Cell-sized confinement controls generation and stability of a protein wave for spatiotemporal regulation in cells

Shunshi Kohyama, Natsuhiko Yoshinaga, Miho Yanagisawa, Kei Fujiwara, Nobuhide Doi

Research output: Contribution to journalArticlepeer-review

Abstract

Min system, which determines the cell division plane of bacteria, uses the localization change of protein (Min wave) emerged by a reaction-diffusion coupling. Although previous studies have shown that cell-sized space and boundaries modulate shape and speed of Min waves, its effects on Min wave emergence was still elusive. Here, by using a fully confined microsized space as a mimic of live cells, we revealed that confinement changes conditions for Min wave emergence. In the microsized space, an increase of surface-to-volume ratio changed the localization efficiency of proteins on membranes, and therefore, suppression of the localization change was necessary to produce stable Min wave generations. Furthermore, we showed that the cell-sized space more strictly limits parameters for wave emergence because confinement inhibits instability and excitability of the system. These results illuminate that confinement of reaction-diffusion systems works as a controller of spatiotemporal patterns in live cells.

Original languageEnglish
JournalUnknown Journal
DOIs
Publication statusPublished - 2019 May 17

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Fingerprint Dive into the research topics of 'Cell-sized confinement controls generation and stability of a protein wave for spatiotemporal regulation in cells'. Together they form a unique fingerprint.

Cite this