Cell-cycle-specific nestin expression coordinates with morphological changes in embryonic cortical neural progenitors

Takehiko Sunabori, Akinori Tokunaga, Takeharu Nagai, Kazunobu Sawamoto, Masaru Okabe, Atsushi Miyawaki, Yumi Matsuzaki, Takaki Miyata, Hideyuki Okano

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

During brain development, neural progenitor cells extend across the thickening brain wall and undergo mitosis. To understand how these two completely different cellular events are coordinated, we focused on the transcription pattern of the nestin gene (Nes), which encodes an intermediate filament protein strongly expressed in neural progenitor cells. To visualize nestin expression in vivo, we generated transgenic mice that expressed a destabilized fluorescent protein under the control of Nes second intronic enhancer (E/nestin:dVenus). During the neurogenic stage, when the brain wall thickens markedly, we found that nestin was regulated in a cell-cycle-dependent manner. Time-lapse imaging showed that nestin gene expression was upregulated during G1-S phase, when the neural progenitor cells elongate their fibers. However, nestin expression dramatically declined in G2-M phase, when progenitor cells round up to undergo mitosis. The cell-cycle-dependent phosphorylation of an upstream regulator class III POU transcription factor (Pou3f2 or Brn2) reduced its binding activity to the nestin core enhancer element and was therefore responsible for the decreased Nes transcription in G2-M phase. Collectively, these findings demonstrate precisely orchestrated gene regulation that correlates with the 3D morphological changes in neural progenitor cells in vivo.

Original languageEnglish
Pages (from-to)1204-1212
Number of pages9
JournalJournal of Cell Science
Volume121
Issue number8
DOIs
Publication statusPublished - 2008 Apr 15

Fingerprint

Nestin
Cell Cycle
Stem Cells
G2 Phase
Mitosis
Cell Division
Brain
POU Domain Factors
Time-Lapse Imaging
Intermediate Filament Proteins
G1 Phase
S Phase
Transgenic Mice
Genes
Phosphorylation
Gene Expression

Keywords

  • Cell cycle
  • Intermediate filament
  • Phosphorylation
  • POU
  • Radial glia
  • SOX

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cell-cycle-specific nestin expression coordinates with morphological changes in embryonic cortical neural progenitors. / Sunabori, Takehiko; Tokunaga, Akinori; Nagai, Takeharu; Sawamoto, Kazunobu; Okabe, Masaru; Miyawaki, Atsushi; Matsuzaki, Yumi; Miyata, Takaki; Okano, Hideyuki.

In: Journal of Cell Science, Vol. 121, No. 8, 15.04.2008, p. 1204-1212.

Research output: Contribution to journalArticle

Sunabori, T, Tokunaga, A, Nagai, T, Sawamoto, K, Okabe, M, Miyawaki, A, Matsuzaki, Y, Miyata, T & Okano, H 2008, 'Cell-cycle-specific nestin expression coordinates with morphological changes in embryonic cortical neural progenitors', Journal of Cell Science, vol. 121, no. 8, pp. 1204-1212. https://doi.org/10.1242/jcs.025064
Sunabori, Takehiko ; Tokunaga, Akinori ; Nagai, Takeharu ; Sawamoto, Kazunobu ; Okabe, Masaru ; Miyawaki, Atsushi ; Matsuzaki, Yumi ; Miyata, Takaki ; Okano, Hideyuki. / Cell-cycle-specific nestin expression coordinates with morphological changes in embryonic cortical neural progenitors. In: Journal of Cell Science. 2008 ; Vol. 121, No. 8. pp. 1204-1212.
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