Cell-Sized confinement in microspheres accelerates the reaction of gene expression

Ayako Kato, Miho Yanagisawa, Yuko T. Sato, Kei Fujiwara, Kenichi Yoshikawa

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Cell-sized water-in-oil droplet covered by a lipid layer was used to understand how lipid membranes affect biochemical systems in living cells. Here, we report a remarkable acceleration of gene expression in a cell-sized water-in-oil droplet entrapping a cell-free translation system to synthesize GFP (green fluorescent protein). The production rate of GFP (V GFP) in each droplet remained almost constant at least for on the order of a day, which implies 0th-order reaction kinetics. Interestingly, V GFP was inversely proportional to radius of droplets (R) when R is under 50μm, and V GFP in droplets with R ∼ 10μm was more than 10 times higher than that in the bulk. The acceleration rates of GFP production in cell-sized droplets strongly depended on the lipid types. These results demonstrate that the membrane surface has the significant effect to facilitate protein production, especially when the scale of confinement is on the order of cell-size.

Original languageEnglish
Article number283
JournalScientific Reports
Volume2
DOIs
Publication statusPublished - 2012
Externally publishedYes

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Green Fluorescent Proteins
Microspheres
Gene Expression
Oils
Lipids
Cell-Free System
Water
Membrane Lipids
Cell Size
Membranes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Cell-Sized confinement in microspheres accelerates the reaction of gene expression. / Kato, Ayako; Yanagisawa, Miho; Sato, Yuko T.; Fujiwara, Kei; Yoshikawa, Kenichi.

In: Scientific Reports, Vol. 2, 283, 2012.

Research output: Contribution to journalArticle

Kato, Ayako ; Yanagisawa, Miho ; Sato, Yuko T. ; Fujiwara, Kei ; Yoshikawa, Kenichi. / Cell-Sized confinement in microspheres accelerates the reaction of gene expression. In: Scientific Reports. 2012 ; Vol. 2.
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