Cartridge Device Capable of Spontaneous Solution Exchange for Sensor Cell Production

Aoi Tanaka; Sho Takamori; Hisatoshi Mimura; Toshihisa Osaki; Norihisa Miki; Shoji Takeuchi

doi:10.1109/MEMS51670.2022.9699570

Cartridge Device Capable of Spontaneous Solution Exchange for Sensor Cell Production

Aoi Tanaka, Sho Takamori, Hisatoshi Mimura, Toshihisa Osaki, Norihisa Miki, Shoji Takeuchi

Department of Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This paper reports a cartridge-type device to produce sensor cells with spontaneous solution exchange. Our group has developed a cell-based odorant sensor expressed olfactory receptors. These sensor cells presented excellent sensitivity and specificity, which would be useful for a wide range of sensor applications. However, production of such sensor cells requires multiple times of aseptic solution exchanges. In this work, we develop a cartridge-type cell culture device that utilizes capillary force for spontaneous solution exchange without pumps or tubes. When the cartridge is set to the exchange stage, the specified volume of solution is exchanged immediately. We experimentally examine the solution exchange, and demonstrate the response of a sensor cell to a chemical stimulus in the exchanged solution. We consider that the device would be useful to produce multiple types of sensor cells.

Original language	English
Title of host publication	35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
Publisher	IEEE Computer Society
Pages	166-167
Number of pages	2
ISBN (Electronic)	9781665409117
DOIs	https://doi.org/10.1109/MEMS51670.2022.9699570
Publication status	Published - 2022
Event	35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 - Tokyo, Japan Duration: 2022 Jan 9 → 2022 Jan 13

Publication series

Name	IEEE Symposium on Mass Storage Systems and Technologies
Volume	2022-January
ISSN (Print)	2160-1968

Conference

Conference	35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
Country/Territory	Japan
City	Tokyo
Period	22/1/9 → 22/1/13

Keywords

Biohybrid odorant sensor
Capillary force
Insect olfactory receptor
Sensor cell
Solution exchange

ASJC Scopus subject areas

Hardware and Architecture
Electrical and Electronic Engineering

Access to Document

10.1109/MEMS51670.2022.9699570

Cite this

Tanaka, A., Takamori, S., Mimura, H., Osaki, T., Miki, N., & Takeuchi, S. (2022). Cartridge Device Capable of Spontaneous Solution Exchange for Sensor Cell Production. In 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 (pp. 166-167). (IEEE Symposium on Mass Storage Systems and Technologies; Vol. 2022-January). IEEE Computer Society. https://doi.org/10.1109/MEMS51670.2022.9699570

Cartridge Device Capable of Spontaneous Solution Exchange for Sensor Cell Production. / Tanaka, Aoi; Takamori, Sho; Mimura, Hisatoshi et al.

35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022. IEEE Computer Society, 2022. p. 166-167 (IEEE Symposium on Mass Storage Systems and Technologies; Vol. 2022-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Tanaka, A, Takamori, S, Mimura, H, Osaki, T, Miki, N & Takeuchi, S 2022, Cartridge Device Capable of Spontaneous Solution Exchange for Sensor Cell Production. in 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022. IEEE Symposium on Mass Storage Systems and Technologies, vol. 2022-January, IEEE Computer Society, pp. 166-167, 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022, Tokyo, Japan, 22/1/9. https://doi.org/10.1109/MEMS51670.2022.9699570

Tanaka A, Takamori S, Mimura H, Osaki T, Miki N, Takeuchi S. Cartridge Device Capable of Spontaneous Solution Exchange for Sensor Cell Production. In 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022. IEEE Computer Society. 2022. p. 166-167. (IEEE Symposium on Mass Storage Systems and Technologies). doi: 10.1109/MEMS51670.2022.9699570

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abstract = "This paper reports a cartridge-type device to produce sensor cells with spontaneous solution exchange. Our group has developed a cell-based odorant sensor expressed olfactory receptors. These sensor cells presented excellent sensitivity and specificity, which would be useful for a wide range of sensor applications. However, production of such sensor cells requires multiple times of aseptic solution exchanges. In this work, we develop a cartridge-type cell culture device that utilizes capillary force for spontaneous solution exchange without pumps or tubes. When the cartridge is set to the exchange stage, the specified volume of solution is exchanged immediately. We experimentally examine the solution exchange, and demonstrate the response of a sensor cell to a chemical stimulus in the exchanged solution. We consider that the device would be useful to produce multiple types of sensor cells. ",

keywords = "Biohybrid odorant sensor, Capillary force, Insect olfactory receptor, Sensor cell, Solution exchange",

author = "Aoi Tanaka and Sho Takamori and Hisatoshi Mimura and Toshihisa Osaki and Norihisa Miki and Shoji Takeuchi",

note = "Funding Information: This work was partly supported by JSPS KAKENHI Grant Number JP21H05013, and by the Program for Building Regional Innovation Ecosystem of MEXT, Japan. Publisher Copyright: {\textcopyright} 2022 IEEE.; 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 ; Conference date: 09-01-2022 Through 13-01-2022",

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doi = "10.1109/MEMS51670.2022.9699570",

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AU - Miki, Norihisa

AU - Takeuchi, Shoji

N1 - Funding Information: This work was partly supported by JSPS KAKENHI Grant Number JP21H05013, and by the Program for Building Regional Innovation Ecosystem of MEXT, Japan. Publisher Copyright: © 2022 IEEE.

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N2 - This paper reports a cartridge-type device to produce sensor cells with spontaneous solution exchange. Our group has developed a cell-based odorant sensor expressed olfactory receptors. These sensor cells presented excellent sensitivity and specificity, which would be useful for a wide range of sensor applications. However, production of such sensor cells requires multiple times of aseptic solution exchanges. In this work, we develop a cartridge-type cell culture device that utilizes capillary force for spontaneous solution exchange without pumps or tubes. When the cartridge is set to the exchange stage, the specified volume of solution is exchanged immediately. We experimentally examine the solution exchange, and demonstrate the response of a sensor cell to a chemical stimulus in the exchanged solution. We consider that the device would be useful to produce multiple types of sensor cells.

AB - This paper reports a cartridge-type device to produce sensor cells with spontaneous solution exchange. Our group has developed a cell-based odorant sensor expressed olfactory receptors. These sensor cells presented excellent sensitivity and specificity, which would be useful for a wide range of sensor applications. However, production of such sensor cells requires multiple times of aseptic solution exchanges. In this work, we develop a cartridge-type cell culture device that utilizes capillary force for spontaneous solution exchange without pumps or tubes. When the cartridge is set to the exchange stage, the specified volume of solution is exchanged immediately. We experimentally examine the solution exchange, and demonstrate the response of a sensor cell to a chemical stimulus in the exchanged solution. We consider that the device would be useful to produce multiple types of sensor cells.

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Cartridge Device Capable of Spontaneous Solution Exchange for Sensor Cell Production

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