cDNA expression cloning of the 85-kDa protein overexpressed in adriamycin-resistant cells

Kohji Noguchi, Mikihiko Naito, Ken Ichi Tezuka, Shigetaka Ishii, Hiroyuki Seimiya, Yoshikazu Sugimoto, Egon Amann, Takashi Tsuruo

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

An 85-kDa cell surface protein recognized by monoclonal antibody MRK-20 was identified in adriamycin-resistant tumor cells. The expression of the 85-kDa protein has recently been reported to be associated with the differentiation of hematopoietic cells. However, the primary structure of the 85-kDa protein has not been determined. To clarify its primary structure, we carried out cDNA expression cloning of the 85-kDa protein with monoclonal antibody MRK-20. We found that the 85-kDa protein is identical to CD36 (GP VI), but at least two species of transcript exist in the tumor cells and one of these transcripts has a novel sequence at the 3′-region. The transcript with the novel sequence at the 3′-region was found to be expressed during the differentiation of hematopoietic cells.

Original languageEnglish
Pages (from-to)88-95
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume192
Issue number1
DOIs
Publication statusPublished - 1993 Apr 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'cDNA expression cloning of the 85-kDa protein overexpressed in adriamycin-resistant cells'. Together they form a unique fingerprint.

  • Cite this