CD24 is expressed specifically in the nucleus pulposus of intervertebral discs

Nobuyuki Fujita, Takeshi Miyamoto, Jun Ichi Imai, Naobumi Hosogane, Toru Suzuki, Mitsuru Yagi, Kozo Morita, Ken Ninomiya, Kana Miyamoto, Hironari Takaishi, Morio Matsumoto, Hideo Morioka, Hiroo Yabe, Kazuhiro Chiba, Shinya Watanabe, Yoshiaki Toyama, Toshio Suda

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Intervertebral disc (IVD) consists of a soft gelatinous material in its center, the nucleus pulposus (NP), bounded peripherally by fibrocartilage, annulus fibrosus (AF). Despite the number of patients with IVD degeneration, gene expression analysis has not been undertaken in NP and therefore little is known about the molecular markers expressed in NP. Here, we undertook a microarray screen in NP with the other nine tissues to identify the specific cell surface markers for NP. Five membrane associating molecules out of 10,490 genes were identified as highly expressing genes in NP compared with the other tissues. Among them, we identified CD24, a glycosylphosphatidylinositol (GPI) anchor protein as a cell surface marker for NP. CD24 expression was also detected in the herniated NP and chordoma, a malignant primary tumor derived from notochordal cells, while it was absent in chondrosarcoma. Therefore, CD24 is a molecular marker for NP as well as the diseases of IVD.

Original languageEnglish
Pages (from-to)1890-1896
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume338
Issue number4
DOIs
Publication statusPublished - 2005 Dec 30

Fingerprint

Intervertebral Disc
Genes
Tissue
Glycosylphosphatidylinositols
Microarrays
Gene expression
Tumors
Membranes
Molecules
Proteins
Fibrocartilage
Chordoma
Nucleus Pulposus
Intervertebral Disc Degeneration
Chondrosarcoma
Gene Expression

Keywords

  • CD24
  • Intervertebral disc
  • Nucleus pulposus

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

CD24 is expressed specifically in the nucleus pulposus of intervertebral discs. / Fujita, Nobuyuki; Miyamoto, Takeshi; Imai, Jun Ichi; Hosogane, Naobumi; Suzuki, Toru; Yagi, Mitsuru; Morita, Kozo; Ninomiya, Ken; Miyamoto, Kana; Takaishi, Hironari; Matsumoto, Morio; Morioka, Hideo; Yabe, Hiroo; Chiba, Kazuhiro; Watanabe, Shinya; Toyama, Yoshiaki; Suda, Toshio.

In: Biochemical and Biophysical Research Communications, Vol. 338, No. 4, 30.12.2005, p. 1890-1896.

Research output: Contribution to journalArticle

Fujita, N, Miyamoto, T, Imai, JI, Hosogane, N, Suzuki, T, Yagi, M, Morita, K, Ninomiya, K, Miyamoto, K, Takaishi, H, Matsumoto, M, Morioka, H, Yabe, H, Chiba, K, Watanabe, S, Toyama, Y & Suda, T 2005, 'CD24 is expressed specifically in the nucleus pulposus of intervertebral discs', Biochemical and Biophysical Research Communications, vol. 338, no. 4, pp. 1890-1896. https://doi.org/10.1016/j.bbrc.2005.10.166
Fujita, Nobuyuki ; Miyamoto, Takeshi ; Imai, Jun Ichi ; Hosogane, Naobumi ; Suzuki, Toru ; Yagi, Mitsuru ; Morita, Kozo ; Ninomiya, Ken ; Miyamoto, Kana ; Takaishi, Hironari ; Matsumoto, Morio ; Morioka, Hideo ; Yabe, Hiroo ; Chiba, Kazuhiro ; Watanabe, Shinya ; Toyama, Yoshiaki ; Suda, Toshio. / CD24 is expressed specifically in the nucleus pulposus of intervertebral discs. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 338, No. 4. pp. 1890-1896.
@article{ccc1564311ce412f845ff61a73fa8042,
title = "CD24 is expressed specifically in the nucleus pulposus of intervertebral discs",
abstract = "Intervertebral disc (IVD) consists of a soft gelatinous material in its center, the nucleus pulposus (NP), bounded peripherally by fibrocartilage, annulus fibrosus (AF). Despite the number of patients with IVD degeneration, gene expression analysis has not been undertaken in NP and therefore little is known about the molecular markers expressed in NP. Here, we undertook a microarray screen in NP with the other nine tissues to identify the specific cell surface markers for NP. Five membrane associating molecules out of 10,490 genes were identified as highly expressing genes in NP compared with the other tissues. Among them, we identified CD24, a glycosylphosphatidylinositol (GPI) anchor protein as a cell surface marker for NP. CD24 expression was also detected in the herniated NP and chordoma, a malignant primary tumor derived from notochordal cells, while it was absent in chondrosarcoma. Therefore, CD24 is a molecular marker for NP as well as the diseases of IVD.",
keywords = "CD24, Intervertebral disc, Nucleus pulposus",
author = "Nobuyuki Fujita and Takeshi Miyamoto and Imai, {Jun Ichi} and Naobumi Hosogane and Toru Suzuki and Mitsuru Yagi and Kozo Morita and Ken Ninomiya and Kana Miyamoto and Hironari Takaishi and Morio Matsumoto and Hideo Morioka and Hiroo Yabe and Kazuhiro Chiba and Shinya Watanabe and Yoshiaki Toyama and Toshio Suda",
year = "2005",
month = "12",
day = "30",
doi = "10.1016/j.bbrc.2005.10.166",
language = "English",
volume = "338",
pages = "1890--1896",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - CD24 is expressed specifically in the nucleus pulposus of intervertebral discs

AU - Fujita, Nobuyuki

AU - Miyamoto, Takeshi

AU - Imai, Jun Ichi

AU - Hosogane, Naobumi

AU - Suzuki, Toru

AU - Yagi, Mitsuru

AU - Morita, Kozo

AU - Ninomiya, Ken

AU - Miyamoto, Kana

AU - Takaishi, Hironari

AU - Matsumoto, Morio

AU - Morioka, Hideo

AU - Yabe, Hiroo

AU - Chiba, Kazuhiro

AU - Watanabe, Shinya

AU - Toyama, Yoshiaki

AU - Suda, Toshio

PY - 2005/12/30

Y1 - 2005/12/30

N2 - Intervertebral disc (IVD) consists of a soft gelatinous material in its center, the nucleus pulposus (NP), bounded peripherally by fibrocartilage, annulus fibrosus (AF). Despite the number of patients with IVD degeneration, gene expression analysis has not been undertaken in NP and therefore little is known about the molecular markers expressed in NP. Here, we undertook a microarray screen in NP with the other nine tissues to identify the specific cell surface markers for NP. Five membrane associating molecules out of 10,490 genes were identified as highly expressing genes in NP compared with the other tissues. Among them, we identified CD24, a glycosylphosphatidylinositol (GPI) anchor protein as a cell surface marker for NP. CD24 expression was also detected in the herniated NP and chordoma, a malignant primary tumor derived from notochordal cells, while it was absent in chondrosarcoma. Therefore, CD24 is a molecular marker for NP as well as the diseases of IVD.

AB - Intervertebral disc (IVD) consists of a soft gelatinous material in its center, the nucleus pulposus (NP), bounded peripherally by fibrocartilage, annulus fibrosus (AF). Despite the number of patients with IVD degeneration, gene expression analysis has not been undertaken in NP and therefore little is known about the molecular markers expressed in NP. Here, we undertook a microarray screen in NP with the other nine tissues to identify the specific cell surface markers for NP. Five membrane associating molecules out of 10,490 genes were identified as highly expressing genes in NP compared with the other tissues. Among them, we identified CD24, a glycosylphosphatidylinositol (GPI) anchor protein as a cell surface marker for NP. CD24 expression was also detected in the herniated NP and chordoma, a malignant primary tumor derived from notochordal cells, while it was absent in chondrosarcoma. Therefore, CD24 is a molecular marker for NP as well as the diseases of IVD.

KW - CD24

KW - Intervertebral disc

KW - Nucleus pulposus

UR - http://www.scopus.com/inward/record.url?scp=27844438525&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27844438525&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2005.10.166

DO - 10.1016/j.bbrc.2005.10.166

M3 - Article

C2 - 16288985

AN - SCOPUS:27844438525

VL - 338

SP - 1890

EP - 1896

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -