Abstract
Engineering an efficient biomolecular circuit often requires time-consuming iterations of optimization. Cell-free protein expression systems allow rapid testing of biocircuits in vitro, speeding the design-build-test cycle of synthetic biology. In this paper, we combine this with droplet microfluidics to densely scan a transcription-translation biocircuit space. Our system assays millions of parameter combinations per hour, providing a detailed map of function. The ability to comprehensively map biocircuit parameter spaces allows accurate modeling to predict circuit function and identify optimal circuits and conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 3037-3042 |
| Number of pages | 6 |
| Journal | Lab on a Chip |
| Volume | 17 |
| Issue number | 18 |
| DOIs | |
| Publication status | Published - 2017 Jan 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- Chemistry(all)
- Biomedical Engineering
Cite this
Cell-free extract based optimization of biomolecular circuits with droplet microfluidics. / Hori, Yutaka; Kantak, Chaitanya; Murray, Richard M.; Abate, Adam R.
In: Lab on a Chip, Vol. 17, No. 18, 01.01.2017, p. 3037-3042.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell-free extract based optimization of biomolecular circuits with droplet microfluidics
AU - Hori, Yutaka
AU - Kantak, Chaitanya
AU - Murray, Richard M.
AU - Abate, Adam R.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Engineering an efficient biomolecular circuit often requires time-consuming iterations of optimization. Cell-free protein expression systems allow rapid testing of biocircuits in vitro, speeding the design-build-test cycle of synthetic biology. In this paper, we combine this with droplet microfluidics to densely scan a transcription-translation biocircuit space. Our system assays millions of parameter combinations per hour, providing a detailed map of function. The ability to comprehensively map biocircuit parameter spaces allows accurate modeling to predict circuit function and identify optimal circuits and conditions.
AB - Engineering an efficient biomolecular circuit often requires time-consuming iterations of optimization. Cell-free protein expression systems allow rapid testing of biocircuits in vitro, speeding the design-build-test cycle of synthetic biology. In this paper, we combine this with droplet microfluidics to densely scan a transcription-translation biocircuit space. Our system assays millions of parameter combinations per hour, providing a detailed map of function. The ability to comprehensively map biocircuit parameter spaces allows accurate modeling to predict circuit function and identify optimal circuits and conditions.
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UR - http://www.scopus.com/inward/citedby.url?scp=85029374861&partnerID=8YFLogxK
U2 - 10.1039/c7lc00552k
DO - 10.1039/c7lc00552k
M3 - Article
VL - 17
SP - 3037
EP - 3042
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
SN - 1473-0197
IS - 18
ER -