Cbln1 regulates rapid formation and maintenance of excitatory synapses in mature cerebellar purkinje cells in vitro and in vivo

Aya Ishida, Eriko Miura, Kyoichi Emi, Keiko Matsuda, Takatoshi Iijima, Tetsuro Kondo, Kazuhisa Kohda, Masahiko Watanabe, Michisuke Yuzaki

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Although many synapse-organizing molecules have been identified in vitro, their functions in mature neurons in vivo have been mostly unexplored. Cbln1, which belongs to the C1q/tumor necrosis factor superfamily, is the most recently identified protein involved in synapse formation in the mammalian CNS. In the cerebellum, Cbln1 is predominantly produced and secreted from granule cells; cbln1-null mice show ataxia and a severe reduction in the number of synapses between Purkinje cells and parallel fibers (PFs), the axon bundle of granule cells. Here, we show that application of recombinant Cbln1 specifically and reversibly induced PF synapse formation in dissociated cbln1-null Purkinje cells in culture. Cbln1 also rapidly induced electrophysiologically functional and ultrastructurally normal PF synapses in acutely prepared cbln1-null cerebellar slices. Furthermore, a single injection of recombinant Cbln1 rescued severe ataxia in adult cbln1-null mice in vivo by completely, but transiently, restoring PF synapses. Therefore, Cbln1 is a unique synapse organizer that is required not only for the normal development of PF-Purkinje cell synapses but also for their maintenance in the mature cerebellum both in vitro and in vivo. Furthermore, our results indicate that Cbln1 can also rapidly organize new synapses in adult cerebellum, implying its therapeutic potential for cerebellar ataxic disorders.

Original languageEnglish
Pages (from-to)5920-5930
Number of pages11
JournalJournal of Neuroscience
Volume28
Issue number23
DOIs
Publication statusPublished - 2008 Jun 4

Fingerprint

Purkinje Cells
Synapses
Maintenance
Cerebellum
Ataxia
Cerebellar Diseases
In Vitro Techniques
Null Lymphocytes
Axons
Cell Culture Techniques
Tumor Necrosis Factor-alpha
Neurons
Injections

Keywords

  • C1q
  • Cerebellin
  • Cerebellum
  • Mouse
  • Parallel fiber
  • Purkinje cell
  • Synaptogenesis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Cbln1 regulates rapid formation and maintenance of excitatory synapses in mature cerebellar purkinje cells in vitro and in vivo. / Ishida, Aya; Miura, Eriko; Emi, Kyoichi; Matsuda, Keiko; Iijima, Takatoshi; Kondo, Tetsuro; Kohda, Kazuhisa; Watanabe, Masahiko; Yuzaki, Michisuke.

In: Journal of Neuroscience, Vol. 28, No. 23, 04.06.2008, p. 5920-5930.

Research output: Contribution to journalArticle

Ishida, Aya ; Miura, Eriko ; Emi, Kyoichi ; Matsuda, Keiko ; Iijima, Takatoshi ; Kondo, Tetsuro ; Kohda, Kazuhisa ; Watanabe, Masahiko ; Yuzaki, Michisuke. / Cbln1 regulates rapid formation and maintenance of excitatory synapses in mature cerebellar purkinje cells in vitro and in vivo. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 23. pp. 5920-5930.
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