CCA [N-(2-carboxyphenyl)-4-Chloroanthranilic acid disodium salt], a newly developed immunomodulating drug, suppresses T-cell activation by acting on macrophages

Tsutomu Takeuchi, Jun Koide, Osamu Hosono, Makoto Takano, Tohru Abe

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The cellular mechanism of action of a newly developed drug, CCA, N-(2-carboxyphenyl)-4-chloroanthranilic acid disodium salt, on PHA-, autologous mixed lymphocyte reaction (AMLR)-, and phorbol myristate acetate (PMA)-stimulated T-cell proliferation was investigated. Addition of 50 μg of CCA per milliliter suppressed PHA- and AMLR-stimulaled T-cell proliferation. In contrast. CCA failed to suppress PMA-stimulated macrophage-depleted T-cell proliferation. After treatment of T cells or macrophages with CCA for 12 h, recombined T cells and macrophages were stimulated with phytohemagglutinin. [3H]Thymidine incorporation by T cells was suppressed when macrophages but not T cells were treated with CCA. These results indicate that CCA suppresses T-cell proliferation by acting on macrophages. The mechanism involved in this suppression of CCA was due to the loss of Ia antigen on macrophages and the loss of interleukin-1 (IL-1) secretion from macrophages.

Original languageEnglish
Pages (from-to)125-135
Number of pages11
JournalInflammation
Volume13
Issue number2
DOIs
Publication statusPublished - 1989 Apr
Externally publishedYes

Fingerprint

Salts
Macrophages
T-Lymphocytes
Pharmaceutical Preparations
Cell Proliferation
Mixed Lymphocyte Culture Test
Tetradecanoylphorbol Acetate
lobenzarit
Histocompatibility Antigens Class II
Phytohemagglutinins
Interleukin-1
Thymidine

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology
  • Cell Biology

Cite this

CCA [N-(2-carboxyphenyl)-4-Chloroanthranilic acid disodium salt], a newly developed immunomodulating drug, suppresses T-cell activation by acting on macrophages. / Takeuchi, Tsutomu; Koide, Jun; Hosono, Osamu; Takano, Makoto; Abe, Tohru.

In: Inflammation, Vol. 13, No. 2, 04.1989, p. 125-135.

Research output: Contribution to journalArticle

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