CD9 correlates with cancer stem cell potentials in human B-acute lymphoblastic leukemia cells

Hiroko Nishida, Hiroto Yamazaki, Taketo Yamada, Satoshi Iwata, Nam H. Dang, Takeshi Inukai, Kanji Sugita, Yasuo Ikeda, Chikao Morimoto

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Cancer stem cell (CSC) theory suggests that only a small subpopulation of cells having stem cell-like potentials can initiate tumor development. While recent data on acute lymphoblastic leukemia (ALL) are conflicting, some studies have demonstrated the existence of such cells following CD34-targeted isolation of primary samples. Although CD34 is a useful marker for the isolation of CSCs in leukemias, the identification of other specific markers besides CD34 has been relatively unsuccessful. To identify new markers, we first performed extensive analysis of surface markers on several B-ALL cell lines. Our data demonstrated that every B-ALL cell line tested did not express CD34 but certain lines contained cell populations with marked heterogeneity in marker expression. Moreover, the CD9+ cell population possessed stem cell characteristics within the clone, as demonstrated by in vitro and transplantation experiments. These results suggest that CD9 is a useful positive-selection marker for the identification of CSCs in B-ALL.

Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume382
Issue number1
DOIs
Publication statusPublished - 2009 Apr 24
Externally publishedYes

Keywords

  • B-acute lymphoblastic leukemia
  • CD9
  • Cancer stem cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Nishida, H., Yamazaki, H., Yamada, T., Iwata, S., Dang, N. H., Inukai, T., Sugita, K., Ikeda, Y., & Morimoto, C. (2009). CD9 correlates with cancer stem cell potentials in human B-acute lymphoblastic leukemia cells. Biochemical and Biophysical Research Communications, 382(1), 57-62. https://doi.org/10.1016/j.bbrc.2009.02.123