cDNA cloning of Na+, K+-ATPase α-subunit from embryos of the sea urchin, Hemicentrotus pulcherrimus

Keiko Mitsunaga-Nakatsubo, Ken Yamazaki, Midori Hatoh-Okazaki, Hiroto Kawashita, Chisato Okamura, Koji Akasaka, Hiraku Shimada, Ikuo Yasumasu

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7 Citations (Scopus)

Abstract

Na+, K+-ATPase α-subunit cDNA of the sea urchin, Hemicentrotus pulcherrimus, was obtained by twice screening prism and gastrula λgt10 cDNA libraries using an oligonucleotide probe derived from a mostly conserved region, FSBA (5′-p-(fluorosulfonyl)-benzoyladenosine) binding site of cation transport ATPases. The 5′-end of the non-coding region was determined by primer extension and the region was amplified by 5′-RACE method. The sea urchin α-subunit cDNA consists of 4401 nucleotides and encodes 1038 amino acid residues (MW, 114 kDa). The predicted primary structure, except N-terminal region, has similar degree of high homology to various metazoan Na+, K+-ATPase α-subunits. Alignment of amino acid sequence and a hydropathy profile also predicts eight putative transmembrane segments at least. The phylogenetic tree suspected from alignment of amino acid sequences of 21 species suggests that sea urchin and vertebrate Na+, K+-ATPase α-subunits seem to have evolved from a common origin, before vertebrate α-subunit divided into three isoforms.

Original languageEnglish
Pages (from-to)833-841
Number of pages9
JournalZoological Science
Volume13
Issue number6
DOIs
Publication statusPublished - 1996 Dec

ASJC Scopus subject areas

  • Animal Science and Zoology

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    Mitsunaga-Nakatsubo, K., Yamazaki, K., Hatoh-Okazaki, M., Kawashita, H., Okamura, C., Akasaka, K., Shimada, H., & Yasumasu, I. (1996). cDNA cloning of Na+, K+-ATPase α-subunit from embryos of the sea urchin, Hemicentrotus pulcherrimus. Zoological Science, 13(6), 833-841. https://doi.org/10.2108/zsj.13.833