cDNA cloning, characterization, and functional expression of 4S-(-)- limonene synthase from Perilia frutescens

Akiko Yuba, Kazufumi Yazaki, Mamoru Tabata, Gisho Honda, Rodney Croteau

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48 Citations (Scopus)


A molecular biological study on limonene synthase that catalyzes the cyclization of geranyldiphosphate to yield the olefin 4(S)-limonene, an intermediate in the biosynthesis of a monoterpenoid, perillaldehyde, in Perilla frutescens Britton has been carried out. We isolated and characterized 10 cDNAs homologous to spearmint limonene synthase cDNA from an expression library constructed from cotyledons of a Perilla strain homozygous for two pairs of dominant genes, G and H, which are responsible for the formation of 4(S)-limonene. Two of these cDNA clones were functionally expressed in Escherichia coli, yielding enzymes which were catalytically active in generating 4(S)-limonene from geranyldiphosphate. The longest open reading frame in the representative cDNA clone PFLC1 consisted of 1812 nucleotides corresponding to 603 amino acids. Its identity to the ORFs of spearmint limonene synthase, tobacco 5-epi-aristolochene synthase, and castor bean casbene synthase were 65, 35, and 30%, respectively. Genomic Southern blot analyses of various genotypes (GGHH, GGhh, ggHH, and gghh) of P. frutescens suggested that more than one copy of the PFLC1 DNA exists in strains having the HH genotype. In contrast, no PFLC1 DNA sequences were found in the genomes of strains with the hh genotype that are incapable of producing cyclohexanoid monoterpenes for lack of limonene synthase activity. Northern blot analyses, using a PFLC13'-flanking region as a hybridization probe, showed that PFLC1 mRNA accumulated in all the aerial parts of the GGHH plants, particularly in the leaves. In the ggHH plants, on the other hand, PFLC1 mRNA was detected only in minute amounts in the stem and calyx.

Original languageEnglish
Pages (from-to)280-287
Number of pages8
JournalArchives of Biochemistry and Biophysics
Issue number2
Publication statusPublished - 1996 Aug 15
Externally publishedYes


  • Perilla frutescens
  • genetic analysis
  • l-limonene
  • limonene synthase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology


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