Cell migration and activated PI3K/AKT-directed elongation in the developing rat Müllerian duct

Akihiro Fujino, Nelson A. Arango, Yong Zhan, Thomas F. Manganaro, Xianlin Li, David T. MacLaughlin, Patricia K. Donahoe

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In vertebrates, the Müllerian duct elongates along the Wolffian duct, a mesonephric structure that is required for Müllerian duct formation. Recently, several genes required for initial Müllerian duct formation have been identified. However, the precise mechanism of Müllerian duct elongation remains to be elucidated. In this study, we investigated dynamic morphological changes in the elongating Müllerian duct in rat urogenital ridges in organ culture manipulated by microincision and/or chemical inhibitors. Mechanical division of the developing Müllerian duct showed that epithelial cells of the Müllerian duct actively migrate along the anterior-posterior axis independent of the proliferative expansion of the anterior portion of the duct. We found that the PI3K/AKT signaling pathway is activated in the Müllerian duct epithelium and is required for elongation of the tip of the duct; however, migration of Müllerian duct epithelial cells proximal to the tip remains intact when PI3K/AKT is inactivated. Although much is known about the molecular and cellular mechanisms leading to Müllerian duct regression, the present findings provide a fuller understanding of the mechanisms contributing to Müllerian duct formation and to the general process of early tubulogenesis.

Original languageEnglish
Pages (from-to)351-362
Number of pages12
JournalDevelopmental Biology
Volume325
Issue number2
DOIs
Publication statusPublished - 2009 Jan 15
Externally publishedYes

Fingerprint

Phosphatidylinositol 3-Kinases
Cell Movement
Epithelial Cells
Wolffian Ducts
Organ Culture Techniques
Vertebrates
Epithelium
Genes

Keywords

  • Cell migration
  • Coelomic epithelium
  • Duct elongation
  • LY294002
  • Müllerian duct
  • Phosphorylated AKT
  • PI3K/AKT pathway
  • Tubulerization
  • Wolffian duct

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Fujino, A., Arango, N. A., Zhan, Y., Manganaro, T. F., Li, X., MacLaughlin, D. T., & Donahoe, P. K. (2009). Cell migration and activated PI3K/AKT-directed elongation in the developing rat Müllerian duct. Developmental Biology, 325(2), 351-362. https://doi.org/10.1016/j.ydbio.2008.10.027

Cell migration and activated PI3K/AKT-directed elongation in the developing rat Müllerian duct. / Fujino, Akihiro; Arango, Nelson A.; Zhan, Yong; Manganaro, Thomas F.; Li, Xianlin; MacLaughlin, David T.; Donahoe, Patricia K.

In: Developmental Biology, Vol. 325, No. 2, 15.01.2009, p. 351-362.

Research output: Contribution to journalArticle

Fujino, A, Arango, NA, Zhan, Y, Manganaro, TF, Li, X, MacLaughlin, DT & Donahoe, PK 2009, 'Cell migration and activated PI3K/AKT-directed elongation in the developing rat Müllerian duct', Developmental Biology, vol. 325, no. 2, pp. 351-362. https://doi.org/10.1016/j.ydbio.2008.10.027
Fujino, Akihiro ; Arango, Nelson A. ; Zhan, Yong ; Manganaro, Thomas F. ; Li, Xianlin ; MacLaughlin, David T. ; Donahoe, Patricia K. / Cell migration and activated PI3K/AKT-directed elongation in the developing rat Müllerian duct. In: Developmental Biology. 2009 ; Vol. 325, No. 2. pp. 351-362.
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