Cell-free extract based optimization of biomolecular circuits with droplet microfluidics

Yutaka Hori, Chaitanya Kantak, Richard M. Murray, Adam R. Abate

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Engineering an efficient biomolecular circuit often requires time-consuming iterations of optimization. Cell-free protein expression systems allow rapid testing of biocircuits in vitro, speeding the design-build-test cycle of synthetic biology. In this paper, we combine this with droplet microfluidics to densely scan a transcription-translation biocircuit space. Our system assays millions of parameter combinations per hour, providing a detailed map of function. The ability to comprehensively map biocircuit parameter spaces allows accurate modeling to predict circuit function and identify optimal circuits and conditions.

Original languageEnglish
Pages (from-to)3037-3042
Number of pages6
JournalLab on a Chip
Volume17
Issue number18
DOIs
Publication statusPublished - 2017 Jan 1
Externally publishedYes

Fingerprint

Synthetic Biology
Microfluidics
Cell Extracts
Networks (circuits)
Proteins
Transcription
Assays
Testing
In Vitro Techniques

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Cell-free extract based optimization of biomolecular circuits with droplet microfluidics. / Hori, Yutaka; Kantak, Chaitanya; Murray, Richard M.; Abate, Adam R.

In: Lab on a Chip, Vol. 17, No. 18, 01.01.2017, p. 3037-3042.

Research output: Contribution to journalArticle

Hori, Yutaka ; Kantak, Chaitanya ; Murray, Richard M. ; Abate, Adam R. / Cell-free extract based optimization of biomolecular circuits with droplet microfluidics. In: Lab on a Chip. 2017 ; Vol. 17, No. 18. pp. 3037-3042.
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