Abstract
Programmed cell death is a mechanism through which organisms get rid of unwanted cells and is thought to be an important process in organogenesis. Although large-scale cell death is observed in the developing kidney, the precise roles of cell death in kidney organogenesis remain to be elucidated. To address this question, we prevented cell death in metanephric explants by applying caspase inhibitors. Administration of caspase inhibitors (Z-D- CH2DCB and Ac-DEVD-CHO) effectively prevented the cell death that is normally observed in nondifferentiating mesenchymal cells. Both ureteric bud branching and nephrogenesis were prevented by caspase inhibition. Our results suggest that caspases are crucial in kidney organogenesis and cell death in the nondifferentiating mesenchyme.
| Original language | English |
|---|---|
| Pages (from-to) | 423-429 |
| Number of pages | 7 |
| Journal | Experimental Cell Research |
| Volume | 248 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1999 May 1 |
| Externally published | Yes |
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Keywords
- Apoptosis
- Caspases
- Development
- Kidney
- Organogenesis
ASJC Scopus subject areas
- Cell Biology
Cite this
Caspase activity is required for nephrogenesis in the developing mouse metanephros. / Araki, Takashi; Saruta, Takao; Okano, Hideyuki; Miura, Masayuki.
In: Experimental Cell Research, Vol. 248, No. 2, 01.05.1999, p. 423-429.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Caspase activity is required for nephrogenesis in the developing mouse metanephros
AU - Araki, Takashi
AU - Saruta, Takao
AU - Okano, Hideyuki
AU - Miura, Masayuki
PY - 1999/5/1
Y1 - 1999/5/1
N2 - Programmed cell death is a mechanism through which organisms get rid of unwanted cells and is thought to be an important process in organogenesis. Although large-scale cell death is observed in the developing kidney, the precise roles of cell death in kidney organogenesis remain to be elucidated. To address this question, we prevented cell death in metanephric explants by applying caspase inhibitors. Administration of caspase inhibitors (Z-D- CH2DCB and Ac-DEVD-CHO) effectively prevented the cell death that is normally observed in nondifferentiating mesenchymal cells. Both ureteric bud branching and nephrogenesis were prevented by caspase inhibition. Our results suggest that caspases are crucial in kidney organogenesis and cell death in the nondifferentiating mesenchyme.
AB - Programmed cell death is a mechanism through which organisms get rid of unwanted cells and is thought to be an important process in organogenesis. Although large-scale cell death is observed in the developing kidney, the precise roles of cell death in kidney organogenesis remain to be elucidated. To address this question, we prevented cell death in metanephric explants by applying caspase inhibitors. Administration of caspase inhibitors (Z-D- CH2DCB and Ac-DEVD-CHO) effectively prevented the cell death that is normally observed in nondifferentiating mesenchymal cells. Both ureteric bud branching and nephrogenesis were prevented by caspase inhibition. Our results suggest that caspases are crucial in kidney organogenesis and cell death in the nondifferentiating mesenchyme.
KW - Apoptosis
KW - Caspases
KW - Development
KW - Kidney
KW - Organogenesis
UR - http://www.scopus.com/inward/record.url?scp=0033134126&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033134126&partnerID=8YFLogxK
U2 - 10.1006/excr.1999.4424
DO - 10.1006/excr.1999.4424
M3 - Article
C2 - 10222134
AN - SCOPUS:0033134126
VL - 248
SP - 423
EP - 429
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -