Cation-driven optical properties of artificial luciferases

Sung Bae Kim, Simon Miller, Nobuhiro Suzuki, Toshiya Senda, Ryo Nishihara, Koji Suzuki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The present study demonstrates cation-driven optical properties of artificial luciferases (ALucs) from copepod luciferases, as an optical readout for bioanalysis. An assignment of the supersecondary structure code (SSC) of ALucs revealed that ALucs carry a helix-loop-helix structure, which appears at the same sites of the EF-hands of typical Ca2+-binding proteins. A mutagenesis study shows that the EF-hand-like structure is a pivotal site for enzymatic activity. The effects of 20 kinds of mono- and multivalent cations on ALuc activities were estimated with column-purified ALuc16. High pH values boost the ALuc activities with both the native coelenterazine and an analog called 6-pi-OH-CTZ. Multivalent cations, Ca(II), Mg(II), and Cr(VI), elevate and prolong the ALuc activities, whereas Co(II), Cu(II) and Pb(II) greatly hamper the ALuc activities. Ca(II) greatly prolongs the optical intensities, suggesting a contribution to the structural robustness of ALucs. The inhibitory effect of multivalent cations on the ALuc activities was utilized for creating dose-response curves. The intrinsic cation-driven selectivity and optical intensity of ALucs enable researchers to constitute de novo biosensors for multivalent cations.

Original languageEnglish
Pages (from-to)955-960
Number of pages6
JournalAnalytical Sciences
Volume31
Issue number10
Publication statusPublished - 2015

Fingerprint

Luciferases
Cations
Optical properties
Monovalent Cations
Mutagenesis
Biosensors
Carrier Proteins

Keywords

  • Artificial luciferase
  • Bioluminescence
  • Metal cation
  • Supersecondary structure code

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Kim, S. B., Miller, S., Suzuki, N., Senda, T., Nishihara, R., & Suzuki, K. (2015). Cation-driven optical properties of artificial luciferases. Analytical Sciences, 31(10), 955-960.

Cation-driven optical properties of artificial luciferases. / Kim, Sung Bae; Miller, Simon; Suzuki, Nobuhiro; Senda, Toshiya; Nishihara, Ryo; Suzuki, Koji.

In: Analytical Sciences, Vol. 31, No. 10, 2015, p. 955-960.

Research output: Contribution to journalArticle

Kim, SB, Miller, S, Suzuki, N, Senda, T, Nishihara, R & Suzuki, K 2015, 'Cation-driven optical properties of artificial luciferases', Analytical Sciences, vol. 31, no. 10, pp. 955-960.
Kim SB, Miller S, Suzuki N, Senda T, Nishihara R, Suzuki K. Cation-driven optical properties of artificial luciferases. Analytical Sciences. 2015;31(10):955-960.
Kim, Sung Bae ; Miller, Simon ; Suzuki, Nobuhiro ; Senda, Toshiya ; Nishihara, Ryo ; Suzuki, Koji. / Cation-driven optical properties of artificial luciferases. In: Analytical Sciences. 2015 ; Vol. 31, No. 10. pp. 955-960.
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