cDNA cloning and chromosomal localization of the human ciliary neurotrophic factor gene

Hidehiro Yokoji; Takeshi Ariyama; Ryosuke Takahashi; Johji Inazawa; Hidemi Misawa; Takeo Deguchi

doi:10.1016/0304-3940(95)11254-T

cDNA cloning and chromosomal localization of the human ciliary neurotrophic factor gene

Hidehiro Yokoji, Takeshi Ariyama, Ryosuke Takahashi, Johji Inazawa, Hidemi Misawa, Takeo Deguchi

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Full-length cDNA for human ciliary neurotrophic factor (CNTF) was isolated from a human sciatic nerve cDNA library. Sequence analysis revealed that the longest cDNA was comprised of a 48-bp 5′-untranslated region, a 600-bp coding region and a 1207-bp 3′-untranslated region containing four ATTA pentamer motifs and a polyadenylation sequence. The transcription starting point was assigned at 81 bp upstream of the initiation methionine by 5′ RACE analysis. Using the cDNA and genomic DNA fragment including the entire intron region as mixed probes, the human CNTF gene was localized to the long arm of chromosome 11 at region q12 by fluorescence in situ hybridization.

Original language	English
Pages (from-to)	175-178
Number of pages	4
Journal	Neuroscience Letters
Volume	185
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(95)11254-T
Publication status	Published - 1995 Feb 13
Externally published	Yes

Keywords

Chromosomal localization
Fluorescence in situ hybridization
Gene structure
Human ciliary neurotrophic factor
Transcription starting point
cDNA

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0304-3940(95)11254-T

Cite this

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title = "cDNA cloning and chromosomal localization of the human ciliary neurotrophic factor gene",

abstract = "Full-length cDNA for human ciliary neurotrophic factor (CNTF) was isolated from a human sciatic nerve cDNA library. Sequence analysis revealed that the longest cDNA was comprised of a 48-bp 5′-untranslated region, a 600-bp coding region and a 1207-bp 3′-untranslated region containing four ATTA pentamer motifs and a polyadenylation sequence. The transcription starting point was assigned at 81 bp upstream of the initiation methionine by 5′ RACE analysis. Using the cDNA and genomic DNA fragment including the entire intron region as mixed probes, the human CNTF gene was localized to the long arm of chromosome 11 at region q12 by fluorescence in situ hybridization.",

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T1 - cDNA cloning and chromosomal localization of the human ciliary neurotrophic factor gene

AU - Yokoji, Hidehiro

AU - Ariyama, Takeshi

AU - Takahashi, Ryosuke

AU - Inazawa, Johji

AU - Misawa, Hidemi

AU - Deguchi, Takeo

PY - 1995/2/13

Y1 - 1995/2/13

N2 - Full-length cDNA for human ciliary neurotrophic factor (CNTF) was isolated from a human sciatic nerve cDNA library. Sequence analysis revealed that the longest cDNA was comprised of a 48-bp 5′-untranslated region, a 600-bp coding region and a 1207-bp 3′-untranslated region containing four ATTA pentamer motifs and a polyadenylation sequence. The transcription starting point was assigned at 81 bp upstream of the initiation methionine by 5′ RACE analysis. Using the cDNA and genomic DNA fragment including the entire intron region as mixed probes, the human CNTF gene was localized to the long arm of chromosome 11 at region q12 by fluorescence in situ hybridization.

AB - Full-length cDNA for human ciliary neurotrophic factor (CNTF) was isolated from a human sciatic nerve cDNA library. Sequence analysis revealed that the longest cDNA was comprised of a 48-bp 5′-untranslated region, a 600-bp coding region and a 1207-bp 3′-untranslated region containing four ATTA pentamer motifs and a polyadenylation sequence. The transcription starting point was assigned at 81 bp upstream of the initiation methionine by 5′ RACE analysis. Using the cDNA and genomic DNA fragment including the entire intron region as mixed probes, the human CNTF gene was localized to the long arm of chromosome 11 at region q12 by fluorescence in situ hybridization.

KW - Chromosomal localization

KW - Fluorescence in situ hybridization

KW - Gene structure

KW - Human ciliary neurotrophic factor

KW - Transcription starting point

KW - cDNA

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cDNA cloning and chromosomal localization of the human ciliary neurotrophic factor gene

Abstract

Keywords

ASJC Scopus subject areas

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