Capillary electrophoresis method for the analysis of inorganic anions, organic acids, amino acids, nucleotides, carbohydrates and other anionic compounds

Tomoyoshi Soga, Motohiro Imaizumi

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

A previously developed capillary zone electrophoresis (CZE) method with indirect UV detection for the simultaneous determination of inorganic and organic anions, amino acids and carbohydrates using 20 mM 2,6-pyridinedicarboxylic acid (PDC) as the background electrolyte was extended to allow determination of 206 anions including those above - mentioned and physiological amino acids, nucleotides, aromatic acids, haloacetic acids, alcohols, phosphorylated saccharides, oxyhalides, metal oxoacids, metal-ethylenediaminetetraacetic acid (EDTA) complexes, forensic anions, Good's buffers and herbicides. Every compound could be analyzed and their electrophoretic mobility determined simply by selecting detection wavelength. This method is simple and universal for anion analysis, and could be readily applied to the simultaneous determination of anionic compounds. In this work, it was used to identify and quantify important anions in sea urchin and sake.

Original languageEnglish
Pages (from-to)3418-3425
Number of pages8
JournalElectrophoresis
Volume22
Issue number16
DOIs
Publication statusPublished - 2001

Fingerprint

Capillary electrophoresis
Organic acids
Capillary Electrophoresis
Anions
Nucleotides
Carbohydrates
Amino Acids
Acids
Metals
Keto Acids
Aromatic Amino Acids
Electrophoretic mobility
Sea Urchins
Herbicides
Electrophoresis
Carboxylic acids
Edetic Acid
Electrolytes
Buffers
Alcohols

Keywords

  • Amino acids
  • Anions
  • Capillary electrophoresis
  • Carbohydrates
  • Nucleotides

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

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KW - Nucleotides

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