Cds nanoparticle-based biosensor development for aflatoxin determination

Moh Hayat, Endang Saepudin, Yasuaki Einaga, Tribidasari A. Ivandini

Research output: Contribution to journalArticle

Abstract

An anodic stripping voltammetry detection method with lateral flow immunochromatographic assay for determining aflatoxin M1 was developed. Cadmium sulfide nanoparticles (CdSNPs) were employed as a label for aflatoxin antibody. Conjugation between the CdSNPs and aflatoxin antibody was performed as the first step. The CdSNP-antibody conjugate was then immobilized on a test strip on a conjugate pad. When the conjugate interacted with the sample containing aflatoxin, it was captured by the aflatoxin in the test zone. The CdSNPs retained in the test zone were then measured by anodic stripping voltammetry using a boron-doped diamond electrode. A linear concentration range of 0-70 ppb aflatoxin, with a sensitivity of 20 μA/mM and a detection limit of 30 ppb, was achieved by this method.

Original languageEnglish
Pages (from-to)787-797
Number of pages11
JournalInternational Journal of Technology
Volume10
Issue number4
DOIs
Publication statusPublished - 2019 Jul 1

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Keywords

  • Aflatoxin
  • Anodic stripping voltammetry
  • Boron-doped diamond
  • CdS nanoparticles

ASJC Scopus subject areas

  • Engineering(all)
  • Strategy and Management
  • Management of Technology and Innovation

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