Cell adherence-promoted activity of Plesiomonas shigelloides GroEL

Hitoshi Tsugawa, Humie Ito, Miho Ohshima, Yoshio Okawa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Previously, it has been demonstrated that the invasion of Caco-2 cells by Plesiomonas shigelloides induces apoptotic cell death. Therefore, the attachment to and colonization of eukaryotic intestinal host cells by P. shigelloides are important steps in causing pathogenicity. In this study, the participation of P. shigelloides GroEL in the attachment of P. shigelloides was examined. The groESL operon of P. shigelloides was isolated by PCR. The nucleotide sequence of the groESL operon of P. shigelloides revealed two ORFs of 294 nucleotides for groES and 1647 nucleotides for groEL. Cell fractionation and immunostaining experiments suggested that the GroEL of P. shigelloides was associated with the bacterial cell surface. The expression of the groEL gene was upregulated during the attachment and apoptosis-induction stages, and the expression of the protein was also induced during the attachment stage. Furthermore, GroEL efficiently promoted the attachment of P. shigelloides to Caco-2 cells, as measured by a FACSCalibur flow cytometer. These results demonstrated that GroEL has a positive influence on the attachment of P. shigelloides to Caco-2 cells.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalJournal of Medical Microbiology
Volume56
Issue number1
DOIs
Publication statusPublished - 2007 Jan
Externally publishedYes

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Plesiomonas
Caco-2 Cells
Operon
Nucleotides
Cell Fractionation
Open Reading Frames
Virulence
Cell Death

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Cell adherence-promoted activity of Plesiomonas shigelloides GroEL. / Tsugawa, Hitoshi; Ito, Humie; Ohshima, Miho; Okawa, Yoshio.

In: Journal of Medical Microbiology, Vol. 56, No. 1, 01.2007, p. 23-29.

Research output: Contribution to journalArticle

Tsugawa, Hitoshi ; Ito, Humie ; Ohshima, Miho ; Okawa, Yoshio. / Cell adherence-promoted activity of Plesiomonas shigelloides GroEL. In: Journal of Medical Microbiology. 2007 ; Vol. 56, No. 1. pp. 23-29.
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