Abstract
Engineering an efficient biomolecular circuit often requires time-consuming iterations of optimization. Cell-free protein expression systems allow rapid testing of biocircuits in vitro, speeding the design-build-test cycle of synthetic biology. In this paper, we combine this with droplet microfluidics to densely scan a transcription-translation biocircuit space. Our system assays millions of parameter combinations per hour, providing a detailed map of function. The ability to comprehensively map biocircuit parameter spaces allows accurate modeling to predict circuit function and identify optimal circuits and conditions.
Original language | English |
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Pages (from-to) | 3037-3042 |
Number of pages | 6 |
Journal | Lab on a Chip |
Volume | 17 |
Issue number | 18 |
DOIs | |
Publication status | Published - 2017 Sep 21 |
Externally published | Yes |
ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- Chemistry(all)
- Biomedical Engineering