Cell-free extract based optimization of biomolecular circuits with droplet microfluidics

Yutaka Hori, Chaitanya Kantak, Richard M. Murray, Adam R. Abate

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Engineering an efficient biomolecular circuit often requires time-consuming iterations of optimization. Cell-free protein expression systems allow rapid testing of biocircuits in vitro, speeding the design-build-test cycle of synthetic biology. In this paper, we combine this with droplet microfluidics to densely scan a transcription-translation biocircuit space. Our system assays millions of parameter combinations per hour, providing a detailed map of function. The ability to comprehensively map biocircuit parameter spaces allows accurate modeling to predict circuit function and identify optimal circuits and conditions.

Original languageEnglish
Pages (from-to)3037-3042
Number of pages6
JournalLab on a Chip
Volume17
Issue number18
DOIs
Publication statusPublished - 2017 Sep 21
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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