Cdc42 has important roles in postnatal angiogenesis and vasculature formation

Yuko Yoshida, Atsushi Yamada, Yoshihiro Akimoto, Kyoko Abe, Sachie Matsubara, Junri Hayakawa, Junichi Tanaka, Mitsuhiro Kinoshita, Tadashi Kato, Hiroaki Ogata, Akiko Sakashita, Kenji Mishima, Yoshiaki Kubota, Hayato Kawakami, Ryutaro Kamijo, Takehiko Iijima

Research output: Contribution to journalArticlepeer-review

Abstract

Cdc42, a Rho family low molecular weight G protein, has important roles in various cell functions, including cytoskeletal rearrangement, cell adhesion and cell proliferation and differentiation. To investigate the involvement of Cdc42 in the activities of vascular endothelial cells, we generated Cdc42 conditional knockout mice in which Cdc42 was time -specifically deficient in vascular endothelial cells (Cdc42 ​fl/fl; VE-Cad CreERT: Cdc42 cKO). When the Cdc42 gene was deleted after birth, Cdc42 cKO mice were smaller than the control mice, and died between postnatal day 8 (P8) and P10. Necropsy findings confirmed that these mice had various pathological aberrances in the vessels of most organs, such as blood flow congestion and blood cell invasion. Electron microscopic observations also revealed that capillary endothelial cells were detached from the basement membrane as well as phagocytosis of dead endothelial cells induced by macrophages. Moreover, vascular sprouting from aortic rings induced by VEGF-A was diminished in samples from the Cdc42 cKO mice because of an endothelial cell proliferation defect. These results suggest that Cdc42 in vascular endothelial cells has important roles in blood vessel formation after birth.

Original languageEnglish
Pages (from-to)64-69
Number of pages6
JournalDevelopmental Biology
Volume477
DOIs
Publication statusPublished - 2021 Sep

Keywords

  • Angiogenesis
  • Cdc42
  • Congestion
  • Rho GTPase
  • VE-Cadherin

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Cdc42 has important roles in postnatal angiogenesis and vasculature formation'. Together they form a unique fingerprint.

Cite this