Carbon monoxide modulates endotoxin-induced microvascular leukocyte adhesion through platelet-dependent mechanisms

Hiroshi Morisaki, Tomihiro Katayama, Yoshifumi Kotake, Masaharu Ito, Makoto Handa, Yasuo Ikeda, Junzo Takeda, Makoto Suematsu

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background: Although precise mechanisms remain to be determined, recent studies show that heme oxygenase-1 (HO-1), providing endogenous carbon monoxide (CO) and bilirubin, serves as an antiinflammatory enzyme. This study aimed to clarify roles of CO in regulation of microvascular adhesion of platelets and leukocytes in endotoxemia. Methods: Rats pretreated with or without hemin were anesthetized with pentobarbital and received continuous infusion of endotoxin. Platelets labeled with carboxyfluorescein diacetate succinimidyl ester and leukocyte behavior in mesenteric venules were visualized using intravital ultra-high-speed intensified fluorescence videomicroscopy. To examine the mechanisms for the effects of HO-1 on platelet and leukocyte behavior during endotoxemia, these studies were repeated with superfusion of either CO, bilirubin, or zinc protoporphyrine-IX. Results: Endotoxin caused a marked depression of platelet velocity traversing along periendothelial regions, accompanied by augmented rolling and adhesion of leukocytes in venules. The endotoxin-elicited changes were attenuated by the HO-1 induction with hemin and restored by blockade of the enzyme activity with zinc protoporphyrine-IX, a potent inhibitor of HO-1. Such an inhibitory action of HO-1 on microvascular cell adhesion was reproduced by local superfusion of the buffer containing CO at micromolar concentrations. Such antiadhesive actions of CO on leukocytes disappeared under immunoneutralization of glycoprotein Ibα, an adhesion molecule against platelets, but not against leukocytes. Platelets isolated from hemin-treated rats increased their ability to generate CO and displayed lesser sensitivity of agonist-induced aggregation than those from controls. Conclusions: These results suggest that CO desensitizes endotoxin-induced adhesive responses of leukocytes, mainly through its ability to ameliorate platelet activation in vivo.

Original languageEnglish
Pages (from-to)701-709
Number of pages9
JournalAnesthesiology
Volume97
Issue number3
DOIs
Publication statusPublished - 2002 Sep

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Carbon Monoxide
Endotoxins
Heme Oxygenase-1
Leukocytes
Blood Platelets
Hemin
Aptitude
Endotoxemia
Venules
Bilirubin
Zinc
Leukocyte Rolling
Platelet Glycoprotein GPIb-IX Complex
Video Microscopy
Platelet Activation
Pentobarbital
Enzymes
Cell Adhesion
Adhesives
Buffers

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Carbon monoxide modulates endotoxin-induced microvascular leukocyte adhesion through platelet-dependent mechanisms. / Morisaki, Hiroshi; Katayama, Tomihiro; Kotake, Yoshifumi; Ito, Masaharu; Handa, Makoto; Ikeda, Yasuo; Takeda, Junzo; Suematsu, Makoto.

In: Anesthesiology, Vol. 97, No. 3, 09.2002, p. 701-709.

Research output: Contribution to journalArticle

Morisaki, Hiroshi ; Katayama, Tomihiro ; Kotake, Yoshifumi ; Ito, Masaharu ; Handa, Makoto ; Ikeda, Yasuo ; Takeda, Junzo ; Suematsu, Makoto. / Carbon monoxide modulates endotoxin-induced microvascular leukocyte adhesion through platelet-dependent mechanisms. In: Anesthesiology. 2002 ; Vol. 97, No. 3. pp. 701-709.
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