophoresis-mass spectrometry-based metabolome analysis of serum and saliva from neurodegenerative dementia patients. In: Electrophoresis. 2013 ; Vol. 34, No. 19. pp. 2865-2872.
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