cDNA Expression Cloning of the 85-kDa Protein Overexpressed in Adriamycin-Resistant Cells

Kohji Noguchi, M. Naito, K. Tezuka, S. Ishii, H. Seimiya, Yoshikazu Sugimoto, E. Amann, T. Tsuruo

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

An 85-kDa cell surface protein recognized by monoclonal antibody MRK-20 was identified in adriamycin-resistant tumor cells. The expression of the 85-kDa protein has recently been reported to be associated with the differentiation of hematopoietic cells. However, the primary structure of the 85-kDa protein has not been determined. To clarify its primary structure, we carried out cDNA expression cloning of the 85-kDa protein with monoclonal antibody MRK-20. We found that the 85-kDa protein is identical to CD36 (GP VI), but at least two species of transcript exist in the tumor cells and one of these transcripts has a novel sequence at the 3′-region. The transcript with the novel sequence at the 3′-region was found to be expressed during the differentiation of hematopoietic cells.

Original languageEnglish
Pages (from-to)88-95
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume192
Issue number1
DOIs
Publication statusPublished - 1993 Apr 15
Externally publishedYes

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Cloning
Doxorubicin
Organism Cloning
Complementary DNA
Cells
Tumors
Cell Differentiation
Proteins
Monoclonal Antibodies
Neoplasms
Membrane Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

cDNA Expression Cloning of the 85-kDa Protein Overexpressed in Adriamycin-Resistant Cells. / Noguchi, Kohji; Naito, M.; Tezuka, K.; Ishii, S.; Seimiya, H.; Sugimoto, Yoshikazu; Amann, E.; Tsuruo, T.

In: Biochemical and Biophysical Research Communications, Vol. 192, No. 1, 15.04.1993, p. 88-95.

Research output: Contribution to journalArticle

Noguchi, Kohji ; Naito, M. ; Tezuka, K. ; Ishii, S. ; Seimiya, H. ; Sugimoto, Yoshikazu ; Amann, E. ; Tsuruo, T. / cDNA Expression Cloning of the 85-kDa Protein Overexpressed in Adriamycin-Resistant Cells. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 192, No. 1. pp. 88-95.
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