Abstract
Multiple sclerosis (MS) is a demyelinating disease of the CNS that is presumably mediated by CD4+ autoimmune T cells. Although both Th1 and Th17 cells have the potential to cause inflammatory CNS pathology in rodents, the identity of pathogenic T cells remains unclear in human MS. Given that each Th cell subset preferentially expresses specific chemokine receptors, we were interested to know whether T cells defined by a particular chemokine receptor profile play an active role in the pathogenesis of MS. In this article, we report that CCR2+CCR5+ T cells constitute a unique population selectively enriched in the cerebrospinal fluid of MS patients during relapse but not in patients with other neurologic diseases. After polyclonal stimulation, the CCR2+ CCR5+ T cells exhibited a distinct ability to produce matrix metalloproteinase-9 and osteopontin, which are involved in the CNS pathology of MS. Furthermore, after TCR stimulation, the CCR2+CCR5+ T cells showed a higher invasive potential across an in vitro blood-brain barrier model compared with other T cells. Of note, the CCR2+CCR5+ T cells from MS patients in relapse are reactive to myelin basic protein, as assessed by production of IFN-γ. We also demonstrated that the CCR6-, but not the CCR6+, population within CCR2+CCR5+ T cells was highly enriched in the cerebrospinal fluid during MS relapse (p < 0.0005) and expressed higher levels of IFN-γ and matrix metalloproteinase-9. Taken together, we propose that autoimmune CCR2+CCR5+ CCR6- Th1 cells play a crucial role in the pathogenesis of MS.
| Original language | English |
|---|---|
| Pages (from-to) | 5057-5065 |
| Number of pages | 9 |
| Journal | Journal of Immunology |
| Volume | 189 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2012 Nov 15 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
CCR2+CCR5+ T cells produce matrix metalloproteinase-9 and osteopontin in the pathogenesis of multiple sclerosis. / Sato, Wakiro; Tomita, Atsuko; Ichikawa, Daijyu; Lin, Youwei; Kishida, Hitaru; Miyake, Sachiko; Ogawa, Masafumi; Okamoto, Tomoko; Murata, Miho; Kuroiwa, Yoshiyuki; Aranami, Toshimasa; Yamamura, Takashi.
In: Journal of Immunology, Vol. 189, No. 10, 15.11.2012, p. 5057-5065.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CCR2+CCR5+ T cells produce matrix metalloproteinase-9 and osteopontin in the pathogenesis of multiple sclerosis
AU - Sato, Wakiro
AU - Tomita, Atsuko
AU - Ichikawa, Daijyu
AU - Lin, Youwei
AU - Kishida, Hitaru
AU - Miyake, Sachiko
AU - Ogawa, Masafumi
AU - Okamoto, Tomoko
AU - Murata, Miho
AU - Kuroiwa, Yoshiyuki
AU - Aranami, Toshimasa
AU - Yamamura, Takashi
PY - 2012/11/15
Y1 - 2012/11/15
N2 - Multiple sclerosis (MS) is a demyelinating disease of the CNS that is presumably mediated by CD4+ autoimmune T cells. Although both Th1 and Th17 cells have the potential to cause inflammatory CNS pathology in rodents, the identity of pathogenic T cells remains unclear in human MS. Given that each Th cell subset preferentially expresses specific chemokine receptors, we were interested to know whether T cells defined by a particular chemokine receptor profile play an active role in the pathogenesis of MS. In this article, we report that CCR2+CCR5+ T cells constitute a unique population selectively enriched in the cerebrospinal fluid of MS patients during relapse but not in patients with other neurologic diseases. After polyclonal stimulation, the CCR2+ CCR5+ T cells exhibited a distinct ability to produce matrix metalloproteinase-9 and osteopontin, which are involved in the CNS pathology of MS. Furthermore, after TCR stimulation, the CCR2+CCR5+ T cells showed a higher invasive potential across an in vitro blood-brain barrier model compared with other T cells. Of note, the CCR2+CCR5+ T cells from MS patients in relapse are reactive to myelin basic protein, as assessed by production of IFN-γ. We also demonstrated that the CCR6-, but not the CCR6+, population within CCR2+CCR5+ T cells was highly enriched in the cerebrospinal fluid during MS relapse (p < 0.0005) and expressed higher levels of IFN-γ and matrix metalloproteinase-9. Taken together, we propose that autoimmune CCR2+CCR5+ CCR6- Th1 cells play a crucial role in the pathogenesis of MS.
AB - Multiple sclerosis (MS) is a demyelinating disease of the CNS that is presumably mediated by CD4+ autoimmune T cells. Although both Th1 and Th17 cells have the potential to cause inflammatory CNS pathology in rodents, the identity of pathogenic T cells remains unclear in human MS. Given that each Th cell subset preferentially expresses specific chemokine receptors, we were interested to know whether T cells defined by a particular chemokine receptor profile play an active role in the pathogenesis of MS. In this article, we report that CCR2+CCR5+ T cells constitute a unique population selectively enriched in the cerebrospinal fluid of MS patients during relapse but not in patients with other neurologic diseases. After polyclonal stimulation, the CCR2+ CCR5+ T cells exhibited a distinct ability to produce matrix metalloproteinase-9 and osteopontin, which are involved in the CNS pathology of MS. Furthermore, after TCR stimulation, the CCR2+CCR5+ T cells showed a higher invasive potential across an in vitro blood-brain barrier model compared with other T cells. Of note, the CCR2+CCR5+ T cells from MS patients in relapse are reactive to myelin basic protein, as assessed by production of IFN-γ. We also demonstrated that the CCR6-, but not the CCR6+, population within CCR2+CCR5+ T cells was highly enriched in the cerebrospinal fluid during MS relapse (p < 0.0005) and expressed higher levels of IFN-γ and matrix metalloproteinase-9. Taken together, we propose that autoimmune CCR2+CCR5+ CCR6- Th1 cells play a crucial role in the pathogenesis of MS.
UR - http://www.scopus.com/inward/record.url?scp=84868513673&partnerID=8YFLogxK
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U2 - 10.4049/jimmunol.1202026
DO - 10.4049/jimmunol.1202026
M3 - Article
C2 - 23071279
AN - SCOPUS:84868513673
VL - 189
SP - 5057
EP - 5065
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 10
ER -