CCR6hiCD11cint B cells promote M-cell differentiation in Peyer's patch

Masashi Ebisawa, Kouji Hase, Daisuke Takahashi, Hiroshi Kitamura, Kathryn A. Knoop, Ifor R. Williams, Hiroshi Ohno

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

M cells are responsible for uptake of mucosal antigens in Peyer's patches (PPs). Differentiation of M cells is thought to be induced by interactions between follicle-associated epithelium and PP cells; however, it remains elusive what types of immune cells function as M-cell inducers. Here, we attempted to identify the cells that serve as an M-cell inducer in PP. We found that a unique B-cell subset characterized by CCR6hiCD11cint resided in the subepithelial dome (SED) in mouse PP. CCR6hiCD11cint B cells showed chemotactic migration in response to CCL20. Furthermore, this unique B-cell subset substantially decreased in PP of CCR6-deficient mice, indicating that the SED localization of CCR6hiCD11cint B cells is most likely regulated by the CCL20-CCR6 system. Concomitantly, CCR6 deficiency caused remarkable decrement of M cells. Moreover, adoptive transfer of CCR6hiCD11cint B cells from wild-type mice restored the M-cell decrement in CCR6-deficient mice. Collectively, the spatial regulation of CCR6hiCD11cint B cells via the CCL20-CCR6 system may play a vital role in M-cell differentiation in mice.

Original languageEnglish
Pages (from-to)261-269
Number of pages9
JournalInternational Immunology
Volume23
Issue number4
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Peyer's Patches
Cell Differentiation
B-Lymphocytes
B-Lymphocyte Subsets
Helper-Inducer T-Lymphocytes
Adoptive Transfer
Epithelium
Antigens

Keywords

  • CCR6
  • CD11c B cells
  • Cell differentiation
  • M cells
  • Peyer's patch

ASJC Scopus subject areas

  • Immunology

Cite this

CCR6hiCD11cint B cells promote M-cell differentiation in Peyer's patch. / Ebisawa, Masashi; Hase, Kouji; Takahashi, Daisuke; Kitamura, Hiroshi; Knoop, Kathryn A.; Williams, Ifor R.; Ohno, Hiroshi.

In: International Immunology, Vol. 23, No. 4, 2011, p. 261-269.

Research output: Contribution to journalArticle

Ebisawa, Masashi ; Hase, Kouji ; Takahashi, Daisuke ; Kitamura, Hiroshi ; Knoop, Kathryn A. ; Williams, Ifor R. ; Ohno, Hiroshi. / CCR6hiCD11cint B cells promote M-cell differentiation in Peyer's patch. In: International Immunology. 2011 ; Vol. 23, No. 4. pp. 261-269.
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