Caspase-3 activation is not responsible for vinblastine-induced Bcl-2 phosphorylation and G2/M arrest in human small cell lung carcinoma Ms-1 cells

Etsu Tashiro, Siro Simizu, Minoru Takada, Kazuo Umezawa, Masaya Imoto

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19 Citations (Scopus)

Abstract

Vinblastine arrests cells in the G2/M phase of the cell cycle and subsequently induces cell death by apoptosis. We found that treatment of cells with vinblastine induced phosphorylation of Bcl-2, resulting in the dissociation of Bcl-2 and Bax. Moreover, vinblastine-induced apoptosis was suppressed by an inhibitor of caspase-3, Ac-DEVD-CHO; and a 17-kDa active fragment of caspase-3 was detected following vinblastine treatment, suggesting that caspase-3 is involved in vinblastine-induced apoptosis. However, Ac-DEVD-CHO affected neither vinblastine-induced Bcl-2 phosphorylation nor vinblastine-induced G2/M arrest. Vinblastine caused G2/M arrest prior to apoptosis, whereas vinblastine-induced apoptosis was not dependent on the duration of the G2/M phase. Thus, vinblastine-induced apoptosis might be mediated by the phosphorylation of Bcl-2, resulting in Bcl-2 inactivation, and by subsequent activation of caspase-3.

Original languageEnglish
Pages (from-to)940-946
Number of pages7
JournalJapanese Journal of Cancer Research
Volume89
Issue number9
DOIs
Publication statusPublished - 1998 Sep

Keywords

  • Bcl-2
  • Caspase-3
  • G2/M arrest
  • Vinblastine

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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