CCR9+ plasmacytoid dendritic cells in the small intestine suppress development of intestinal inflammation in mice

Shinta Mizuno, Takanori Kanai, Yohei Mikami, Tomohisa Sujino, Yuichi Ono, Atsushi Hayashi, Tango Handa, Atsuhiro Matsumoto, Nobuhiro Nakamoto, Katsuyoshi Matsuoka, Tadakazu Hisamatsu, Hiromasa Takaishi, Toshifumi Hibi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Almost all mice lacking specific molecules associated with regulatory T cells or barrier function develop intestinal inflammation in the colon, but not in the small intestine (SI). Therefore, intestinal homeostasis of the SI may be tightly controlled by other mechanisms. To determine the role of CCR9+ plasmacytoid dendritic cells (pDCs) in intestinal homeostasis of the SI we transferred CD4+CD45RBhigh T cells into ccr9-/-×rag-2-/- mice. We showed that CCL25, a counterpart chemokine for CCR9, is constitutively expressed in the SI but not the colon and spleen of rag-2-/- or ccr9-/-×rag-2-/- mice before or after transfer of CD4+CD45RBhigh T cells. The ccr9-/-×rag-2-/- mice did not develop spontaneous intestinal inflammation in the SI and colon. Mice of both genotype where CD4+CD45RBhigh T cells were transferred developed colitis. However, the ccr9-/-×rag-2-/- mice also developed ileitis with marked infiltration of Th1 cells. These results suggest that CCR9+ pDCs are possibly small, regulatory, antigen-presenting cells of the intestine that suppress intestinal inflammation.

Original languageEnglish
Pages (from-to)64-69
Number of pages6
JournalImmunology Letters
Volume146
Issue number1-2
DOIs
Publication statusPublished - 2012 Aug 30

Fingerprint

Dendritic Cells
Small Intestine
Inflammation
Colon
T-Lymphocytes
Homeostasis
Ileitis
Th1 Cells
Antigen-Presenting Cells
Regulatory T-Lymphocytes
Colitis
Chemokines
Intestines
Spleen
Genotype

Keywords

  • CCR9/CCL25
  • Colitis
  • Ileitis
  • Immune regulation
  • Plasmacytoid dendritic cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

CCR9+ plasmacytoid dendritic cells in the small intestine suppress development of intestinal inflammation in mice. / Mizuno, Shinta; Kanai, Takanori; Mikami, Yohei; Sujino, Tomohisa; Ono, Yuichi; Hayashi, Atsushi; Handa, Tango; Matsumoto, Atsuhiro; Nakamoto, Nobuhiro; Matsuoka, Katsuyoshi; Hisamatsu, Tadakazu; Takaishi, Hiromasa; Hibi, Toshifumi.

In: Immunology Letters, Vol. 146, No. 1-2, 30.08.2012, p. 64-69.

Research output: Contribution to journalArticle

Mizuno, S, Kanai, T, Mikami, Y, Sujino, T, Ono, Y, Hayashi, A, Handa, T, Matsumoto, A, Nakamoto, N, Matsuoka, K, Hisamatsu, T, Takaishi, H & Hibi, T 2012, 'CCR9+ plasmacytoid dendritic cells in the small intestine suppress development of intestinal inflammation in mice', Immunology Letters, vol. 146, no. 1-2, pp. 64-69. https://doi.org/10.1016/j.imlet.2012.05.001
Mizuno, Shinta ; Kanai, Takanori ; Mikami, Yohei ; Sujino, Tomohisa ; Ono, Yuichi ; Hayashi, Atsushi ; Handa, Tango ; Matsumoto, Atsuhiro ; Nakamoto, Nobuhiro ; Matsuoka, Katsuyoshi ; Hisamatsu, Tadakazu ; Takaishi, Hiromasa ; Hibi, Toshifumi. / CCR9+ plasmacytoid dendritic cells in the small intestine suppress development of intestinal inflammation in mice. In: Immunology Letters. 2012 ; Vol. 146, No. 1-2. pp. 64-69.
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