Cell adhesion of morphology in porous scaffold based on enantiomeric poly(lactic acid) graft-type phospholipid polymers

Junji Watanabe, Takahisa Eriguchi, Kazuhiko Ishihara

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Poly(D-lactic acid) (PDLA) and poly(L-lactic acid) (PLLA) macromonomers were synthesized for preparation of a novel cytocompatible polymer. The cytocompatible polymer was composed of 2-methacryloyloxyethyl phosphorylcholine (MPC), n-butyl methacrylate (BMA), and the enantiomeric PLLA (or PDLA) macromonomer. The degree of polymerization of the lactic acid in the PLLA and PDLA segments was designed to be ca. 20. The copolymer-coated surface was analyzed with static contact angle by water. From the result, the PLLA (or PDLA) segment and MPC unit were located on the coated surface, and the monomer unit in the copolymer was reconstructed by contacting water. Fibroblast cell culture was performed to evaluate cell adhesion on the coated surface, and the cell morphology was observed. The number of cell adhesion is correlated with the PL(D)LA content, and the cell morphology is correlated with the MPC unit content. The porous scaffold was prepared by the formation of a stereocomplex between the PLLA and PDLA, and the cell adhesion and following cell intrusion was then evaluated. The fibroblast cells adhered on the surface and intruded into the scaffold through the connecting pores after 24 h. The cell morphology became round shape from spreading with the decreasing PLLA (or PDLA) content in the copolymer. It is considered that the change in the cell morphology would be induced by the MPC unit as cytocompatible unit. These findings suggest that the porous scaffold makes it possible to have cytocompatibility and to produce three-dimensional tissue regeneration.

Original languageEnglish
Pages (from-to)1375-1383
Number of pages9
JournalBiomacromolecules
Volume3
Issue number6
DOIs
Publication statusPublished - 2002 Nov
Externally publishedYes

Fingerprint

Cell adhesion
Phospholipids
Lactic acid
Scaffolds (biology)
Cell Adhesion
Grafts
Lactic Acid
Polymers
Transplants
Phosphorylcholine
Scaffolds
Copolymers
Fibroblasts
Tissue regeneration
Water
poly(lactic acid)
Cell culture
Contact angle
Monomers
Polymerization

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Cell adhesion of morphology in porous scaffold based on enantiomeric poly(lactic acid) graft-type phospholipid polymers. / Watanabe, Junji; Eriguchi, Takahisa; Ishihara, Kazuhiko.

In: Biomacromolecules, Vol. 3, No. 6, 11.2002, p. 1375-1383.

Research output: Contribution to journalArticle

Watanabe, Junji ; Eriguchi, Takahisa ; Ishihara, Kazuhiko. / Cell adhesion of morphology in porous scaffold based on enantiomeric poly(lactic acid) graft-type phospholipid polymers. In: Biomacromolecules. 2002 ; Vol. 3, No. 6. pp. 1375-1383.
@article{f6aa060f81664f84a56360d309211c34,
title = "Cell adhesion of morphology in porous scaffold based on enantiomeric poly(lactic acid) graft-type phospholipid polymers",
abstract = "Poly(D-lactic acid) (PDLA) and poly(L-lactic acid) (PLLA) macromonomers were synthesized for preparation of a novel cytocompatible polymer. The cytocompatible polymer was composed of 2-methacryloyloxyethyl phosphorylcholine (MPC), n-butyl methacrylate (BMA), and the enantiomeric PLLA (or PDLA) macromonomer. The degree of polymerization of the lactic acid in the PLLA and PDLA segments was designed to be ca. 20. The copolymer-coated surface was analyzed with static contact angle by water. From the result, the PLLA (or PDLA) segment and MPC unit were located on the coated surface, and the monomer unit in the copolymer was reconstructed by contacting water. Fibroblast cell culture was performed to evaluate cell adhesion on the coated surface, and the cell morphology was observed. The number of cell adhesion is correlated with the PL(D)LA content, and the cell morphology is correlated with the MPC unit content. The porous scaffold was prepared by the formation of a stereocomplex between the PLLA and PDLA, and the cell adhesion and following cell intrusion was then evaluated. The fibroblast cells adhered on the surface and intruded into the scaffold through the connecting pores after 24 h. The cell morphology became round shape from spreading with the decreasing PLLA (or PDLA) content in the copolymer. It is considered that the change in the cell morphology would be induced by the MPC unit as cytocompatible unit. These findings suggest that the porous scaffold makes it possible to have cytocompatibility and to produce three-dimensional tissue regeneration.",
author = "Junji Watanabe and Takahisa Eriguchi and Kazuhiko Ishihara",
year = "2002",
month = "11",
doi = "10.1021/bm025652p",
language = "English",
volume = "3",
pages = "1375--1383",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Cell adhesion of morphology in porous scaffold based on enantiomeric poly(lactic acid) graft-type phospholipid polymers

AU - Watanabe, Junji

AU - Eriguchi, Takahisa

AU - Ishihara, Kazuhiko

PY - 2002/11

Y1 - 2002/11

N2 - Poly(D-lactic acid) (PDLA) and poly(L-lactic acid) (PLLA) macromonomers were synthesized for preparation of a novel cytocompatible polymer. The cytocompatible polymer was composed of 2-methacryloyloxyethyl phosphorylcholine (MPC), n-butyl methacrylate (BMA), and the enantiomeric PLLA (or PDLA) macromonomer. The degree of polymerization of the lactic acid in the PLLA and PDLA segments was designed to be ca. 20. The copolymer-coated surface was analyzed with static contact angle by water. From the result, the PLLA (or PDLA) segment and MPC unit were located on the coated surface, and the monomer unit in the copolymer was reconstructed by contacting water. Fibroblast cell culture was performed to evaluate cell adhesion on the coated surface, and the cell morphology was observed. The number of cell adhesion is correlated with the PL(D)LA content, and the cell morphology is correlated with the MPC unit content. The porous scaffold was prepared by the formation of a stereocomplex between the PLLA and PDLA, and the cell adhesion and following cell intrusion was then evaluated. The fibroblast cells adhered on the surface and intruded into the scaffold through the connecting pores after 24 h. The cell morphology became round shape from spreading with the decreasing PLLA (or PDLA) content in the copolymer. It is considered that the change in the cell morphology would be induced by the MPC unit as cytocompatible unit. These findings suggest that the porous scaffold makes it possible to have cytocompatibility and to produce three-dimensional tissue regeneration.

AB - Poly(D-lactic acid) (PDLA) and poly(L-lactic acid) (PLLA) macromonomers were synthesized for preparation of a novel cytocompatible polymer. The cytocompatible polymer was composed of 2-methacryloyloxyethyl phosphorylcholine (MPC), n-butyl methacrylate (BMA), and the enantiomeric PLLA (or PDLA) macromonomer. The degree of polymerization of the lactic acid in the PLLA and PDLA segments was designed to be ca. 20. The copolymer-coated surface was analyzed with static contact angle by water. From the result, the PLLA (or PDLA) segment and MPC unit were located on the coated surface, and the monomer unit in the copolymer was reconstructed by contacting water. Fibroblast cell culture was performed to evaluate cell adhesion on the coated surface, and the cell morphology was observed. The number of cell adhesion is correlated with the PL(D)LA content, and the cell morphology is correlated with the MPC unit content. The porous scaffold was prepared by the formation of a stereocomplex between the PLLA and PDLA, and the cell adhesion and following cell intrusion was then evaluated. The fibroblast cells adhered on the surface and intruded into the scaffold through the connecting pores after 24 h. The cell morphology became round shape from spreading with the decreasing PLLA (or PDLA) content in the copolymer. It is considered that the change in the cell morphology would be induced by the MPC unit as cytocompatible unit. These findings suggest that the porous scaffold makes it possible to have cytocompatibility and to produce three-dimensional tissue regeneration.

UR - http://www.scopus.com/inward/record.url?scp=0036859593&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036859593&partnerID=8YFLogxK

U2 - 10.1021/bm025652p

DO - 10.1021/bm025652p

M3 - Article

VL - 3

SP - 1375

EP - 1383

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 6

ER -