Carbohydrate epitopes and mucins expressed by 17 human ovarian carcinoma cell lines

Yutaka Tamada, Shin Ichiro Iida, Daisuke Aoki, Shiro Nozawa, Tatsuro Irimura

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Ovarian carcinomas are known to be diverse in their histological types and response to therapeutic agents. Specific immunotherapy by the use of mucins or carbohydrate vaccines has been attempted, in which expression of these epitopes by each histotype should clearly be assessed. Seventeen human ovarian carcinoma cell lines were evaluated for their expression of mucin core polypeptide mRNAs (MUC1, MUC2, MUC3, MUC5AC, MUC5B, and MUC6) by the reverse transcription-polymerase chain reaction. They were also examined for their expression of mucin-associated carbohydrate epitopes by flow cytometry. .Eleven monoclonal antibodies, including those specific for Lewis antigen-related epitopes and Tn-related epitopes, were used. Expression of the MUC1 gene and sialyl Lewis X was characteristic to all five cell lines derived from clear cell adenocarcinomas. Multiple mucin genes and a diverse range of carbohydrate epitopes were observed with all six cell lines derived from mucinous adenocarcinomas. Mucin-associated carbohydrate epitopes were not detected on three of four serous adenocarcinoma cell lines, although MUC1 and MUC2 mRNAs were detected in all of them. Therefore, ovarian carcinoma cells from tumors of different histological types showed characteristic expression patterns of mucin genes and carbohydrate epitopes.

Original languageEnglish
Pages (from-to)233-241
Number of pages9
JournalOncology Research
Volume11
Issue number5
Publication statusPublished - 1999

Fingerprint

Mucins
Epitopes
Carbohydrates
Carcinoma
Cell Line
Clear Cell Adenocarcinoma
Mucinous Adenocarcinoma
Messenger RNA
Immunotherapy
Genes
Reverse Transcription
Flow Cytometry
Adenocarcinoma
Vaccines
Monoclonal Antibodies
Gene Expression
Antigens
Polymerase Chain Reaction
Peptides
Neoplasms

Keywords

  • Carbohydrate epitope
  • Monoclonal antibody
  • Mucins
  • Ovarian carcinoma
  • Prognosis
  • Reverse transcription-polymerase chain reaction

ASJC Scopus subject areas

  • Cancer Research

Cite this

Tamada, Y., Iida, S. I., Aoki, D., Nozawa, S., & Irimura, T. (1999). Carbohydrate epitopes and mucins expressed by 17 human ovarian carcinoma cell lines. Oncology Research, 11(5), 233-241.

Carbohydrate epitopes and mucins expressed by 17 human ovarian carcinoma cell lines. / Tamada, Yutaka; Iida, Shin Ichiro; Aoki, Daisuke; Nozawa, Shiro; Irimura, Tatsuro.

In: Oncology Research, Vol. 11, No. 5, 1999, p. 233-241.

Research output: Contribution to journalArticle

Tamada, Y, Iida, SI, Aoki, D, Nozawa, S & Irimura, T 1999, 'Carbohydrate epitopes and mucins expressed by 17 human ovarian carcinoma cell lines', Oncology Research, vol. 11, no. 5, pp. 233-241.
Tamada, Yutaka ; Iida, Shin Ichiro ; Aoki, Daisuke ; Nozawa, Shiro ; Irimura, Tatsuro. / Carbohydrate epitopes and mucins expressed by 17 human ovarian carcinoma cell lines. In: Oncology Research. 1999 ; Vol. 11, No. 5. pp. 233-241.
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