CD133 negatively regulates tumorigenicity via AKT pathway in synovial sarcoma

Taichi Kimura; Lei Wang; Kouichi Tabu; Hiroshi Nishihara; Yuji Mashita; Naoyuki Kikuchi; Mishie Tanino; Hiroaki Hiraga; Shinya Tanaka

doi:10.3109/07357907.2012.672607

CD133 negatively regulates tumorigenicity via AKT pathway in synovial sarcoma

Taichi Kimura, Lei Wang, Kouichi Tabu, Hiroshi Nishihara, Yuji Mashita, Naoyuki Kikuchi, Mishie Tanino, Hiroaki Hiraga, Shinya Tanaka

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Synovial sarcoma (SS) is an aggressive tumor that accounts for almost 10 of all soft tissue sarcomas. In this study, we found the expression of CD133 in human SS specimens, thus, we focused on the function of CD133 in SS. Separation of the CD133-positive and -negative subpopulations in SS cell lines clarified that the CD133-negative subpopulation exhibited enhanced growth and hyperphosphorylation of AKT. Treatment of Akt inhibitor suppressed the cell growth of CD133-negative subpopulation to the levels of CD133-positive cells. These results suggest that CD133 has negative effect on the growth of cells through AKT-dependent signalling pathway.

Original language	English
Pages (from-to)	390-397
Number of pages	8
Journal	Cancer Investigation
Volume	30
Issue number	5
DOIs	https://doi.org/10.3109/07357907.2012.672607
Publication status	Published - 2012 Jun
Externally published	Yes

Keywords

CD133
Cancer stem cell
Synovial sarcoma

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3109/07357907.2012.672607

Cite this

@article{5dceac9374b84755a5da6b85f13d9a18,

title = "CD133 negatively regulates tumorigenicity via AKT pathway in synovial sarcoma",

abstract = "Synovial sarcoma (SS) is an aggressive tumor that accounts for almost 10 of all soft tissue sarcomas. In this study, we found the expression of CD133 in human SS specimens, thus, we focused on the function of CD133 in SS. Separation of the CD133-positive and -negative subpopulations in SS cell lines clarified that the CD133-negative subpopulation exhibited enhanced growth and hyperphosphorylation of AKT. Treatment of Akt inhibitor suppressed the cell growth of CD133-negative subpopulation to the levels of CD133-positive cells. These results suggest that CD133 has negative effect on the growth of cells through AKT-dependent signalling pathway.",

keywords = "CD133, Cancer stem cell, Synovial sarcoma",

author = "Taichi Kimura and Lei Wang and Kouichi Tabu and Hiroshi Nishihara and Yuji Mashita and Naoyuki Kikuchi and Mishie Tanino and Hiroaki Hiraga and Shinya Tanaka",

note = "Funding Information: We thank Miho Nodagashira and Eiko Aoyanagi for the excellent technical assistance of immunohistochemistry. This work was supported in part by Grant-in-Aid for Scientific Research on Priority Area “Cancer”(20013001) from the Ministry of Education, Culture, Sports, Science and Technology and for Scientific Research (B) (19390386) from the Japan Society for the Promotion of Science.",

year = "2012",

month = jun,

doi = "10.3109/07357907.2012.672607",

language = "English",

volume = "30",

pages = "390--397",

journal = "Cancer Investigation",

issn = "0735-7907",

publisher = "Informa Healthcare",

number = "5",

}

TY - JOUR

T1 - CD133 negatively regulates tumorigenicity via AKT pathway in synovial sarcoma

AU - Kimura, Taichi

AU - Wang, Lei

AU - Tabu, Kouichi

AU - Nishihara, Hiroshi

AU - Mashita, Yuji

AU - Kikuchi, Naoyuki

AU - Tanino, Mishie

AU - Hiraga, Hiroaki

AU - Tanaka, Shinya

N1 - Funding Information: We thank Miho Nodagashira and Eiko Aoyanagi for the excellent technical assistance of immunohistochemistry. This work was supported in part by Grant-in-Aid for Scientific Research on Priority Area “Cancer”(20013001) from the Ministry of Education, Culture, Sports, Science and Technology and for Scientific Research (B) (19390386) from the Japan Society for the Promotion of Science.

PY - 2012/6

Y1 - 2012/6

N2 - Synovial sarcoma (SS) is an aggressive tumor that accounts for almost 10 of all soft tissue sarcomas. In this study, we found the expression of CD133 in human SS specimens, thus, we focused on the function of CD133 in SS. Separation of the CD133-positive and -negative subpopulations in SS cell lines clarified that the CD133-negative subpopulation exhibited enhanced growth and hyperphosphorylation of AKT. Treatment of Akt inhibitor suppressed the cell growth of CD133-negative subpopulation to the levels of CD133-positive cells. These results suggest that CD133 has negative effect on the growth of cells through AKT-dependent signalling pathway.

AB - Synovial sarcoma (SS) is an aggressive tumor that accounts for almost 10 of all soft tissue sarcomas. In this study, we found the expression of CD133 in human SS specimens, thus, we focused on the function of CD133 in SS. Separation of the CD133-positive and -negative subpopulations in SS cell lines clarified that the CD133-negative subpopulation exhibited enhanced growth and hyperphosphorylation of AKT. Treatment of Akt inhibitor suppressed the cell growth of CD133-negative subpopulation to the levels of CD133-positive cells. These results suggest that CD133 has negative effect on the growth of cells through AKT-dependent signalling pathway.

KW - CD133

KW - Cancer stem cell

KW - Synovial sarcoma

UR - http://www.scopus.com/inward/record.url?scp=84861079822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861079822&partnerID=8YFLogxK

U2 - 10.3109/07357907.2012.672607

DO - 10.3109/07357907.2012.672607

M3 - Article

C2 - 22571340

AN - SCOPUS:84861079822

SN - 0735-7907

VL - 30

SP - 390

EP - 397

JO - Cancer Investigation

JF - Cancer Investigation

IS - 5

ER -

CD133 negatively regulates tumorigenicity via AKT pathway in synovial sarcoma

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this