TY - JOUR
T1 - Cell Patterning Method on a Clinically Ubiquitous Culture Dish Using Acoustic Pressure Generated from Resonance Vibration of a Disk-Shaped Ultrasonic Transducer
AU - Imashiro, Chikahiro
AU - Kurashina, Yuta
AU - Kuribara, Taiki
AU - Hirano, Makoto
AU - Totani, Kiichiro
AU - Takemura, Kenjiro
N1 - Funding Information:
Manuscript received March 13, 2018; accepted May 5, 2018. Date of publication May 14, 2018; date of current version December 19, 2018. This work was supported by the JSPS KAKENHI under Grant JP16H04259 and Grant JP17H07081; and in part by the MEXT Grant-in-Aid for the Program for Leading Graduate Schools. (Corresponding author: Kenjiro Takemura.) C. Imashiro is with the School of Science for Open and Environmental Systems, Graduate School of Science and Technology, Keio University.
Publisher Copyright:
© 1964-2012 IEEE.
PY - 2019/1
Y1 - 2019/1
N2 - Cell patterning methods have been previously reported for cell culture. However, these methods use inclusions or devices that are not used in general cell culture and that might affect cell functionality. Here, we report a cell patterning method that can be conducted on a general cell culture dish without any inclusions by employing a resonance vibration of a disk-shaped ultrasonic transducer located under the dish. A resonance vibration with a single nodal circle patterned C2C12 myoblasts into a circular shape on the dish with 10-min exposure of the vibration with maximum peak-peak amplitude of 10 μm${}-{\text{p-p}}$. Furthermore, the relationship between the amplitude distribution of the transducer and the cell density in the patterned sample could be expressed as a linear function, and there was a clear threshold of amplitude for cell adhesion. To evaluate the cell function of the patterned cells, we conducted proliferation and protein assays at 120-h culture after patterning. Our results showed that the cell proliferation rate did not decrease and the expression of cellular proteins was unchanged. Thus, we conclude, this method can successfully pattern cells in the clinically ubiquitous culture dish, while maintaining cell functionality.
AB - Cell patterning methods have been previously reported for cell culture. However, these methods use inclusions or devices that are not used in general cell culture and that might affect cell functionality. Here, we report a cell patterning method that can be conducted on a general cell culture dish without any inclusions by employing a resonance vibration of a disk-shaped ultrasonic transducer located under the dish. A resonance vibration with a single nodal circle patterned C2C12 myoblasts into a circular shape on the dish with 10-min exposure of the vibration with maximum peak-peak amplitude of 10 μm${}-{\text{p-p}}$. Furthermore, the relationship between the amplitude distribution of the transducer and the cell density in the patterned sample could be expressed as a linear function, and there was a clear threshold of amplitude for cell adhesion. To evaluate the cell function of the patterned cells, we conducted proliferation and protein assays at 120-h culture after patterning. Our results showed that the cell proliferation rate did not decrease and the expression of cellular proteins was unchanged. Thus, we conclude, this method can successfully pattern cells in the clinically ubiquitous culture dish, while maintaining cell functionality.
KW - Biotechnology
KW - cell patterning
KW - ultrasonic transducer
KW - underwater ultrasound
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U2 - 10.1109/TBME.2018.2835834
DO - 10.1109/TBME.2018.2835834
M3 - Article
C2 - 29993416
AN - SCOPUS:85046803746
VL - 66
SP - 111
EP - 118
JO - IRE transactions on medical electronics
JF - IRE transactions on medical electronics
SN - 0018-9294
IS - 1
M1 - 8358707
ER -