CC-Chemokine receptor CCR7: A key molecule for lymph node metastasis in esophageal squamous cell carcinoma

Tomoyuki Irino, Hiroya Takeuchi, Sachiko Matsuda, Yoshiro Saikawa, Hirofumi Kawakubo, Norihito Wada, Tsunehiro Takahashi, Rieko Nakamura, Kazumasa Fukuda, Tai Omori, Yuukou Kitagawa

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: CC-chemokine receptor 7 (CCR7), a known lymph node homing receptor for immune cells, has been reported as a key molecule in lymph node metastasis. We hypothesized a clinicopathological correlation and functional causality between CCR7 expression and lymph node metastasis in patients with esophageal squamous cell carcinoma (ESCC).Methods: We performed immunohistochemical analysis of 105 consecutive and 61 exclusive pathological T1 ESCC patients, followed by adhesion assay and in vivo experiment using a newly developed lymph node metastasis mouse model. The adhesive ability in response to CC-chemokine ligand 21/secondary lymphoid-tissue chemokine (CCL21/SLC) was assessed in the presence or absence of lymphatic endothelial cells and anti-CCR7 antibody. We established a heterotopic transplantation mouse model and analyzed lymph node metastasis by quantitative real-time RT-PCR.Results: Positive CCR7 expression in immunohistochemistory was detected in 28 (27%) of 105 consecutive patients and 17 (28%) of 61 T1 patients, which significantly correlated with lymph node metastasis (p = 0.037 and p = 0.040, respectively) and poor five-year survival (p = 0.013 and p = 0.012, respectively). Adhesion assay revealed an enhanced adhesive ability of CCR7-expressing cells in response to CCL21/SLC, in particular, in the presence of lymphatic endothelial cells (p = 0.005). In the mouse model, lymph nodes from mice transplanted with CCR7-expressing cells showed significantly higher DNA levels at 5 weeks (p = 0.019), indicating a high metastatic potential of CCR7-expressing cells.Conclusion: These results demonstrated the significant clinicopathological relationship and functional causality between CCR7 expression and lymph node metastasis in ESCC patients.

Original languageEnglish
Article number291
JournalBMC Cancer
Volume14
Issue number1
DOIs
Publication statusPublished - 2014 Apr 26

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CCR7 Receptors
CCR Receptors
Lymph Nodes
Neoplasm Metastasis
Chemokine CCL21
CC Chemokines
Causality
Adhesives
Lymphocyte Homing Receptors
Endothelial Cells
Heterotopic Transplantation
Ligands
Esophageal Squamous Cell Carcinoma
Real-Time Polymerase Chain Reaction

Keywords

  • CCL21
  • CCR7
  • Chemokine
  • Chemokine receptor
  • Esophageal squamous cell carcinoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Genetics
  • Medicine(all)

Cite this

CC-Chemokine receptor CCR7 : A key molecule for lymph node metastasis in esophageal squamous cell carcinoma. / Irino, Tomoyuki; Takeuchi, Hiroya; Matsuda, Sachiko; Saikawa, Yoshiro; Kawakubo, Hirofumi; Wada, Norihito; Takahashi, Tsunehiro; Nakamura, Rieko; Fukuda, Kazumasa; Omori, Tai; Kitagawa, Yuukou.

In: BMC Cancer, Vol. 14, No. 1, 291, 26.04.2014.

Research output: Contribution to journalArticle

Irino, Tomoyuki ; Takeuchi, Hiroya ; Matsuda, Sachiko ; Saikawa, Yoshiro ; Kawakubo, Hirofumi ; Wada, Norihito ; Takahashi, Tsunehiro ; Nakamura, Rieko ; Fukuda, Kazumasa ; Omori, Tai ; Kitagawa, Yuukou. / CC-Chemokine receptor CCR7 : A key molecule for lymph node metastasis in esophageal squamous cell carcinoma. In: BMC Cancer. 2014 ; Vol. 14, No. 1.
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abstract = "Background: CC-chemokine receptor 7 (CCR7), a known lymph node homing receptor for immune cells, has been reported as a key molecule in lymph node metastasis. We hypothesized a clinicopathological correlation and functional causality between CCR7 expression and lymph node metastasis in patients with esophageal squamous cell carcinoma (ESCC).Methods: We performed immunohistochemical analysis of 105 consecutive and 61 exclusive pathological T1 ESCC patients, followed by adhesion assay and in vivo experiment using a newly developed lymph node metastasis mouse model. The adhesive ability in response to CC-chemokine ligand 21/secondary lymphoid-tissue chemokine (CCL21/SLC) was assessed in the presence or absence of lymphatic endothelial cells and anti-CCR7 antibody. We established a heterotopic transplantation mouse model and analyzed lymph node metastasis by quantitative real-time RT-PCR.Results: Positive CCR7 expression in immunohistochemistory was detected in 28 (27{\%}) of 105 consecutive patients and 17 (28{\%}) of 61 T1 patients, which significantly correlated with lymph node metastasis (p = 0.037 and p = 0.040, respectively) and poor five-year survival (p = 0.013 and p = 0.012, respectively). Adhesion assay revealed an enhanced adhesive ability of CCR7-expressing cells in response to CCL21/SLC, in particular, in the presence of lymphatic endothelial cells (p = 0.005). In the mouse model, lymph nodes from mice transplanted with CCR7-expressing cells showed significantly higher DNA levels at 5 weeks (p = 0.019), indicating a high metastatic potential of CCR7-expressing cells.Conclusion: These results demonstrated the significant clinicopathological relationship and functional causality between CCR7 expression and lymph node metastasis in ESCC patients.",
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T1 - CC-Chemokine receptor CCR7

T2 - A key molecule for lymph node metastasis in esophageal squamous cell carcinoma

AU - Irino, Tomoyuki

AU - Takeuchi, Hiroya

AU - Matsuda, Sachiko

AU - Saikawa, Yoshiro

AU - Kawakubo, Hirofumi

AU - Wada, Norihito

AU - Takahashi, Tsunehiro

AU - Nakamura, Rieko

AU - Fukuda, Kazumasa

AU - Omori, Tai

AU - Kitagawa, Yuukou

PY - 2014/4/26

Y1 - 2014/4/26

N2 - Background: CC-chemokine receptor 7 (CCR7), a known lymph node homing receptor for immune cells, has been reported as a key molecule in lymph node metastasis. We hypothesized a clinicopathological correlation and functional causality between CCR7 expression and lymph node metastasis in patients with esophageal squamous cell carcinoma (ESCC).Methods: We performed immunohistochemical analysis of 105 consecutive and 61 exclusive pathological T1 ESCC patients, followed by adhesion assay and in vivo experiment using a newly developed lymph node metastasis mouse model. The adhesive ability in response to CC-chemokine ligand 21/secondary lymphoid-tissue chemokine (CCL21/SLC) was assessed in the presence or absence of lymphatic endothelial cells and anti-CCR7 antibody. We established a heterotopic transplantation mouse model and analyzed lymph node metastasis by quantitative real-time RT-PCR.Results: Positive CCR7 expression in immunohistochemistory was detected in 28 (27%) of 105 consecutive patients and 17 (28%) of 61 T1 patients, which significantly correlated with lymph node metastasis (p = 0.037 and p = 0.040, respectively) and poor five-year survival (p = 0.013 and p = 0.012, respectively). Adhesion assay revealed an enhanced adhesive ability of CCR7-expressing cells in response to CCL21/SLC, in particular, in the presence of lymphatic endothelial cells (p = 0.005). In the mouse model, lymph nodes from mice transplanted with CCR7-expressing cells showed significantly higher DNA levels at 5 weeks (p = 0.019), indicating a high metastatic potential of CCR7-expressing cells.Conclusion: These results demonstrated the significant clinicopathological relationship and functional causality between CCR7 expression and lymph node metastasis in ESCC patients.

AB - Background: CC-chemokine receptor 7 (CCR7), a known lymph node homing receptor for immune cells, has been reported as a key molecule in lymph node metastasis. We hypothesized a clinicopathological correlation and functional causality between CCR7 expression and lymph node metastasis in patients with esophageal squamous cell carcinoma (ESCC).Methods: We performed immunohistochemical analysis of 105 consecutive and 61 exclusive pathological T1 ESCC patients, followed by adhesion assay and in vivo experiment using a newly developed lymph node metastasis mouse model. The adhesive ability in response to CC-chemokine ligand 21/secondary lymphoid-tissue chemokine (CCL21/SLC) was assessed in the presence or absence of lymphatic endothelial cells and anti-CCR7 antibody. We established a heterotopic transplantation mouse model and analyzed lymph node metastasis by quantitative real-time RT-PCR.Results: Positive CCR7 expression in immunohistochemistory was detected in 28 (27%) of 105 consecutive patients and 17 (28%) of 61 T1 patients, which significantly correlated with lymph node metastasis (p = 0.037 and p = 0.040, respectively) and poor five-year survival (p = 0.013 and p = 0.012, respectively). Adhesion assay revealed an enhanced adhesive ability of CCR7-expressing cells in response to CCL21/SLC, in particular, in the presence of lymphatic endothelial cells (p = 0.005). In the mouse model, lymph nodes from mice transplanted with CCR7-expressing cells showed significantly higher DNA levels at 5 weeks (p = 0.019), indicating a high metastatic potential of CCR7-expressing cells.Conclusion: These results demonstrated the significant clinicopathological relationship and functional causality between CCR7 expression and lymph node metastasis in ESCC patients.

KW - CCL21

KW - CCR7

KW - Chemokine

KW - Chemokine receptor

KW - Esophageal squamous cell carcinoma

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DO - 10.1186/1471-2407-14-291

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