Cell fusion assay by expression of respiratory syncytial virus (RSV) fusion protein to analyze the mutation of palivizumab-resistant strains

Yosuke Yasui, Yoshiaki Yamaji, Akihito Sawada, Takashi Ito, Tetsuo Nakayama

Research output: Contribution to journalArticle

Abstract

Respiratory syncytial virus (RSV) consists of fusion (F), glyco (G), and small hydrophobic (SH) proteins as envelope proteins, and infects through cell fusion. F protein is expressed on the surface of infected cells, and induces cell fusion. In the present report, expression plasmids of the F, G and SH proteins were constructed and cell fusion activity was investigated under T7 RNA polymerase. F protein alone induced cell fusion at a lower concentration than previously reported, and co-expression of F and SH proteins induced cell fusion more efficiently than F protein alone. Palivizumab is the only prophylactic agent against RSV infection. Palivizumab-resistant strains having mutations of the F protein of K272E and S275F were reported. These mutations were introduced into an F-expression plasmid, and exhibited no inhibition of cell fusion with palivizumab. Among the RSV F protein mutants, N276S has been reported to have partial resistance against palivizumab, but the F expression plasmid with the N276S mutation showed a reduction in cell fusion in the presence of palivizumab, showing no resistance to palivizumab. The present expression system was useful for investigating the mechanisms of RSV cell fusion.

Original languageEnglish
Pages (from-to)48-55
Number of pages8
JournalJournal of Virological Methods
Volume231
DOIs
Publication statusPublished - 2016 May 1

Fingerprint

Viral Fusion Proteins
Respiratory Syncytial Viruses
Cell Fusion
Mutation
F Factor
Proteins
Monomeric GTP-Binding Proteins
Respiratory Syncytial Virus Infections
Palivizumab
Mutant Proteins

Keywords

  • Cell fusion
  • F protein
  • G protein
  • Palivizumab resistant strain
  • Respiratory syncytial virus
  • SH protein

ASJC Scopus subject areas

  • Virology

Cite this

Cell fusion assay by expression of respiratory syncytial virus (RSV) fusion protein to analyze the mutation of palivizumab-resistant strains. / Yasui, Yosuke; Yamaji, Yoshiaki; Sawada, Akihito; Ito, Takashi; Nakayama, Tetsuo.

In: Journal of Virological Methods, Vol. 231, 01.05.2016, p. 48-55.

Research output: Contribution to journalArticle

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AU - Nakayama, Tetsuo

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