Abstract
Respiratory syncytial virus (RSV) consists of fusion (F), glyco (G), and small hydrophobic (SH) proteins as envelope proteins, and infects through cell fusion. F protein is expressed on the surface of infected cells, and induces cell fusion. In the present report, expression plasmids of the F, G and SH proteins were constructed and cell fusion activity was investigated under T7 RNA polymerase. F protein alone induced cell fusion at a lower concentration than previously reported, and co-expression of F and SH proteins induced cell fusion more efficiently than F protein alone. Palivizumab is the only prophylactic agent against RSV infection. Palivizumab-resistant strains having mutations of the F protein of K272E and S275F were reported. These mutations were introduced into an F-expression plasmid, and exhibited no inhibition of cell fusion with palivizumab. Among the RSV F protein mutants, N276S has been reported to have partial resistance against palivizumab, but the F expression plasmid with the N276S mutation showed a reduction in cell fusion in the presence of palivizumab, showing no resistance to palivizumab. The present expression system was useful for investigating the mechanisms of RSV cell fusion.
| Original language | English |
|---|---|
| Pages (from-to) | 48-55 |
| Number of pages | 8 |
| Journal | Journal of Virological Methods |
| Volume | 231 |
| DOIs | |
| Publication status | Published - 2016 May 1 |
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Keywords
- Cell fusion
- F protein
- G protein
- Palivizumab resistant strain
- Respiratory syncytial virus
- SH protein
ASJC Scopus subject areas
- Virology
Cite this
Cell fusion assay by expression of respiratory syncytial virus (RSV) fusion protein to analyze the mutation of palivizumab-resistant strains. / Yasui, Yosuke; Yamaji, Yoshiaki; Sawada, Akihito; Ito, Takashi; Nakayama, Tetsuo.
In: Journal of Virological Methods, Vol. 231, 01.05.2016, p. 48-55.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell fusion assay by expression of respiratory syncytial virus (RSV) fusion protein to analyze the mutation of palivizumab-resistant strains
AU - Yasui, Yosuke
AU - Yamaji, Yoshiaki
AU - Sawada, Akihito
AU - Ito, Takashi
AU - Nakayama, Tetsuo
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Respiratory syncytial virus (RSV) consists of fusion (F), glyco (G), and small hydrophobic (SH) proteins as envelope proteins, and infects through cell fusion. F protein is expressed on the surface of infected cells, and induces cell fusion. In the present report, expression plasmids of the F, G and SH proteins were constructed and cell fusion activity was investigated under T7 RNA polymerase. F protein alone induced cell fusion at a lower concentration than previously reported, and co-expression of F and SH proteins induced cell fusion more efficiently than F protein alone. Palivizumab is the only prophylactic agent against RSV infection. Palivizumab-resistant strains having mutations of the F protein of K272E and S275F were reported. These mutations were introduced into an F-expression plasmid, and exhibited no inhibition of cell fusion with palivizumab. Among the RSV F protein mutants, N276S has been reported to have partial resistance against palivizumab, but the F expression plasmid with the N276S mutation showed a reduction in cell fusion in the presence of palivizumab, showing no resistance to palivizumab. The present expression system was useful for investigating the mechanisms of RSV cell fusion.
AB - Respiratory syncytial virus (RSV) consists of fusion (F), glyco (G), and small hydrophobic (SH) proteins as envelope proteins, and infects through cell fusion. F protein is expressed on the surface of infected cells, and induces cell fusion. In the present report, expression plasmids of the F, G and SH proteins were constructed and cell fusion activity was investigated under T7 RNA polymerase. F protein alone induced cell fusion at a lower concentration than previously reported, and co-expression of F and SH proteins induced cell fusion more efficiently than F protein alone. Palivizumab is the only prophylactic agent against RSV infection. Palivizumab-resistant strains having mutations of the F protein of K272E and S275F were reported. These mutations were introduced into an F-expression plasmid, and exhibited no inhibition of cell fusion with palivizumab. Among the RSV F protein mutants, N276S has been reported to have partial resistance against palivizumab, but the F expression plasmid with the N276S mutation showed a reduction in cell fusion in the presence of palivizumab, showing no resistance to palivizumab. The present expression system was useful for investigating the mechanisms of RSV cell fusion.
KW - Cell fusion
KW - F protein
KW - G protein
KW - Palivizumab resistant strain
KW - Respiratory syncytial virus
KW - SH protein
UR - http://www.scopus.com/inward/record.url?scp=84960340639&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84960340639&partnerID=8YFLogxK
U2 - 10.1016/j.jviromet.2016.01.003
DO - 10.1016/j.jviromet.2016.01.003
M3 - Article
VL - 231
SP - 48
EP - 55
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
ER -