cDNA cloning and chromosomal localization of the human ciliary neurotrophic factor gene

Hidehiro Yokoji, Takeshi Ariyama, Ryosuke Takahashi, Johji Inazawa, Hidemi Misawa, Takeo Deguchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Full-length cDNA for human ciliary neurotrophic factor (CNTF) was isolated from a human sciatic nerve cDNA library. Sequence analysis revealed that the longest cDNA was comprised of a 48-bp 5′-untranslated region, a 600-bp coding region and a 1207-bp 3′-untranslated region containing four ATTA pentamer motifs and a polyadenylation sequence. The transcription starting point was assigned at 81 bp upstream of the initiation methionine by 5′ RACE analysis. Using the cDNA and genomic DNA fragment including the entire intron region as mixed probes, the human CNTF gene was localized to the long arm of chromosome 11 at region q12 by fluorescence in situ hybridization.

Original languageEnglish
Pages (from-to)175-178
Number of pages4
JournalNeuroscience Letters
Volume185
Issue number3
DOIs
Publication statusPublished - 1995 Feb 13
Externally publishedYes

Keywords

  • Chromosomal localization
  • Fluorescence in situ hybridization
  • Gene structure
  • Human ciliary neurotrophic factor
  • Transcription starting point
  • cDNA

ASJC Scopus subject areas

  • Neuroscience(all)

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