Cell response to micropatterned surfaces produced with polymeric microspheres

M. Miyaki, Keiji Fujimoto, H. Kawaguchi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Surface topography plays an important role in cell function. We fabricated microstructured patterns from monolayers of polymeric microspheres, which were arranged on a flat polystyrene dish. Such patterns could be quickly formed by spin-coating microspheres and were easily controlled by changing the size of the settled microspheres. Polystyrene (PS) microspheres having different sizes were prepared by soap-free emulsion polymerization in the presence of sodium chloride with different concentrations. The zeta potential of these microspheres was approx. -25 mV regardless of their diameter. Electron microscopic observations revealed that the microspheres were deposited on a PS dish in hexagonally-packed monolayers with a pitch equal to the diameter of each microsphere. The water contact angles gradually decreased with an increase of the pitch. To investigate the topographical effect of these surfaces on the neutrophil-like cell function, microscopic observations and measurements of active oxygen released from cells were carried out. Microscopic observation revealed that spreading cells adhered on PS microsphere-settling surfaces as well as on a PS film. As for active oxygen release, cell response to a PS microsphere-settling surface depended on the diameter of settling microspheres and showed a maximum for diameters of approx. 1 μm. This tendency was observed in phosphate-buffered solutions both with and without serum. Continuous activation which followed by the initial attachment seems to relate to the change of cytoskeletal systems. These results indicate that cells recognize the roughness of the micropatterned surface covered with PS microspheres, and a pitch between the tops of settled microspheres is an important factor for cell recognition. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)603-608
Number of pages6
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume153
Issue number1-3
DOIs
Publication statusPublished - 1999 Aug 15

Fingerprint

Microspheres
polystyrene
Polystyrenes
cells
settling
parabolic reflectors
neutrophils
soaps
oxygen
sodium chlorides
Monolayers
Reactive Oxygen Species
serums
emulsions
attachment
coating
phosphates
topography
tendencies
roughness

Keywords

  • Active oxygen
  • Micropatterned
  • Microsphere
  • Polystyrene
  • Surface topography

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

Cell response to micropatterned surfaces produced with polymeric microspheres. / Miyaki, M.; Fujimoto, Keiji; Kawaguchi, H.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 153, No. 1-3, 15.08.1999, p. 603-608.

Research output: Contribution to journalArticle

@article{89cc4702bca14d29b891440ea2088cc4,
title = "Cell response to micropatterned surfaces produced with polymeric microspheres",
abstract = "Surface topography plays an important role in cell function. We fabricated microstructured patterns from monolayers of polymeric microspheres, which were arranged on a flat polystyrene dish. Such patterns could be quickly formed by spin-coating microspheres and were easily controlled by changing the size of the settled microspheres. Polystyrene (PS) microspheres having different sizes were prepared by soap-free emulsion polymerization in the presence of sodium chloride with different concentrations. The zeta potential of these microspheres was approx. -25 mV regardless of their diameter. Electron microscopic observations revealed that the microspheres were deposited on a PS dish in hexagonally-packed monolayers with a pitch equal to the diameter of each microsphere. The water contact angles gradually decreased with an increase of the pitch. To investigate the topographical effect of these surfaces on the neutrophil-like cell function, microscopic observations and measurements of active oxygen released from cells were carried out. Microscopic observation revealed that spreading cells adhered on PS microsphere-settling surfaces as well as on a PS film. As for active oxygen release, cell response to a PS microsphere-settling surface depended on the diameter of settling microspheres and showed a maximum for diameters of approx. 1 μm. This tendency was observed in phosphate-buffered solutions both with and without serum. Continuous activation which followed by the initial attachment seems to relate to the change of cytoskeletal systems. These results indicate that cells recognize the roughness of the micropatterned surface covered with PS microspheres, and a pitch between the tops of settled microspheres is an important factor for cell recognition. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.",
keywords = "Active oxygen, Micropatterned, Microsphere, Polystyrene, Surface topography",
author = "M. Miyaki and Keiji Fujimoto and H. Kawaguchi",
year = "1999",
month = "8",
day = "15",
doi = "10.1016/S0927-7757(98)00623-2",
language = "English",
volume = "153",
pages = "603--608",
journal = "Colloids and Surfaces A: Physicochemical and Engineering Aspects",
issn = "0927-7757",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Cell response to micropatterned surfaces produced with polymeric microspheres

AU - Miyaki, M.

AU - Fujimoto, Keiji

AU - Kawaguchi, H.

PY - 1999/8/15

Y1 - 1999/8/15

N2 - Surface topography plays an important role in cell function. We fabricated microstructured patterns from monolayers of polymeric microspheres, which were arranged on a flat polystyrene dish. Such patterns could be quickly formed by spin-coating microspheres and were easily controlled by changing the size of the settled microspheres. Polystyrene (PS) microspheres having different sizes were prepared by soap-free emulsion polymerization in the presence of sodium chloride with different concentrations. The zeta potential of these microspheres was approx. -25 mV regardless of their diameter. Electron microscopic observations revealed that the microspheres were deposited on a PS dish in hexagonally-packed monolayers with a pitch equal to the diameter of each microsphere. The water contact angles gradually decreased with an increase of the pitch. To investigate the topographical effect of these surfaces on the neutrophil-like cell function, microscopic observations and measurements of active oxygen released from cells were carried out. Microscopic observation revealed that spreading cells adhered on PS microsphere-settling surfaces as well as on a PS film. As for active oxygen release, cell response to a PS microsphere-settling surface depended on the diameter of settling microspheres and showed a maximum for diameters of approx. 1 μm. This tendency was observed in phosphate-buffered solutions both with and without serum. Continuous activation which followed by the initial attachment seems to relate to the change of cytoskeletal systems. These results indicate that cells recognize the roughness of the micropatterned surface covered with PS microspheres, and a pitch between the tops of settled microspheres is an important factor for cell recognition. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.

AB - Surface topography plays an important role in cell function. We fabricated microstructured patterns from monolayers of polymeric microspheres, which were arranged on a flat polystyrene dish. Such patterns could be quickly formed by spin-coating microspheres and were easily controlled by changing the size of the settled microspheres. Polystyrene (PS) microspheres having different sizes were prepared by soap-free emulsion polymerization in the presence of sodium chloride with different concentrations. The zeta potential of these microspheres was approx. -25 mV regardless of their diameter. Electron microscopic observations revealed that the microspheres were deposited on a PS dish in hexagonally-packed monolayers with a pitch equal to the diameter of each microsphere. The water contact angles gradually decreased with an increase of the pitch. To investigate the topographical effect of these surfaces on the neutrophil-like cell function, microscopic observations and measurements of active oxygen released from cells were carried out. Microscopic observation revealed that spreading cells adhered on PS microsphere-settling surfaces as well as on a PS film. As for active oxygen release, cell response to a PS microsphere-settling surface depended on the diameter of settling microspheres and showed a maximum for diameters of approx. 1 μm. This tendency was observed in phosphate-buffered solutions both with and without serum. Continuous activation which followed by the initial attachment seems to relate to the change of cytoskeletal systems. These results indicate that cells recognize the roughness of the micropatterned surface covered with PS microspheres, and a pitch between the tops of settled microspheres is an important factor for cell recognition. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.

KW - Active oxygen

KW - Micropatterned

KW - Microsphere

KW - Polystyrene

KW - Surface topography

UR - http://www.scopus.com/inward/record.url?scp=0032786967&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032786967&partnerID=8YFLogxK

U2 - 10.1016/S0927-7757(98)00623-2

DO - 10.1016/S0927-7757(98)00623-2

M3 - Article

AN - SCOPUS:0032786967

VL - 153

SP - 603

EP - 608

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

SN - 0927-7757

IS - 1-3

ER -