CD300 antigen like family member G: A novel Ig receptor like protein exclusively expressed on capillary endothelium

Hiroyuki Takatsu, Kouji Hase, Masumi Ohmae, Sayaka Ohshima, Koji Hashimoto, Naoko Taniura, Akitsugu Yamamoto, Hiroshi Ohno

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We report the characteristics of CD300LG, a member of the CD300 antigen like family. Its genomic structure is similar in both mouse and human, and at least four isoforms exist in both species. The amino acid sequence of the immunoglobulin (Ig) V like domain of CD300LG showed approximately 35% identity to those of the polymeric Ig receptor (pIgR) and Fcα/μR. Interestingly, mouse CD300LG proteins were uniquely expressed on capillary endothelium. Immunoelectron microscopy revealed that mouse CD300LG is localized on both apical and basolateral plasma membranes, as well as on intracellular vesicular structures, in the capillary endothelium. Transcytosis assays using polarized MDCK epithelial cells showed that CD300LG could be transcytosed bidirectionally. Furthermore, CD300LG exogenously expressed on HeLa cells could take up IgA2 and IgM, but not IgG. These results suggest that CD300LG might play an important role in molecular traffic across the capillary endothelium.

Original languageEnglish
Pages (from-to)183-191
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume348
Issue number1
DOIs
Publication statusPublished - 2006 Sep 15
Externally publishedYes

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Keywords

  • Capillary endothelium
  • CD300L
  • CMRF35
  • IgA
  • IgM
  • Immunoglobulin super family
  • Transcytosis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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