Cell Origami: Self-Folding of Three-Dimensional Cell-Laden Microstructures Driven by Cell Traction Force

Kaori Kuribayashi-Shigetomi, Hiroaki Onoe, Shoji Takeuchi

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

This paper describes a method of generating three-dimensional (3D) cell-laden microstructures by applying the principle of origami folding technique and cell traction force (CTF). We harness the CTF as a biological driving force to fold the microstructures. Cells stretch and adhere across multiple microplates. Upon detaching the microplates from a substrate, CTF causes the plates to lift and fold according to a prescribed pattern. This self-folding technique using cells is highly biocompatible and does not involve special material requirements for the microplates and hinges to induce folding. We successfully produced various 3D cell-laden microstructures by just changing the geometry of the patterned 2D plates. We also achieved mass-production of the 3D cell-laden microstructures without causing damage to the cells. We believe that our methods will be useful for biotechnology applications that require analysis of cells in 3D configurations and for self-assembly of cell-based micro-medical devices.

Original languageEnglish
Article numbere51085
JournalPLoS One
Volume7
Issue number12
DOIs
Publication statusPublished - 2012 Dec 12
Externally publishedYes

Fingerprint

traction (mechanics)
Traction
microstructure
Microstructure
cells
Hinges
Biotechnology
Self assembly
Geometry
Substrates
medical equipment
harness
methodology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Cell Origami : Self-Folding of Three-Dimensional Cell-Laden Microstructures Driven by Cell Traction Force. / Kuribayashi-Shigetomi, Kaori; Onoe, Hiroaki; Takeuchi, Shoji.

In: PLoS One, Vol. 7, No. 12, e51085, 12.12.2012.

Research output: Contribution to journalArticle

@article{acdc81c6a8e245eb848f1b00fb767742,
title = "Cell Origami: Self-Folding of Three-Dimensional Cell-Laden Microstructures Driven by Cell Traction Force",
abstract = "This paper describes a method of generating three-dimensional (3D) cell-laden microstructures by applying the principle of origami folding technique and cell traction force (CTF). We harness the CTF as a biological driving force to fold the microstructures. Cells stretch and adhere across multiple microplates. Upon detaching the microplates from a substrate, CTF causes the plates to lift and fold according to a prescribed pattern. This self-folding technique using cells is highly biocompatible and does not involve special material requirements for the microplates and hinges to induce folding. We successfully produced various 3D cell-laden microstructures by just changing the geometry of the patterned 2D plates. We also achieved mass-production of the 3D cell-laden microstructures without causing damage to the cells. We believe that our methods will be useful for biotechnology applications that require analysis of cells in 3D configurations and for self-assembly of cell-based micro-medical devices.",
author = "Kaori Kuribayashi-Shigetomi and Hiroaki Onoe and Shoji Takeuchi",
year = "2012",
month = "12",
day = "12",
doi = "10.1371/journal.pone.0051085",
language = "English",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

TY - JOUR

T1 - Cell Origami

T2 - Self-Folding of Three-Dimensional Cell-Laden Microstructures Driven by Cell Traction Force

AU - Kuribayashi-Shigetomi, Kaori

AU - Onoe, Hiroaki

AU - Takeuchi, Shoji

PY - 2012/12/12

Y1 - 2012/12/12

N2 - This paper describes a method of generating three-dimensional (3D) cell-laden microstructures by applying the principle of origami folding technique and cell traction force (CTF). We harness the CTF as a biological driving force to fold the microstructures. Cells stretch and adhere across multiple microplates. Upon detaching the microplates from a substrate, CTF causes the plates to lift and fold according to a prescribed pattern. This self-folding technique using cells is highly biocompatible and does not involve special material requirements for the microplates and hinges to induce folding. We successfully produced various 3D cell-laden microstructures by just changing the geometry of the patterned 2D plates. We also achieved mass-production of the 3D cell-laden microstructures without causing damage to the cells. We believe that our methods will be useful for biotechnology applications that require analysis of cells in 3D configurations and for self-assembly of cell-based micro-medical devices.

AB - This paper describes a method of generating three-dimensional (3D) cell-laden microstructures by applying the principle of origami folding technique and cell traction force (CTF). We harness the CTF as a biological driving force to fold the microstructures. Cells stretch and adhere across multiple microplates. Upon detaching the microplates from a substrate, CTF causes the plates to lift and fold according to a prescribed pattern. This self-folding technique using cells is highly biocompatible and does not involve special material requirements for the microplates and hinges to induce folding. We successfully produced various 3D cell-laden microstructures by just changing the geometry of the patterned 2D plates. We also achieved mass-production of the 3D cell-laden microstructures without causing damage to the cells. We believe that our methods will be useful for biotechnology applications that require analysis of cells in 3D configurations and for self-assembly of cell-based micro-medical devices.

UR - http://www.scopus.com/inward/record.url?scp=84871247881&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871247881&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0051085

DO - 10.1371/journal.pone.0051085

M3 - Article

C2 - 23251426

AN - SCOPUS:84871247881

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 12

M1 - e51085

ER -