Cell Patterning Method on a Clinically Ubiquitous Culture Dish Using Acoustic Pressure Generated from Resonance Vibration of a Disk-Shaped Ultrasonic Transducer

Chikahiro Imashiro, Yuta Kurashina, Taiki Kuribara, Makoto Hirano, Kiichiro Totani, Kenjiro Takemura

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Cell patterning methods have been previously reported for cell culture. However, these methods use inclusions or devices that are not used in general cell culture and that might affect cell functionality. Here, we report a cell patterning method that can be conducted on a general cell culture dish without any inclusions by employing a resonance vibration of a disk-shaped ultrasonic transducer located under the dish. A resonance vibration with a single nodal circle patterned C2C12 myoblasts into a circular shape on the dish with 10-min exposure of the vibration with maximum peak-peak amplitude of 10 μm${}-{\text{p-p}}$. Furthermore, the relationship between the amplitude distribution of the transducer and the cell density in the patterned sample could be expressed as a linear function, and there was a clear threshold of amplitude for cell adhesion. To evaluate the cell function of the patterned cells, we conducted proliferation and protein assays at 120-h culture after patterning. Our results showed that the cell proliferation rate did not decrease and the expression of cellular proteins was unchanged. Thus, we conclude, this method can successfully pattern cells in the clinically ubiquitous culture dish, while maintaining cell functionality.

Original languageEnglish
Article number8358707
Pages (from-to)111-118
Number of pages8
JournalIEEE Transactions on Biomedical Engineering
Volume66
Issue number1
DOIs
Publication statusPublished - 2019 Jan

Keywords

  • Biotechnology
  • cell patterning
  • ultrasonic transducer
  • underwater ultrasound

ASJC Scopus subject areas

  • Biomedical Engineering

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