CD3+ b-1a cells as a mediator of disease progression in autoimmune-prone mice

Wakako Yamamoto, Hidemi Toyoda, Dong Qing Xu, Ryo Hanaki, Mari Morimoto, Daisuke Nakato, Takahiro Ito, Shotaro Iwamoto, Motoki Bonno, Shigeki Tanaka, Masahiro Hirayama

Research output: Contribution to journalArticlepeer-review


B-1a cells are distinguishable from conventional B cells, which are designated B-2 cells, on the basis of their developmental origin, surface marker expression, and functions. In addition to the unique expression of the CD5 antigen, B-1a cells are characterized by the expression level of CD23. Although B-1a cells are considered to be independent of T cells and produce natural autoantibodies that induce the clinical manifestations of autoimmune diseases, there is much debate on the role of B-1a cells in the development of autoimmune diseases. We examined the involvement of B-1a cells in autoimmune-prone mice with the lpr gene. MRL/lpr and B6/lpr mice exhibited lupus and lymphoproliferative syndromes because of the massive accumulation of CD3+ CD4-CD8-B220+ T cells. Interestingly, the B220+CD23-CD5+ (B-1a) cell population in the peripheral blood and peritoneal cavity increased with age and disease progression. Ninety percent of B-1a cells were CD3 positive (CD3+ B-1a cells) and did not produce tumor necrosis factor alpha, interferon gamma, or interleukin-10. To test the possible involvement of CD3+ B-1a cells in autoimmune disease, we tried to eliminate the peripheral cells by hypotonic shock through repeated intraperitoneal injections of distilled water. The fraction of peritoneal CD3+ B-1a cells decreased, and symptoms of the autoimmune disease were much milder in the distilled water-treated MRL/lpr mice. These results suggest that CD3+ B-1a cells could be mediators of disease progression in autoimmune-prone mice.

Original languageEnglish
Article number9289417
JournalMediators of Inflammation
Publication statusPublished - 2018
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Cell Biology


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